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Analysis of peptide molecules that regulate Hydra morphogenesis

Research Project

Project/Area Number 13480249
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNational Institute of Genetics

Principal Investigator

FUJISAWA Toshitaka  National Institute of Genetics, Developmental Genetics, Associate professor, 個体遺伝研究系, 助教授 (60000262)

Co-Investigator(Kenkyū-buntansha) HATTA Masayuki  Ochanomizu University, Faculty of Science, Associate Professor, 理学部, 助教授 (00249947)
SHIMIZU Hiroshi  National Institute of Genetics, Developmental Genetics, Assistant Professor, 個体遺伝研究系, 助手 (60178986)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2001: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsHydra / Epitheliopeptides / Neuropeptides / Hydra ESTs / Gene expression analysis / GPCRs / 遺伝子発煙解析 / ペプチド性シグナル分子 / 組織的同定 / 受容体の同定 / 形態形成 / オーファンGPCR
Research Abstract

1.Systematic purification and identification of peptide signaling molecules in Hydra
We purified about 80 peptides and screened for biological activities including morphogenesis, cell proliferation, cell differentiation, behavior etc. We also cloned genes that encode peptides and analyzed their expression in whole mount in situ hybridization. At the same time, we identified many novel peptide genes from ESTs and examined their expression. Through these analyses, we identified 10 new neuropeptides that regulate various aspects of hydra behavior.
2.We newly initiated the Hydra EST Project and obtained 7,000 independent clones, which were arrayed into microarrays for further analysis.
3.Cloning of possible peptide receptors.
Receptors that use peptides as ligands are generally regarded as G-protein coulpled receptors with 7 tranmembrane domains (GPCRs). We have established mammalian cultured cells that express an individual Hydra GPCR. Using these cells we analyzed the changes of intracellular calcium concentration by microscopic means when hydra peptide libraries were added. At present, we were unable to detect any significant changes although positive control using the mouse melanin concentration hormone and its receptor system worked efficiently. In future, we will fractionalte hydra peptides that evoke calcium changes in GPCR expressing cells
4.Since this was the last year of this project, we put all the data together and are in the process of submitting a few papers.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Shimizu, H., Fujisawa, T.: "Peduncle of Hydra and the heart of higher organisms have a common ancestral origin."Genesis. 36. 182-186 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, T. et al.: "Identification of a new member of the LWamide peptide family : Physiologica actiivity and cellular localization in cnidarian polyps."Comparative Biochemistry and Physiology. 135. 309-324 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujisawa, T.: "Hydra regeneration and epitheliopeptides."Developmental Dynamics. 226. 182-189 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "Identification of a vasopressin-like immunoreactive substance in hydra"Peptides. 6452. 1-10 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimizu, H., Fujisawa, T.: "Peduncle of Hydra and the heart of higher organisms have a common ancestral origin."Genesis. 36. 182-186 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, T., Kobayakawa, Y., Muneoka, Y., Fujisawa, Y., Mohri, S., Shimizu, H., Fujisawa, T., Sugiyama, T., Takahara, M., Koizumi, O.: "Identification of a new member of the LWamide peptide family : Physiologica acitivity and cellular localization in cnidarian polyps."Comparative Biochemistry and Physiology. 135. 309-324 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujisawa, T.: "Hydra regeneration and epitheliopeptides"Developmental Dynamics. 226. 182-189 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morishita, F., Nitagai, Y., Furukawa, Y., Matsushima, O., Takahashi, T., Hatta, M., Fujisawa, T., Tsunamoto, S., Koizumi, O.: "Identification of a vasopressin-like immunoreactive substance in hydra."Peptides. 6452. 1-10 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimizu, H.: "Peduncle of Hydra and the heart of higher organisms have a common ancestral origin."Genesis. 36. 182-186 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi, T.: "Identification of a new member of the LWamide peptide family : Physiologica acitivity and cellular localization in cnidarian polyps."Comparative Biochemistry and Physiology. 135. 309-324 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujisawa, T.: "Hydra regeneration and epitheliopeptides."Developmental Dynamics. 226. 182-189 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Morishita, F.: "Identification of a vasopressin-like immunoreactive substance in hydra."Peptides. 6452. 1-10 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iwao, K: "A cnidarian neuropeptide of the GLWamide family induces metamorphosis of reef-building corals in the genus Acropora"Coral Reefs. 21. 127-129 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mochizuki, K.: "Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in Tetrahymena"Cell. 110. 689-699 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fabila, Y.: "Selective inhibition of protein kinases blocks the formation of new axis, the beginning of budding, in Hydra"Mechanism of Development. 119. 157-164 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujisawa, T.: "Hydra regeneration and epitheliopeptides"Developmental Dynamics. 226. 182-189 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morishita, F.: "Identification of a vasopressin-like immunoreactive substance in hydra"Peptides. 6452. 1-10 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi, T.: "Identification of a new member of the LWamide peptide family : Physiological activity and cellular localization in cnidarian polyps"Comparative Biochemistry and Physiology. (In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwao, K.: "A cnidarian neuropeptide of the GLWamide family induces metamorphosis of reef-building corals in the genus Acropora"Coral Reef. (in Press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Mochizuki, K.: "Universal occurrence of the vasa-related genes among netazoans and their germline expression in Hydra"Development, Genes and Evolution. 211. 299-308 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Bosch, T.C.G.: "Polyps, peptides and patterning"Bioessays. 23. 420-427 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Harafuji, N.: "Enhancement of foot formation in Hydra by a novel epitheliopeptide, Hym-323"Development. 128. 437-446 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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