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Biochemical and genetic analyses of nonylphenol degradation metabolism in environmental bacteria

Research Project

Project/Area Number 13480151
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental dynamic analysis
Research InstitutionIBARAKI UNIVERSITY

Principal Investigator

OHTA Hiroyuki  IBARAKI Univ., College of Agriculture, Professor, 農学部, 教授 (80168947)

Co-Investigator(Kenkyū-buntansha) KURUSU Yasurou  IBARAKI Univ., College of Agriculture, Professor, 農学部, 教授 (60272118)
SHIRAI Makoto  IBARAKI Univ., College of Agriculture, Professor, 農学部, 教授 (10007792)
KUBOTA Masatsugu  IBARAKI Univ., College of Agriculture, Professor, 農学部, 教授 (40007688)
HASEGAWA Morifumi  IBARAKI Univ., College of Agriculture, Research Associate, 農学部, 助手 (80311588)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2001: ¥6,600,000 (Direct Cost: ¥6,600,000)
Keywordsnonylphenol / endocrine disruptors / POP-degrading bacteria / Sphingobium / Sphingomanas / alkylphenol hydroxylase / degradation metabolism / スフィンゴビウム・アミエンセ / オクチルフェノール / 分解菌の環境動態 / スフィンゴモナス属細菌 / 分解菌の動態
Research Abstract

