| Project/Area Number |
13470514
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TAKAKURA Yoshinobu Kyoto University, Grad. Sch. Pharm. Sci., Professor, 薬学研究科, 教授 (30171432)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAOKA Kiyoshi Kyoto University, Grad. Sch. Pharm. Sci., Associate Professor, 薬学研究科, 助教授 (50109013)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 2002: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | DNA vaccine / plasmid DNA / macrophage / dendritic cell / cationic carrier / local administration / antibody production / カチオン性高分子 / 抗原提示細胞 / 細胞取り込み / サイトカイン / CpGモチーフ |
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| Research Abstract |
To construct a strategy for optimizing plasmid DNA delivery for DNA vaccination, a series of in vitro and in vivo studies were carried out. In vitro studies using cultured macrophages and dendritic cells demonstrated that a specific mechanism for polyanions would be involved in these cells. In vitro cellular activation experiments revealed that dendritic cells were activated by naked plasmid DNA and its cationic liposome complexes in CpG motif-dependent manner. On the other hand, macrophage activation by naked DNA was dependent on CpG motif while DNA can stimulate macrophages in a CpG motif-independent manner when it is complexed with the cationic liposomes. In vivo local immunization studies using mice demonstrated that plasmid DNA complexed with a cationic carrier, methylated bovine serum albumin can enhance antibody induction with DNA vaccination, suggesting that immune reactions can be modulated by the control of local disposition of plasmid DNA with complexation with the carrier, Thus, the present study provides useful in vitro and in vivo information about the DNA vaccination approach.
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