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Effect of Tumor-Specific Leukocyte Behavior on Tumor Growth

Research Project

Project/Area Number 13480287
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionUniversity of Tsukuba

Principal Investigator

OHSHIMA Norio  University of Tsukuba, Institute of Basic Medical Sciences, Professor, 基礎医学系, 教授 (50015971)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2002: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2001: ¥7,700,000 (Direct Cost: ¥7,700,000)
Keywordstumor microcirculation / hemodynamics / angiogenesis / nitric oxide / confocal laser-scanning microscope / intravital microscope / leukocyte
Research Abstract

Using an experimental tumor model developed by the authors, intravital microscopic observations of the angiogenic processes in the mesenteric microvasculature and the hemodynamics of the newly formed microvessels were performed. In this model, tumor growth accompanying a marked angiogenesis was microscopically observable for up to 10 days. The length of the microvessels showed a significant increase in the tumor-bearing mesentery. In the newly formed tumor vessels, rolling and adherence of leukocytes in venules were both attenuated as compared with those in the normal mesenteric venules. To elucidate the mechanisms of these altered behavior of hemodynamics, the role of nitric oxide (NO) in regulating leukocyte dynamics in the tumor microcirculation was examined. Reduction of the baseline levels of leukocyte-endothelial cell interactions observed in the tumor venules were markedly increased by inhibition of NO synthase (NOS). These increases were neither found in the tumor-free rats nor in normal microvessels of the tumor-bearing rats. These results suggest that an excessive production of NO in the tumor tissues creates the tumor-specific microenvironment, thereby modulating leukocyte behavior in the angiogenic tumor vessels.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Chika MIYOSHI: "Tumor-derived Nitric Oxide Attenuates Leukocyte-Endothelial Cell Interactions in the Angiogenic Tumor Vessels"Microcirculation Annual. 18. 107-108 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Eri TAGUCHI: "The Relationship between Tumor-derived Nitric Oxide and Tumor Growth"Microcirculation Annual. 18. 109-110 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Vascular Endothelial Growth Factor (VEGE) Expression Regulates Angiogenic Growth of Microvasculature in a Peritoneal Disseminated Colon Carcinoma"Biorheology. 38. 450 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Effects of Nitric Oxide on Leukocyte Behavior in the Angiogenic Tumor Microvessels"Tissue Engineering for Therapeutic Use. 6. 105-109 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lazaro A.LOAIZA: "Vasodilatation of Muscle Microvessels Induced by Somatic Afferent Stimulation Is Mediated by Calcitonin Gene-related Peptide Release in the Rat"Neuroscience Letters. 333. 136-140 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lazaro A.LOAIZA: "Electro-acupuncture Stimulation to Muscle Afferents in Anesthetized Rats Modulates the Blood Flow to the Knee Joint through Autonomic Reflexes and Nitric Oxide"Autonomic Neuroscience ; Basic and Clinicals. 97. 103-109 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinjiro YAMAGUCHI: "Effects of Electrical Stimulation of the Dorsal Skin on Systemic and Mesenteric Microvascular Hemodynamics in Anesthetized Rats"Japanese Journal of Physiology. 18. 257-265 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Nitric Oxide Attenuates Leukocyte-Endothelial Cell Interactions in Tumor Microessels"Microcirculation Annual. 17. 143-144 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 三好千香: "腫瘍の増殖過程における血管新生:血管内皮細胞増殖因子(VEGF)の関与"B&R(日本バイオレオロジー学会誌). 14. 30-39 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Vascular Endothelial Growth Factor (VEGF) Expression Regulates Angiogenesis Accompanying Tumor Growth in a Peritoneal Disseminated Tumor Model"In Vivo. 15. 233-238 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Leukocyte-Endothelial Cell Interactions in Tumor Microvessels (Meeting abstract)"Japanese Journal of Physiology. 51. S55 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinjiro YAMAGUCHI: "Somatosensory Nociceptive Mechanical Stimulation Modulates Systemic and Mesenteric Microvascular Hemodynamics in Anesthetized Rats"Autonomic Neuroscience ; Basic and Clinicals. 88. 160-166 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Tumor-derived Nitric Oxide Attenuates Leukocyte-Endothelial Cell Interactions in the Angiogenic Tumor Vessels"Microcirculation Annual. 18. 107-108 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Eri TAGUCHI: "The Relationship between Tumor-derived Nitric Oxide and Tumor Growth"Microcirculation Annual. 18. 109-110 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Effect of Nitric Oxide on Leukocyte Behavior in the Angiogenic Tumor Microvessels"Tissue Engineering for Therapeutic Use (Amsterdam : Elsevier Science). 6. 105-109 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lazaro A. LOAIZA: "Vasodilatation of Muscle Microvessels Induced by Somatic Afferent Stimulation Is Mediated by Calcitonin Gene-related Peptide Release in the Rat"Neuroscience Letters. 333. 