The results of this study are summarized as follows:
1.Isolation and characterization of a nonylphenol-degrading bacterium from environmental samples
A Gram-negative bacterium (strain YT) able to degrade branched nonylphenol was isolated from a river sediment sample and characterized chemotaxonomically and phylogenetically. From the results, we propose that strain YT should be classified as a novel species, Sphingocium amiensis sp. nov.
2.The metabolic pathway of nonylphenol degradation in S.amiense strain YT was analyzed. Two types of alkylphenol hydroxylases were identified in the strain
3.Degradation profiles of alkylphenols by two nonyphenol-degrading bacteria, S.amiense YT and Sphingomonas cloacae JCM 10874, were analyzed.
4.Genome sizes of two nonylphenol-degrading bacteria, S.Amiense YT and Sphingomonas cloacae JCM 10874, were determined.
5.Two plasmids with the size of about 200 kb and 10 kb were found in each of two nonylphenol-degrading bacteria, S.Amiense YT and Sphingomonas cloacae JCM 10874. The 10 kb plasmid from S.Amiense YT was analyzed and applied to develop for useful vector among the Sphingomonas group bacteria from environments.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] De Vries, Y.P., Takahara, Y., Ikunaga, Y., Ushiba, Y., Hasegawa, M., Kasahara, Y., Shimomura, H., Hayashi, S., Hirai, Y., Ohta, H.: "Organic nutrient-dependent degradation of branched nonylphenol by Sphingomonas sp.YT isolated from a river sediment sample"Microbes and Environments. 16巻・4号. 240-249 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 太田寛行, 高原義治, 生長陽子, 牛場裕司, 宮川修一, 長谷川守文, 笠原康裕, 久留主泰朗, 小野勝道, 久保田俊夫: "ノニルフェノール類化学物質による環境汚染と分解微生物:土壌における実態の解明に向けて"日本微生物生態学会誌. 17巻・2号. 29-37 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ushiba, Y., Takahara, Y., Ohta, H.: "Sphingobium amiense sp.nov., a novel nonylphenol-degrading bacterium isolated from a river sediment"International Journal of Systematic and Evolutionary Microbiology. 53巻・6号. 2045-2048 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikunaga, Y., Hasegawa, M., Kasahara, Y., Ohta, H.他共著: "Biogeochemical Aspects of Earth System and Bioremediation of Polluted Environments"The 16th International Symposium on Environmental Biogeochemistry and its OECD Session, Sendai, Japan. 124 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] de Vries, Ynte P., Takahara, Y., Ikunaga, Y., Ushiba, Y., Hasegawa, M., Kasahara, Y., Shimomura, H., Hayashi, S., Hirai, Y., Ohta, H.: "Organic nutrient-dependent degradation of branched nonylphenol by Sphingomonas sp. YT isolated from a river sediment sample"Microbes and Environments. Vol.16. 240-249 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohta, H., Takahaara, Y., Ikunaga, Y., Ushiba, Y., Miyakawa, S., Hasegawa, M., Kasahara, Y., Kurusu, Y., Ono, K., Kubota, T.: "Environmental pollution by nonylphenol and its relative compounds and their degrading microbes: current knowledge about the pollution in soil"Bulletin of Japanese Society of Microbial Ecology. Vol.17. 29-37 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ushiba, Y., Takahara, Y., Ohta, H.: "Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment"International Journal of Systematic and Evolutionary Microbiology. Vol.53. 2045-2048 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikunaga, Y., Hasegawa, M., Kasahara, Y., Ohta, H.: "Physiological and biochemical comparison of two nonylphenol-degrading Sphingomonas strains"Biogeochemical Aspects of Earth System and Bioremediation of Polluted Environments (edited by Tsutomu Hattori). ISEB 16. 96 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ushiba, Y.Takahara, H.Ohta.: "Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment"International Journal of Systematic and Evolutionary Microbiology. 53巻・6号. 2045-2048 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 斎藤美有紀, 生長陽子, 太田寛行, 久留主泰朗: "Sphingomonas属細菌を宿主とするベクター系の開発"第19回日本微生物生態学会講演要旨集. 46 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Ikunaga, M.Hasegawa, Y.Kasahara, H.Ohta 他共著: "Biogeochemical Aspects of Earth System and Bioremediation of Polluted Environments"The 16th Internationa Symposium on Environmental Biogeochemistry and its OECD Session, ed. by Tsutomu Hattori Sendai, Japan. 124 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 太田寛行, 高原義治, 生長陽子, 牛場裕司, 宮川修一, 長谷川守文, 笠原康裕, 久留主泰朗, 小野勝道, 久保田俊夫: "ノニルフェノール類化合物質による環境汚染と分解微生物:土壌における実態の解明に向けて"日本微生物生態学会誌. 17巻・2号. 29-37 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 韓 宇波, 高原義治, 生長陽子, 太田寛行: "細菌混合培養系におけるノニルフェノール分解特性の解析"第18回日本微生物生態学会講演要旨集. 163 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 韓 宇波, 牛場裕司, 高原義治, 笠原康裕, 太田寛行: "ノイルフェノール(NP)分解性Sphingomonas sp. YTと共存するNP非分解性細菌の分類学的研究"第18回日本微生物生態学会講演要旨集. 177 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 生長陽子, 高原義治, 久留主泰朗, 長谷川守文, 白井 誠, 太田寛行: "高濃度及び低濃度ノニフェノール分解性Sphingomonas属細菌における分解特性の解析"第18回日本微生物生態学会講演要旨集. 80 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 宮川修一, 生長陽子, 長谷川守文, 太田寛行, 児玉 治: "Sphingomonas amiensisにおけるアルキルフェノール分解に関する研究"日本農薬学会第28回大会講演要旨集. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] De Vries YP, Takahara Y., Ikunaga Y., Ushiba Y., Hasegawa M., Kasahara Y., Shimomura H., Hayashi S., Hirai Y., Ohta H.: "Organic nutrient-dependent degradation of branched nonylphenol by Sphingomonas sp. YT isolated from a river sediment sample"Microbes and Environments. 16巻・4号. 240-249 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 牛場裕司, 高原義治, 太田寛行: "河川底質から分離されたノニルフェノール分解性Sphingomonas sp. YTの分類学的性質"第17回日本微生物生態学会講演要旨集. 136 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 生長陽子, 久留主泰朗, 笠原康裕, 太田寛行: "Sphingomonas sp. YT株の低濃度ノニルフェノールの分解性"第17回日本微生物生態学会講演要旨集. 78 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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