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Effects of diesel exhaust particles on cardiorespiratory health

Research Project

Project/Area Number 13480173
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

TAKANO Hirohisa  National Institute for Environmental Studies, Team Leader, 環境ホルモン・ダイオキシン研究プロジェクト, 総合研究官 (60281698)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Toshikaju  Kyoto Prefectural University of Medicine, Professor, 医学部, 教授 (90158410)
ICHINOSE Takamichi  Oita University for Nursing and Health Sciense, Professor, 看護学部, 教授 (50124334)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2002: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2001: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordsdiesel exhaust particles / particulate matter / nitrogen dioxide / lung injury / Circulatory disturbance / infection / bronchial asthoma / (aortic sclerosis) atherosclerosis / 生活習慣病 / 呼吸器障害 / 循環器障害 / 喘息 / サイトカイン / ディーゼル排気 / 呼吸器傷害 / 肺血管透過性 / 細菌毒素 / ケモカイン / NFкB
Research Abstract

Epidemiology studies demonstrate acute and serious adverse effects of particulate air pollution on cardiorespiratory health especially in the predisposed people susceptible to bacterial infection. We determined the effects of diesel exhaust particles (DEP), major participants in particulate pollutants, on lung injury related to bacterial endotoxin in mice. Intratracheal instillation of DEP synergistically enhanced lung injury related to endotoxin from gram-negative bacteria, which was characterized by neutrophil sequestration, interstitial edema, and alveolar hemorrhage. In the presence of endotoxin, DEP further activated the nuclear translocation of p65 subunit of NF-κ B in the lung, and increased the lung expression of ICAM-1, IL-1 β , macrophage chemoattractant protein-1, KC, macrophage inflammatory protein-1 α , and Toll-like receptors. DEP given alone increased the lung expression of Toll-like receptor 4 and the nuclear localization of p50 subunit of NF-κ B. These results provide the first experimental evidence that DEP enhance neutrophilic lung inflammation related to bacterial endotoxm. The enhancement should be mediated by the induction of proinflammatory molecules likely through the expression of Toll-like receptors and the activation of p65-containing dimer(s) of NF-κ B such as p65/p50 (reference 5).
Furthermore, we have shown DEP or DE enhance airway inflammation related to house dust mite allergen possibly via the enhanced expression of IL-5 and EOTAXIN (reference 6, 9). In another series of experiments, we have demonstrated that DE enhances both immediate and late airway responses as well as mucus secretion (reference 1). In addition, DEP can affect immune systems via the modification of oral tolerance or Th1/Th2 balance (reference 2, 3). Finally, we have demonstrated that nitrogen dioxide in DE can enhance atherogenic metabolic complications especially in obese subjects (submitted).