136-140 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lazaro A. LOAIZA: "Electro-acupuncture Stimulation to Muscle Afferents in Anesthetized Rats Modulates the Blood Flow to the Knee Joint through Autonomic Reflexes and Nitric Oxide"Autonomic Neuroscience ; Basic and Clinicals. 97. 103-109 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinjiro YAMAGUCHI: "Effects of Electrical Stimulation of the Dorsal Skin on Systemic and Mesenteric Microvascular Hemodynamics in Anesthetized Rats"Japanese Journal of Physiology. 52. 257-265 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Nitric Oxide Attenuates Leukocyte-Endothelial Cell Interactions in Tumor Microvessels"Microcirculation Annual. 17. 143-144 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Vascular Endothelial Growth Factor (VEGF) Expression Regulates Angiogenesis Accompanying Tumor Growth in a Peritoneal Disseminated Tumor Model"In Vivo. 15. 233-238 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Nitric Oxide Attenates Leukocyte-Endothelial Cell Interactions in Tumor Microvessels"Japanese Journal of Physiology. 51. S55 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MIYOSHI: "Nitric Oxide Attenuates Leukocyte-Endothelial Cell Interactions in a Peritoneal Disseminated Tumor Model"Proceedings of 7^<th> World Congress for Microcirculation, Bologna : Monduzzi Editore (ISBN 88-323-1819-9). 457-459 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinjiro YAMAGUCHI: "Somatosensory Nociceptive Mechanical Stimulation Modulates Systemic and Mesenteric Microvascular Hemodynamics in Anesthetized Rats"Autonomic Neuroscience ; Basic and Clinicals. 88. 160-166 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chika MTYOSHI: "Tumor-derived Nitric Oxide Attenuates Leukocyte-Endothelial Cell Interactions in the Angiogenic Tumor Vessels"Microcirculation Annual. 18. 107-108 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Eri TAGUCHI: "The Relationship Between Tumor-derived Nitric Oxide and Tumor Growth"Microcirculation Annual. 18. 109-110 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chika MIYOSHI: "Vascular Endothelial Growth Factor (VEGF) Expression Regulates Angiogenic Growth of Microvasculature in a Peritoneal Disseminated Colon Carcinoma"Biorheology. 38. 450 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chika MIYOSHI: "Effects of Nitric Oxide on Leukocyte Behavior in the Angiogenic Tumor Microvessels"Tissue Engineering for Therapeutic Use. 6. 105-109 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Lazaro A.LOAIZA: "Vasodilatation of Muscle Microvessels Induced by Somatic Afferent Stimulation Is Mediated by Calcitonin Gene-related Peptide Release in the Rat"Neuroscience Letters. 333. 136-140 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] LazaroA. LOAIZA: "Electro-acupuncture Stimulation to Muscle Afferents in Anesthetized Rats Modulates the Blood Flow to the Knee Joint Through Autonomic Reflexes and Nitric Oxide"Autonomic Neuroscience ; Basic and Clinicals. 97. 103-109 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinjiro YAMAGUCHI: "Effects of Electrical Stimulation of the Dorsal Skin on Systemic and Mesenteric Microvascular Hemodynamics in Anesthetized Rats"Japanese Journal of Physiology. 18. 257-265 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chika MIYOSHI: "Nitric Oxide Attenuates Leukocyte-Endothelial Cell Interactions in Tumor Microessels"Microcirculation Annual. 17. 143-144 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 三好千香: "腫瘍の増殖過程における血管新生:血管内皮細胞増殖因子(VEGF)の関与"B&R(日本バイオレオロジー学会誌). 14. 30-39 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chika MIYOSHI: "Vascular Endothelial Growth Factor (VEGF) Expression Regulates Angiogenesis Accompanying Tumor Growth in a Peritoneal Disseminated Tumor Model"In Vivo. 15. 233-238 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chika MTYOSHI: "Leukocyte-Endothelial Cell Interactions in Tumor Microvessels"Japanese Journal of Physiology. 51. S55 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shinjiro YAMAGUCHI: "Somatosensory Nociceptive Mechanical Stimulation Modulates Systemic and Mesenteric Microvascular Hemodynamics in Anesthetized Rats"Autonomic Neuroscience ; Basic and Clinicals. 88. 160-166 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chika MIYOSHI: "Vascular Endothelial Growth factor (VEGF) Expression Regulates Angiogenesis Accompanying Tumor Growth in a Peritoneal Disseminated Tumor Model"in vivo. 15. 233-238 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Chika MIYOSHI: "Nitric Oxide Attenuates Leukocytes-Endothelial Cell Interactions in Tumor Microvessels"Microcirculation Annual. 17. 143-144 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Chika MIYOSHI: "Vascular Endothelial Growth Factor (VEGF) Expression Regulates Angiogenic Growth of Microvasculature in a Peritoneal Disseminated Colon Carcinoma"Biorheology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Chika MIYOSHI: "Nitric Oxide Attenates Leukocyte-Endothelial Cell Interactions in Tumor Microvessels"Japanese Journal of Physiology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 三好千香: "腫瘍の増殖過程における血管新生: 血管内皮細胞増殖因子(VEGF)の関与"B&R(日本バイオレオロジー学会誌). 14・5. 30-39 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Chika MIYOSHI: "Effects of Nitric Oxide on Leukocyte Behavior in the Angiogenic Tumor Microvessels"Tissue Engineering for Therapeutic Use 6. (in press).

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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