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Establishment of human tumor xenotransplantation models as a pre-clinical assisting tool for translational research

Research Project

Project/Area Number 13480283
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionCentral Institute for Experimental Animals

Principal Investigator

OHNISHI Yasuyuki  Cent.Inst.Exp.Anim., Lab.Oncology, Head, 腫瘍研究室, 室長 (70201382)

Co-Investigator(Kenkyū-buntansha) TOMISAWA Masashi  Cent.Inst.Exp.Anim., Res.Project Ctr., Researcher, 研究プロジェクトセンター, 研究員 (10321663)
SUEMIZU Hiroshi  Cent.Inst.Exp.Anim., Res.Project Ctr., Researcher, 研究プロジェクトセンター, 研究員 (40332209)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordsxenograft / human tumor / nude mouse / microarray / ant-cancer drug / eresistance / htranslational research / personalized chemotherapy
Research Abstract

In the recent strategy for treatment of cancer disease, concepts such as the order-made treatment and the target-oriented therapy are arising in consideration. To translate the basic research results based on these concepts, pre-clinical in vivo models to evaluate such treatment strategies are very important. We have been establishing human tumor xenografts, and we maintain over 600 lines of various organ tumors from human origin. In this study, we analyzed gene expression profiles and chemosensitivities of those human tumor xenografts. We used cDNA microarray representing 23,040 genes to analyze expression profiles in a panel of 85 tumor xenografts derived from human breast, lung, stomach, colon, pancreas, and other four organs. We also examine chemosensitivities for these tumors on 9 antitumor drugs including CDDP, mitomycin C,5-FU. Comparison of the gene expression profiles and chemosensitivities of the tumors identified that 1,578 genes whose expression levels correlated significantly with drug effectiveness; 333 of those genes showed significant correlation with two or more drugs. These data should contribute useful information for identifying predictive markers for drug sensitivity that may eventually provide personalized chemotherapy for individual patients.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Tomii, Y.: "Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells"Int.J.Oncol.. 20. 339-342 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zembutsu H.: "Genome-wide cDNA microarray screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs."Cancer Res.. 62. 518-527 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zembutsu H.: "Gene expression profiles of human tumor xenografts in nude mice treated orally with the EGFR tyrosine kinase inhibitor ZD1839."Int.J.Oncol.. 23. 29-39 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tokunaga, T.: "Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line."Int.J.Oncol.. 21. 1027-1032 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Naruke, M.: "Interleukin-10 expression is correlated with growth fraction in human non small cell lung cancer xenografts."Int.J.Oncol.. 18. 1213-1217 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyakawa, Y.: "Establishment of a new model of human multiple myeloma using NOD/SCID/γc^<null>(NOG) mice"Biochem.Biophysical Res.Commun.. 313. 258-262 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomii, Y., et al.: "Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells"Int.J.Oncol.. 20. 339-342 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zembutsu H., et al.: "M.Genomewide cDNA microarray screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs."Cancer Res.. 62. 518-527 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zembutsu H., et al.: "Gene-expression profiles of human tumor xenogmfts in nude mice treated orally with the EGFR tyrosine kinase inhibitor ZD1839."Int.J.Oncol.. 23. 29-39 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tokunaga, T, et al.: "Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line."Int.J.Oncol.. 21. 1027-1032 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Naruke, M., et al.: "Interleukin-10 expression is correlated with growth fraction in human non-small cell lung cancer xenografts."Int.J.Oncol.. 18. 1212-1217 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyakawa, Y., et al.: "Establishment of a new model of human multiple myeloma using NOD/SCID/γ cnull (NOG) mice."Biochem.Biophysical Res.Commun.. 313. 258-262 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tomii, Y.: "Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells"Int.J.Oncol.. 20. 339-342 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zembutsu H.: "Genome-wide cDNA microarray screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs."Cancer Res.. 62. 518-527 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zembutsu H.: "Gene-expression profiles of human tumor xenografts in nude mice treated orally with the EGFR tyrosine kinase inhibitor ZD1839."Int.J.Oncol.. 23. 29-39 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tokunaga, T: "Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line."Int.J.Oncol. 21. 1027-1032 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Naruke, M.: "Interleukin-10 expression is correlated with growth fraction in human non-small cell lung cancer xenografts."Int.J.Oncol.. 18. 1213-1217 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyakawa, Y: "Establishment of a new model of human multiple myeloma using NOD/SCID/γc^<null> (NOG) mice"Biochem.Biophysical Res.Commun.. 313. 258-262 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tomii, Y.: "Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells"Int.J.Oncol.. 20. 339-342 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Zembutsu H.: "Genome-wide cDNA microatray screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs"Cancer Res.. 62. 518-527 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suemizu, H.: "Two-color allele-specific polymerase chain reaction (PCR-SSP) assay of the leptin receptor gene (Leprdb) for genotyping mouse diabetes mutation"Exp.Anim.. 50. 435-439 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Goto, K.: "Contamination of transplantable human tumor-bearing lines by Helicobacter hepaticus and its elimination"J.Clin.Microbiology. 39. 3703-3704 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Naruke, M.: "Interleukin-10 expression is correlated with growth fraction in human non-small cell lung cancer xenografts"Int.J.Oncol.. 18. 1213-1217 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tokunaga, T.: "Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line"Int.J.Oncol.. 163. 1027-1032 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomii, Y.: "Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells"Int.J.Oncol. 20. 339-342 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Zembutsu H.: "Genome-wide cDNA micro array screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs"Cancer Res.. 62. 518-527 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suemizu, H.: "Two-color allele-specific polymerase chain reaction (PCR-SSP) assay of the leptin receptor gene (Leprdb) for genotyping mouse diabetes mutation"Exp.Anim. 50. 435-439 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Goto, K.: "Contamination of transplantable human tumor-bearing lines by Helicobacter hepaticus and its elimination"J.Clin.Microbiology. 39. 3703-3704 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Naruke, M.: "Interleukin-10 expression is correlated with growth fraction in human non-small cell lung cancer xenografts"Int.J.Oncol. 18. 1213-1217 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Birumachi, J.: "Diesel exhaust-induced airway hyperresponsiveness in c-Ha-ras transgenic mice"Toxicology. 163. 145-152 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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