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Molecular mechanism of strengthening synaptic connection by neural activity.

Research Project

Project/Area Number 13480261
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionTokyo Metropolitan Institute for Neuroscience

Principal Investigator

YAMAGATA Kanato  Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for Neuroscience, Staff Scientist, 東京都神経科学総合研究所, 副参事研究員 (20263262)

Co-Investigator(Kenkyū-buntansha) TANAKA Hidekazu  Osaka University Graduate School of Medicine, Department of Pharmacology, Assistant Professor, 大学院・医学系研究科, 助手 (70273638)
SUGIURA Hiroko  Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for Neuroscience, Staff Scientist, 東京都神経科学総合研究所, 研究員 (40162870)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2002: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2001: ¥9,300,000 (Direct Cost: ¥9,300,000)
Keywordsneural activity / cell adhesion molecule / cadherin / 神経接着分子 / MAP kinase
Research Abstract

We investigated the role of activity-regulated adhesion molecule arcadlin. Arcadlin is synthesized in neurons and carried to synapses and may be involved in synaptic reorganization stimulated by synaptic activity. Here, we analyzed the interaction of TAO2 with the cytoplasmic region of Arcadlin. Homophilic binding of extracellular (EC) domains of arcadlin activated TAO2 and its down-stream kinases, MKK3 and p38 MAP kinase. The homophilic interaction also induced the internalization of arcadlin itself. This internalization was completely blocked by the co-expression of dominant-negative dynamin, indicating that arcadlin was internalized by clathrin-mediated endocytosis. The absence of MKK3 and p38 MARK resulted in a loss of arcadlin endocytosis. Moreover, p38 MARK inhibitor SB203580 also inhibited the endocytosis of arcadlin. These results suggest that TAO2, stimulated by homophilic binding of arcadlin EC domains, activates p38 MAP kinase pathway and induces the endocytosis of arcadlin. Thus, neural activity may play a role in changing the strength of synaptic connections by regulating the number of cell adhesion molecules on the surface of synaptic membranes.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Matsuoka M. et al.: "Expression and regulation of the immediate-early gene product Arc in the accessory olfactory bulb after mating in male rat"Neuroscience. 111(2). 251-258 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsumura K. et al.: "COX-2 and mPGES in bain endothelial cells : potential targets of anti-inflammentory drugs"Current Medical Chemistry. 1. 161-166 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsuoka M. et al.: "Mating behavior induces different Arc expression in the main and accessory olfactory bulbs of adult rats"Neurosci Lett.. 335(2). 111-114 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue W. et al.: "Brain-specific endothelial induction of postaglandin E(2) synthesis enzymes and its temporal relation to fever"Neurosci Res. 44(1). 51-61 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamagata K. et al.: "Coexpression of microsomal-type prostaglandin E synthase with cyclooxygenase-2 in brain endothelial cells of rats during endotoxin-induced fever"J Neurosci.. 21(8). 2269-2277 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Uwai K.et al.: "Virol A, a toxic trans-polyacetylenic alcohol of Cicuta virosa, selectively inhibits the GABA-induced Cl(-) current in acutely dissociated rat hippocampal CAI neurons"Brain.Res.. 889(1-2). 174-180 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山形要人, 竹宮孝子: "アラキドン酸カスケードQ&A"医薬ジャーナル社. 178 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matusmura K, et al.: "COX-2 and mPGES in bain endothelial cells : potential targets of anti-inflammatory drugs"Current Medical Chemistry. 1. 161-166 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsuoka M, et al.: "Mating behavior induces differential Arc expression in the main and accessory olfactory bulbs of adult rats"Neurosci Lett.. 335(2). 111-114 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue W, et al.: "Brain-specific endothelial induction of prostaglandin E(2) synthesis cnzymes and its temporal relation to fever"Neurosci Res.. 44(1). 51-61 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsuoka M, et al.: "Expression and regulation of the immediate-early gene product Arc in the accessory olfactory bulb after mating in male rat"Neuroscience. 111(2). 251-258 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamagata K, et al.: "Coexpression of microsomal-type prostaglandin E synthase with cyclooxygenase-2 in brain endotherial cells of rats during endotoxm-induced fever"J. Neurosci.. 21(8). 2669-2677 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Uwai K, et al.: "Virol A, a toxic trans-polyacetylenic alcohol of Cicuta virosa, selectively inhibits the GABA-induced Cl(-) current in acutely dissociated rat hippocampal Cal neurons"Brain Res.. 889(1-2). 174-180 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsuoka M. et al.: "Expression and regulation of the immediate-early gene product Arc in the accessory olfactory bulb after mating in male rat"Neuroscience. 111(2). 251-258 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsumura K et al.: "COX-2 and mPGES in bain endothelial cells : potential targets of anti-inflammatory drugs"Current Medical Chemistry. 1. 161-166 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsuoka M. et al.: "Mating behavior induces differential Arc expression in the main and accessory olfactory bulbs of adult rats"Neurosci Lett.. 335(2). 111-114 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inoue W. et al.: "Brain-specific endothelial induction of prostaglandin E(2) synthesis enzymes and its temporal relation to fever"Neurosci Res.. 44(1). 51-61 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山形要人, 竹宮孝子: "アラキドン酸カスケードQ&A"医薬ジャーナル社. 178 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamagata K et al.: "Coexpression of microsomal-type prostaglandin E synthase with cyclooxygenase-2 in brain endothelial cells of rats during endotoxin-induced fever."J Neuro sci.. 21(8). 2669-2677 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uwai K et al.: "Virol A. a toxic trans-polyacetylenic alcohol of Cicuta virosa, selectively inhibits the GABA-induced CI(-) current in acutely dissociated rat hippocampal CAl neurons."Brain Res.. 889(1-2). 174-180 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Phillips GR et al.: "The presynaptic particle web : ultrastructure, composition, dissolution, and reconstitution."Neuron. 32(1). 63-77 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuoka M. et al.: "Expression and regulation of the immediate-early gene product Arc in the accessory olfactory bulb after mating in male rat"Neuroscience. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matusmura K et al.: "Coinduction of microsomal-type PgE synthase with COX-2 in brain endothelial cells ; its possible parthophysiological role during infection and inflammation"Current Medical Chemistry. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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