| Project/Area Number |
13554029
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
分離・精製・検出法
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| Research Institution | Tohoku University |
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Principal Investigator |
TERAMAE Norio Graduate School of Science. Tohoku University, Professor, 大学院・理学研究科, 教授 (70114569)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Tatsuya School of Lila Science, Tokyo University of Pharmacy and Life Science, Associate Professor, 生命科学部, 助教授 (30261548)
YAMAGUCHI Akira Graduate School of Science, Tohoku University, Assistant Professor, 大学院・理学研究科, 助手 (10359531)
NISHIZAWA Seiichi Graduate School of Science, Tohoku University. Assistant Professor, 大学院・理学研究科, 助手 (40281969)
KAGAWA Yasuhiro Toray Research Center, Inc., Senior Researcher, 生物化学研究部, 主任研究員
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2002: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2001: ¥9,500,000 (Direct Cost: ¥9,500,000)
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| Keywords | chemical sensor / nucleobase / fluorescence / self-assembly / molecular recognition / bio-marker / creatinine / abasic site / グアニン / テロメア / 検出 / 自己会合 / オクタマー |
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| Research Abstract |
1. Template-assisted preferential formation of syn photodimer in a pyrophosphate-induced self-assembly of a thymine-functionalized isothiouronium receptor: The effect of anion-templation is investigated for the photodimerization of a thymine-functionalized isothiouronium receptor. The receptor forms a photodimer at the thymine moiety is methanol upon the UV-irradiation, while the isothiouronium moiety works as on oxoanion binding site via o two-point hydrogen-bonding motif. As compared to the case of free receptor, the presence of pyrophosphate (PPi) resulted is the preferential formation of the cis-syn type photodimer, which would be desirable to recognize the templated PPi.
Fluorescence Detection of Creatinine by a Hydrogen-Bonded Guanosine Octamer: We report the design and characterization of guanosine-based octamers held together by twenty-four hydrogen bonds and stacking interaction, capable of fluorescent detection of a clinically important substrate. The octamers consist of two c
… More
yclic guanosine-quartets, an formed by Hoogsteen base-pairing, and also by hydrogen bonds two quartets are coupled together so as to stack with a counterclockwise rotation. When creatinine binds to guanosine of the octamer by three hydrogen bonds similar to Watson-Crick base-pairing, the octamer labelled with pyrene dissociates to give a monomeric form accompanied by the change in visible fluorescence from green to purple.
Use of A basic Site Containing DNA Strands for Nucleobase Recognition in Water: Nucleobase recognition in water is successfully achieved by the use of an abasic site (AP site) as the molecular recognition field. We intentionally constructed the AP site is DNA duplex so as to orient the AP site toward a target nucleobase, and examine the complexation of 2-amino-7-methylnaphthyridine (AMND) with nucleobases at the AP site. AMND is found to selectively bind to cytosine base (K_<11>>10^6M^1) accompanied by remarkable quenching of its fluorescence, and is available for selective and visible detection of a single-base alternation related to the cytosine base. Less
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