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Development of method to make oocyte from male germ cells

Research Project

Project/Area Number 13480279
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionGunma University

Principal Investigator

HATADA Izuho  Gene Research Center, Associate Proffessor, 遺伝子実験施設, 助教授 (50212147)

Co-Investigator(Kenkyū-buntansha) OBATA Yayoi  Gene Research Center, Assistant Processor, 遺伝子実験施設, 助手 (70312907)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsimprinting / methylation / nuclear transplantation / germ cell / oocyte
Research Abstract

Genomicimprinitng refers to the differential expression of the two alleles of a gene, depending on their parental origins. In mammals, parental imprinting ensures the functional inequality of paternal and maternal genomes in the fertilized egg, and causes developmental failure of embryos produced by parthenogenesis or androgenesis. To elucidate the mechanisms of genomic imprinting, we examined whether maternal imprinit can be introduced to paternal genome in vitro.
First of all, we established in vitro system for completing maternal impriniting. Ovaries from mouse fetuses at 12.5 days post coitum were cultured for 17 days, followed by isolation of secondary follicles and further 11 days culture. During this period, maternal imprinitng was established in vitro.
Next we examined whether maternal imprinting can be introduced to the genome dermived from erased male germ cells by our system. Nuclear transplantation of nucleus from erased male germ cells into growing oocytes follwed by 10 days culture results in methylation of an imprinted gene Igf2r. This suggests that maternal imprint can be introduced to the paternal genome in vitro.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Higashimoto K, Soejima H, Yatsuki H, Joh K, Uchiyama M, Obata Y, Ono R, Wang Y, Xin Z, Zhu X, Masuko S, Ishino F, Hatada I, Jinno Y, Iwasaka T, Katsuki T, Mukai T: "Characterization and Imprinting Status of OBPH1/Obph1 Gene : Implications for an Extended Imprinting Domain in Human and Mouse"Genomics. 80・6. 575-584 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yatsuki H, Joh K, Higashimoto K, Soejima H, Arai Y, Wang Y, Hatada I, Obata Y, Morisaki H, Zhang Z, Nakagawachi T, Satoh Y, Mukai T: "Domain Regulation of Imprinting Cluster in Kip2/Lit1 Subdomain on Mouse Chromosome 7F4/F5 : Large-Scale DNA Methylation Analysis Reveals That DMR-Lit1 Is a Putative Imprinting Control Region"Genome Res. 12・12. 1860-1870 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hatada I, Kato A, Morita S, Obata Y, Nagaoka K, Sakurada A, Sato M, Horii A, Tsujimoto A, Matsubara K: "A microarray-based method for detecting methylated loci"J Hum Genet. 47・8. 448-451 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Obata Y, Kono T, Hatada I: "Gene silencing : maturation of mouse fetal germ cells in vitro"Nature. 418・6897. 497 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimoda M, Morita S, Obata Y, Sotomaru Y, Kono T, Hatada I: "Imprinting of a small nucleolar RNA gene on mouse chromosome 12"Genomics. 79・4. 483-486 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sotomaru Y, Katsuzawa Y, Hatada I, Obata Y, Sasaki H, Kono T: "Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses"J Biol Chem. 277・14. 12474-12478 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hatada I, Morita S, Obata Y, Sotomaru Y, Shimoda M, Kono T: "Identification of a new imprinted gene, rian, on mouse chromosome 12 by fluorescent differential display screening"J Biochem. 130. 187-190 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Gregory RI, Randall TE, Johnson CA, Khosla S, Hatada I. O'Neill LP, Turner BM, Feil R: "DNA methylation is linked to deacetylation of histone H3, but not H4, on the imprinted genes Snrpn and U2af1-rs1"Mol Cell Biol. 21. 5426-5436 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sotomaru Y, Kawase Y, Ueda T, Obata Y, Suzuki H, Domeki I, Hatada I, Kono T: "Disruption of imprinted expression of U2afbp-rs/U2af1-rs1 gene in mouse parthenogenetic fetuses"J Biol Chem. 27. 26694-26698 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Higashimoto K, Soejima H, Yatsuki H, Joh K, Uchiyama M, ObataY, Ono R, Wang Y, Xin Z, Zhu X. Masuko S, Ishino F, Hatada I, Jinno Y, Iwasaka T, Katsuki T, Mukai T.: "Characterization and Imprinting Status of OBPH1/Obph1 Gene : Implications for an Extended Imprinting Domain in Human and Mouse"Genomics. 80・6. 