| Project/Area Number |
13480284
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Tohoku University (2002-2003)
Hokkaido University (2001) |
|---|
Principal Investigator |
WADA Shigeo Tohoku University, Graduate School of Engineering, Professor, 大学院・工学研究科, 助教授 (70240546)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Ryo Hiroshima University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (60153657)
YAMAGUCHI Takami Tohoku University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (30101843)
TSUBOTA Ken-ichi Tohoku University, Graduate School of Engineering, Res. Associate, 大学院・工学研究科, 助手 (10344045)
ISHII Katsuhiro Hokkaido University, Research Institute for Electronic Science, Research Associate, 電子科学研究所, 助手 (30311517)
IWAI Toshiaki Hokkaido University, Research Institute for Electronic Science, Associate Professor, 電子科学研究所, 助教授 (80183193)
|
|---|
| Project Period (FY) |
2001 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2002: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥10,000,000 (Direct Cost: ¥10,000,000)
|
|---|
| Keywords | Computational Biomechanics / Blood Flow / Red blood cell / Dynamic Light Scattering / Spring Network Model / Energy principle / Particle Method / Hemolysis / レオロジー / 連銭 / 形状 / 変形 / 動的散乱 / 凝集 |
|---|
| Research Abstract |
In order to establish the fundamental approach in the development of hemolysis simulator, we have carried out experimental and computational analyses of the mechanical behavior of elastic red blood cells (RBCs) in blood flow. In the experimental studies, we have investigated the way using dynamic light scattering to measure the deformation and aggregation of RBCs under shear flow in cone-plate rotational viscometer. It was shown that the temporal autocorrelation function on the intensity basis is the square of the sum of the autocorrelation functions on the amplitude basis of light scattering from particles and the dynamic speckle caused by the rough surface of the rotating plate in the viscometer. By using this method, the relationship between the RBC behavior in shear flow and the viscosity of the whole blood could be clarified. In the computational studies, first, we have constructed a spring network model of RBC membrane which represents the various shape change of RBC in blood flow based on the energy principle and showed that a spherical RBC transformed itself into a biconcave discoid shape, a cupped shape and a spiculate shape when the volume in decreased. Secondly, we have demonstrated the collision and aggregation of elastic RBCs in blood flow by assuming a potential functional each node of the elastic network model, which expresses the attracting and repulsive forces between neighboring membranes. Thirdly and finally, we have proposed particle method for computer simulation of bloodflow by combining the elastic RBC model and a particle model for plasma flow, and analyzed the mechanical behavior of RBCs in various blood flow. It is possible to evaluate the hemolysis from the mechanical behavior of RBC in blood flow by combining these experimental and computational methods.
|
