| Project/Area Number |
13480151
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | IBARAKI UNIVERSITY |
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Principal Investigator |
OHTA Hiroyuki IBARAKI Univ., College of Agriculture, Professor, 農学部, 教授 (80168947)
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| Co-Investigator(Kenkyū-buntansha) |
KURUSU Yasurou IBARAKI Univ., College of Agriculture, Professor, 農学部, 教授 (60272118)
SHIRAI Makoto IBARAKI Univ., College of Agriculture, Professor, 農学部, 教授 (10007792)
KUBOTA Masatsugu IBARAKI Univ., College of Agriculture, Professor, 農学部, 教授 (40007688)
HASEGAWA Morifumi IBARAKI Univ., College of Agriculture, Research Associate, 農学部, 助手 (80311588)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2001: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | nonylphenol / endocrine disruptors / POP-degrading bacteria / Sphingobium / Sphingomanas / alkylphenol hydroxylase / degradation metabolism / スフィンゴビウム・アミエンセ / オクチルフェノール / 分解菌の環境動態 / スフィンゴモナス属細菌 / 分解菌の動態 |
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| Research Abstract |
The results of this study are summarized as follows:
1.Isolation and characterization of a nonylphenol-degrading bacterium from environmental samples
A Gram-negative bacterium (strain YT) able to degrade branched nonylphenol was isolated from a river sediment sample and characterized chemotaxonomically and phylogenetically. From the results, we propose that strain YT should be classified as a novel species, Sphingocium amiensis sp. nov.
2.The metabolic pathway of nonylphenol degradation in S.amiense strain YT was analyzed. Two types of alkylphenol hydroxylases were identified in the strain
3.Degradation profiles of alkylphenols by two nonyphenol-degrading bacteria, S.amiense YT and Sphingomonas cloacae JCM 10874, were analyzed.
4.Genome sizes of two nonylphenol-degrading bacteria, S.Amiense YT and Sphingomonas cloacae JCM 10874, were determined.
5.Two plasmids with the size of about 200 kb and 10 kb were found in each of two nonylphenol-degrading bacteria, S.Amiense YT and Sphingomonas cloacae JCM 10874. The 10 kb plasmid from S.Amiense YT was analyzed and applied to develop for useful vector among the Sphingomonas group bacteria from environments.
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