575-584 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yatsuki H, Job K, Higashimoto K, Soejima H, Arai Y, Wang Y, Hatada I, Obata Y, Morisaki H, Zhang Z, Nakagawachi T, Satoh Y, Mukai T.: "Domain Regulation of Imprinting Cluster in Kip2/Lit1 Subdomain on Mouse Chromosome 7F4/F5 : Large-Scale DNA Methylation Analysis Reveals That DMR-Lit1 is a Putative Imprinting Control Region"Genome Res.. 12・12. 1860-1870 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hatada I, Kato A, Morita S, Obata Y, Nagaoka K, Sakurada A, Sato M, Horii A, Tsujimoto A, Matsubara K.: "A microarray-based method for detecting methylated loci"J Hum Genet.. 47・8. 448-451 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Obata Y, Kono T, Hatada I.: "Gene silencing : maturation of mouse fetal germ cells in vitro"Nature. 418・6897. 497 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimoda M, Morita S, Obata Y, Sotomaru Y, Kono T, Hatada I.: "Imprinting of a small nucleolar RNA gene on mouse chromosome 12"Genomics. 79・4. 483-486 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sotomaru Y, Katsuzawa Y, Hatada I, Obata Y, Sasaki H, Kono T.: "Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses"J Biol Chem.. 277・14. 12474-12478 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hatada I. Morita S, Obata Y, Sotomaru Y, Shimoda M, Kono T: "Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses"J Biol Chem.. 277・14. 12474-12478 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Gregory RI, Randall TE, Johnson CA, Khosla S, Hatada I, O'Neill LP, Turner BM, Feil R: "DNA methylation is linked to deacetylation of histone H3, but not H4, on the imprinted genes Snrpn and U2afl-rs1"Mol Cell Biol. 21. 5426-5436 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sotomaru Y, Kawase Y, Ueda T, Obata Y, Suzuki H, Domeki I, Hatada I, Kono T: "Disruption of imprinted expression of U2afbp-rs/U2af1-rs1 gene in mouse parthenogenetic fetuses"J Biol Chem. 27. 26694-26698 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Higashimoto K, Soejima H, Yatsuki H, Joh K, Uchiyama M, Obata Y, Ono R, Wang Y, Xin Z, Zhu X, Masuko S, Ishino F, Hatada I, Jinno Y, Iwasaka T, Katsuki T, Mukai T.: "Characterization and Imprinting Status of OBPH1/Obph1 Gene : Implications for an Extended Imprinting Domain in Human and Mouse"Genomics. 80・6. 575-584 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yatsuki H, Joh K, Higashimoto K, Soejima H, Arai Y, Wang Y, Hatada I, Obata Y, Morisaki H, Zhang Z, Nakagawachi T, Satoh Y, Mukai T.: "Domain Regulation of Imprinting Cluster in Kip2/Lit1 Subdomain on Mouse Chromosome 7F4/F5 : Large-Scale DNA Methylation Analysis Reveals That DMR-Lit1 Is a Putative Imprinting Control Region"Genome Res.. 12・12. 1860-1870 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hatada I, Kato A, Morita S, Obata Y, Nagaoka K, Sakurada A, Sato M, Horii A, Tsujimoto A, Matsubara K.: "A microarray-based method for detecting methylated loci"J Hum Genet.. 47・8. 448-451 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Obata Y, Kono T, Hatada I.: "Gene silencing : maturation of mouse fetal germ cells in vitro"Nature. 418・6897. 497 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimoda M, Morita S, Obata Y, Sotomaru Y, Kono T, Hatada I.: "Imprinting of a small nucleolar RNA gene on mouse chromosome 12"Genomics. 79・4. 483-486 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sotomaru Y, Katsuzawa Y, Hatada I, Obata Y, Sasaki H, Kono T.: "Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses"J Biol Chem.. 277・14. 12474-12478 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sotomaru Y., Katsuzawa Y., Hatada I., Obata Y., Sasaki H., Kono T.: "Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparetal fetuses"J Biol Chem. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hatada I., Morita S., Obata Y., Sotomaru Y., Shimoda M., Kono T.: "Identification of a new imprinted gene, rian, on mouse chromosome 12 by fluorescent differential display screening"J Biochem. 130. 187-190 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Gregory RI., Randall TE., Johnson CA., Khosla S., Hatada I., O'Neill LP., Turner BM., Feil R.: "DNA methylation is linked to deacetylation of histone H3, but not H4, on the imprinted genes Snrpn and U2af1-rs1"Mol Cell Biol. 21. 5426-5436 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sotomaru Y., Kawase Y., Ueda T., Obata Y., Suzuki H., Domeki I., Hatada I., Kono T.: "Disruption of imprinted expression of U2afbp-rs/U2af1-rs1 gene in mouse parthenogenetic fetuses"J Biol Chem. 27. 26694-26698 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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