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Hashimoto K, Takano H, et al.: "Exposure to diesel exhaust excerbates allergen-induced airway responses in guine a pigs"American Journal of Respiratory and Critical Care Medicine. 164. 1957-1963 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshino S, Takano H, et al.: "Effect of diesel exhaust particle extracts on induction of oral tolerance in mice"Toxicological Sciences. 66. 293-297 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano H, et al.: "Diesel Exhaust Particles Enhance Lung Injury Related to Bacterial Endotoxin through Expression of Proinflammatory Cytokines, Chemokines, and ICAM-1"Americal Journal of Respiratory and Critical Care Medicine. 165. 1329-1335 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano H, et al.: "Lung expression of cytochrome P450 as a possible biomarker of exposure to diesel exhaust particles"Archines of Toxicology. 76. 146-151 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sadakane K, Takano H, et al.: "Marine strain differences in airway inflammation indned by diesel exhaust particles and house dust mite allergen"International Archines of Allergy and Immunology. 128. 220-228 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ichinose T, Takano H, et al.: "Differences in airway-inflammation development by house dust mice and diesel exhaust inhalation among mouse strains"Toxicol and Applied Pharmacology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 高野 裕久 他: "「ビタミンEと大気汚染」 ビタミンEの臨床"医薬ジャーナル社(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hashimoto K, Ishii Y, Uchida Y, Kimura T, Masuyama K, Morishima Y, Hirano K, Nomura A, Sakamoto T, Takano H, Sagai M, Sekizawa K: "Exposure to diesel exhaust exacerbates allergen-induced asthmatic responses in guinea pigs"Am J Respir Crit Care Med. 164. 1957-1963 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshino S, Hayashi H, Taneda S, Takano H, Sagai M, Mori Y: "Effects of diesel exhaust particle extracts on TH1 and TH2 immune response in mice"Int J Immunopathol Pharmacol. 15. 13-18 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshino S, Hayashi H, Taneda S, Takano H, Sagai M, Mori Y: "Effect of diesel exhaust particle extracts on induction of oral tolerance in mice"Toxicol Sci. 66. 293-297 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano H, Yanagisawa K, ichnose T, Sadakane K, Inoue K, Yoshida S, Takeda K, Yoshino S, Yoshikawa T, Morita M: "Lung expression of cytchrome P450 1A1 as a possible biomarker of exposure to diesel exhaust particles"Arch Toxicol. 76. 146-151 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano H, Yanagisawa R, Ichinose T, Sadakane K,Yeshino S, Yoshikawa T, Morita M: "Diesel exhaust particles enhance lung injury related to bacterial endotoxin through expression of proinflammatory cytokines, chemokines, and ICAM-1"Am J Respir Crit Care Med. 165. 1329-1335 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sadakane K, Ichinose T, Takano H, Yanagisawa R, Sagai M, Yoshikawa T, Shibamoto T: "Murine strain differences in airway inflammation induced by Diesel exhaust particles and house dust mite allergen"Int Arch Allergy Immunol. 128. 220-228 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ichinose T, Takano H, Miyabara Y, Sagai M, Sadakane K, Shibamoto T: "Diesels exhaust particles enhance antigen-induced eosinophilic inflammation in the airway of mast cell deficient mice"Toxicology. 180. 293-301 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sadakane K, Takano H, Ichinose T, Yanagisawa R, Shibamoto T: "Formaldehyde enhances mite allergen-induced eosinophilic inflammation in the murine airway"J Environmental Pathology Toxicology and Oncology. 21. 267-276 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ichinose T, Takano H, Sadakane K, Yanagisawa R, Kawazato H, Sagai M, Shibamoto T: "Differences in airway-inflammation development by house dust mite and diesel exhaust inhalation among mouse strains"Toxicol Applied Pharmacol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sanbongi C, Takano H, Osakabe N, Sasa N, Natsume M, Yanagisawa R, Inoue K, Yoshikawa T: "Rosmarinic acid inhabits lung injury induced by diesel exhaust particles"Free Radic Biol Med. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue K, Takano H, Yoshikawa T: "Inflammatory chemokine in the pathogenesis of the common cold"Lancet. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshino, S., Takano, H., et al.: "Effect of diesel exhaust particle extracts on induction of oral tolerance in mice"Toxicological Sciences. 60. 293-297 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano, H., et al.: "Diesel exhaust paticle enhance lung injury related to bacterial endotoxin through expression"American Journai of Respiratory and Criticai Care Medicine. 165. 1329-1335 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano, H., et al.: "Lung expression of cytochrome P450 as a possible biomarker of exposure to diesel exhaust particles"Archives of Toxicology. 76. 146-151 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sadakane, K., Takano, H., et al.: "Murine Strain diflerences in airway inflammation induced by diesel exhaust particles and house dust mite allergen"International Archives of Allergy and Immunology. 128. 220-228 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano H, et al.: "Diesel Exhaust Particles Enhance Lung Injury Related to Bacterial Endotoxin through Expression of Proinflammatory Cytokines, Chemokines, and Intercellular Adhesion Molecule-1"American Journal of Respiratory and Critical Care Medicine. (in press).

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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