• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Research of bone regeneration using cultured cells under mechanical stress

Research Project

Project/Area Number 13480292
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionNagoya University

Principal Investigator

HATA Ken-ichiro  Nagoya University, University Hospital, Associate Professor, 医学部附属病院, 助教授 (80293710)

Co-Investigator(Kenkyū-buntansha) TOSHIHIRO Kasuga  Nagoya Institute of Technology, Department of Engineering, Associate Professor, 工学部, 助教授 (30233729)
OKADA Kunihiko  Nagoya University, School of Medicine, Research Associate, 医学部, 助手 (20345911)
UEDA Minoru  Nagoya University, Graduate school of Medicine, Professor, 大学院・医学系研究科, 教授 (00151803)
日比野 祥敬  名古屋大学, 医学部, 助手 (90324441)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥7,900,000 (Direct Cost: ¥7,900,000)
Keywordsmechanical stress / LIPUS / MSC(mesenchvmal stem cell) / cyclic stretch / artificial bone / oxidative stress / bone regeneration / mechano transduction / 多血小板血漿 / 伸展ストレス / 超音波 / 骨誘導 / ALP
Research Abstract

In clinical studies and animal models, low-intensity ultrasound (US) promotes fracture repair and increases the mechanical strength. US also promotes bone and cartilage healing by increasing bone marker and glycosaminoglycan synthesis of chondrocytes. As mesenchymal stem cells (MSCs) have the ability to differentiate into osteoblasts and chondrocytes, US may promote their differentiation. Here, we evaluated the effects of US on the differentiation of MSCs toward osteoblasts or chondrocytes. When human MSCs cultured in pellets, they differentiated into osteoblasts or chondrocytes as assessed by immunostaining and western blotting. Furthermore, when low-intensity US was applied for 20 minutes everyday, osteoblast and chondrocyte differentiation was enhanced. Biochemically, ALP activity or aggrecan deposition was increased by more than 1.5 fold by treatment with induction medium, and with both induction medium and US, respectively. In contrast, cell proliferation and total protein amount appeared unaffected by these treatments. These results indicate that low-intensity US enhances induction medium-mediated differentiation of MSCs and that application of US may facilitate larger preparation of osteoblasts and chondrocytes.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Ebisawa K, Hata K.I, OkadaK, Kimata K, Ueda M, Torii S, Watanabe H.: "Ultrasound Enhances TGF-b Mediated Chondrocyte Differentiation of Human Mesenchymal Stem Cells."Tissue engineering. 10,3(In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kasuga T, Maeda H, Kato K, Nogami M, Hata K, M. Ueda: "Preparation of poly(lactic acid) composites containing calcium carbonate (vaterite)"Biomaterials. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada Y, Boo J.S, Ozawa R, Nagasaka T, Okazaki Y, Hata K, Ueda M: "Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold."J.Cranio-fac.Surg.. 31. 27-33 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeda H, Kasuga T., Nogami M., Hibino Y., Hata K., Ueda M., Ota Y.: "Preparation of Bioactive Polylactic Acid Composites Containing Calcium Carbonates"Key Engng.Mater.. 24. 163-166 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ebisawa K, Hata K.I, Okada K, Kimata K, Ueda M, Torii S, Watanabe H.: "Ultrasound Enhances TGF-b Mediated Chondrocyte Differentiation of Human Mesenchymal Stem Cells."Tissue engineering. 10,3(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kasuga T, Maeda H, Kato K, Nogami M, Hata K, M.Ueda: "Preparation of poly(lactic acid) composites containing calcium carbonate (vaterite)"Biomaterials. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamada Y, Boo J.S, Ozawa R, Nagasaka T, Okazaki Y, Hata K, Ueda M: "Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold."J.Cranio-fac.Surg.. 31. 27-33 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeda H, Kasuga T., Nogami M., Hibino Y., Hata K., Ueda M., Ota Y.: "Preparation of Bioactive Polylactic Acid Composites Containing Calcium Carbonates"Key Engng.Mater.. 240-242. 163-166 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ebisawa K, Hata K.I, Okada K, Kimata K, Ueda M, Torii S, Watanabe H: "Ultrasound Enhances TGF-b Mediated Chondrocyte Differentiation of Human Mesenchymal Stem Cells"Tissue engineering. 10, 3. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamada Y, Boo J.S, Ozawa R, Nagasaka T, Okazaki Y, Hata K, Ueda M: "Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold"J.Cranio-fac.Surg.. 31. 27-33 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Maeda H, Kasuga T., Nogami M., Hibino Y., Hata K., Ueda M., Ota Y.: "Preparation of Bioactive Polylactic Acid Composites Containing Calcium Carbonates"Key Engng.Mater.. 24. 163-166 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoichi Yamada et al.: "Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold"Journal of Cranio-Maxillofacial Surgery. 31. 27-33 (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi