| Project/Area Number |
13470498
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
医薬分子機能学
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| Research Institution | Nagoya City University |
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Principal Investigator |
KATO Koichi Nagoya City University, Department of Pharmaceutical Sciences, Professor, 大学院・薬学研究科, 教授 (20211849)
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| Co-Investigator(Kenkyū-buntansha) |
KURIMOTO Eiji Nagoya City University, Department of Pharmaceutical Sciences, Assistant, 大学院・薬学研究科, 助手 (90234575)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2002: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2001: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | glycoprotein / NMR / multi-dimensional HPLC mapping / calreticulin / rheumatoid factor / immunoglobulin / CD1 / Fc receptor |
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| Research Abstract |
Sugar chains of glycoconjugates mediate molecular recognition involved in cell-cell communication and contribute to folding and quality control of glycoproteins. Although biological significance of sugar chains were widely recognized, little was known about their specific roles from a structural aspect. In the present study, we have developed nuclear magnetic resonance (NMR) and multi-dimensional HPLC methods for the elucidation of biological functions of sugar chains of glycoconjugates of immunological interests. By use of these techniques, we have addressed the following issues :
1) Modes of interactions between sugar chains and intracellular lectins such as calreticulin and VIP-36.
2) Roles of the sugar chains attached to the Fc portion of immunoglobulin G (IgG) in the interactions with Fcγ receptors and rheumatoid factors.
3) NMR and HPLC analyses of the carbohydrate moieties expressed by recombinant CD1 glycoproteins.
4) Glycosylation profiling of serum IgGs under a variety of pathological conditions.
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