| Project/Area Number |
13470510
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | Tohoku University |
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Principal Investigator |
YAMAZOE Yasushi Tohoku Univ., Grad. Sch. of Pharm. Sci., Prof., 大学院・薬学研究科, 教授 (00112699)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Masayuki Otsuka Pharmaceutical Co., Ltd., Res., 研究員
MIYATA Masaaki Tohoku Univ., Grad. Sch. of Pharm. Sci., assist., 大学院・薬学研究科, 助手 (90239418)
NAGATA Kiyoshi Tohoku Univ., Grad. Sch. of Pharm. Sci., Ass. Prof., 大学院・薬学研究科, 助教授 (80189133)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2002: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2001: ¥9,000,000 (Direct Cost: ¥9,000,000)
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| Keywords | enzyme induction / stable expression / CYP3A4 / human drug metabolism / PXR / 誘導評価系 / 安定発現株 / レポーターアッセイ / スクリーニング / in vivo / CYP3A / cyt.P450 / Induction / PXR |
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| Research Abstract |
As an estimation tool for human CYP3A4 induction, we have succeeded to make an adenovirus vecter system including CYP3A4 promoter and enhancer regions. The vecter was used to obtain the stably active clones in HepG2 cells. Among the clones selected, couple clones responded to typical chemical induces and showed high luciferase reporter activities. These clones are found to be candidates as effective tools for the quantitative prediction of inducing potential for human CYP3A4. We are currently evaluating the clones for the practical tools.
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