| Project/Area Number |
13555147
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OHGAKI Shinichiro Graduate School of Engineering, Professor, 大学院・工学系研究科, 教授 (20005549)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Hiroyuki Graduate School of Frontier Science, Lectuer, 大学院・新領域創成科学研究科, 講師 (00302779)
OTAKI Masahiro Ochanomizu University, Graduate School of Humanities and Sciences, Associate Professor, 大学院・人間文化研究科, 助教授 (70272367)
MITANI Hiroshi Graduate School of Frontier Sciences, Associate Professor, 大学院・新領域創成科学研究科, 助教授 (70181922)
SUZUKI Yutaka Public Works Research Institute, Material and Geotechnical Engineering group, Senior Researcher, 材料地盤研究グループ, 上席研究員 (20231376)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 2002: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2001: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | ultraviolet inactivation / photoreactivation / Escherichia coli / Crvptosporidium parvum / Legionella pneumophila / low-pregsure UV lamp / medium-pressure UV lamp / ESS法 / Escherichia coli / Crystosporidium parvum / ピリミジン二量体 / 生残率測定法 / 紫外線感受性 |
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| Research Abstract |
Ultraviolet inactivation and photoreactivation of Escherichia coli, Cryptosporidium parvum and Legionella pneumophila were compared using a low-pressure UV lam (LP ; 254nm) or a medium-pressure UV lamp (MP ; 220nm-580nm). Filtration of the MP emissions was also carried out to investigate the effect of wavelengths on the characteristics of inactivation and photoreactivation of microorganisms. An endonuclease sensitive site (ESS) assay was applied to determine the number of UV-induced pyrimidine dimers in the genomic DNA, while a conventional cultivation or infectivity assay was also used to investigate the viability of microorganisms.
This research indicated that (l) C. parvum can be effectively inactivated by UV without photoreactivation, (2) MP lamp can repress photoreactivation of E. coli probably by affecting photolyase (3) L. pneumophila performs evident photoreactivation either after LP or MP lamp inactivation. These conclusions may encourage a development of UV systems with high disinfection efficiency at low photoreactivation risk.
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