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Development of an in vivo real-time measurement system of nitric oxide and superoxide and their clinical applications

Research Project

Project/Area Number 13555228
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物・生体工学
Research InstitutionKawasaki College of Allied Health Professions

Principal Investigator

MOCHIZUKI Seiichi  Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Associate Professor, 臨床工学科, 助教授 (60259596)

Co-Investigator(Kenkyū-buntansha) KATAOKA Noriyuki  Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Assistant Professor, 臨床工学科, 講師 (20250681)
HIRAMATSU Osamu  Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Assistant Professor, 臨床工学科, 講師 (50208849)
GOTO Masami  Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Professor, 臨床工学科, 教授 (50148699)
YOSHIDA Kiyoshi  Kawasaki Medical School, Div.of Cardiovascular Medicine, Professor, 医学部, 教授 (60322583)
MIYASAKA Takehiro  Okayama University Medical School, Dept.of Cardiovascular Physiology, Research Associate, 大学院・医歯学総合研究科, 助手 (60308195)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥10,100,000 (Direct Cost: ¥10,100,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsnitric oxide (NO) / superoxide / sensor / catheter / endothelial function / superoxide dismutase (SOD) / 酸化窒素(NO) / スーパーオキサイド / 冠状静脈洞 / センサー / in vivo計測
Research Abstract

Development of a catheter-type NO sensor : First, we evaluated the basic performance of an in vivo NO sensor. Using a NO gas-saturated pure water, the sensor showed a sensitivity of about 580 pA/nM-NO. The sensor also demonstrated high selectivity to NO. When nitroglycerin was infused, plasma NO level in the abdominal aorta of an anesthetized rat increased to some nM level. Secondly, a proto-type catheter-type NO sensor was developed by encasing the in vivo NO sensor in a 4F catheter. The sensor was inserted into the thoracic aorta of an anesthetized dog. Plasma NO level increased transiently by acetylcholine (ACh) infusion. After inhibition of NO synthesis by L-NMMA, the ACh-induced increase in plasma NO level was significantly lower than that before L-NMMA. Finally, we have developed a catheter-type NO sensor for NO measurement in the proximal vessels. A soft tip was fixed at the edge of the detection tip. The sensor was inserted into the coronary sinus of an anesthetized dog. Intra-coronary infusion of ACh increased plasma NO level up to 5 nM. In conclusion, the newly developed catheter-type NO sensors demonstrated satisfying performance and would be applicable to clinical evaluation of endothelial function.
Development of a superoxide sensor : A superoxide was prepared by fixing SOD on a Pt wire with some chemical modification. The sensor was located in a flow-cell-type measurement system. Isolated organs (heart, liver, and kidney) were loaded into the system, and superoxide level released from these organs could be measured successfully.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Mochizuki S, et al.: "Effect of dietary control on plasma nitrate level and estimation of basal systemic nitric oxide production rate in humans"Heart.Vessels. 15. 274-279 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mochizuki S, et al.: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovasc.Eng.Int.J.. 1. 85-91 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mochizuki S, et al.: "Evaluation of basic performance and applicability of a newly developed in vivo nitric oxide sensor"Physiol.Meas.. 23. 261-268 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyasaka T, et al.: "Development of a contamination free 6 valve injector inline monitoring system for endotoxin measurement in dialysate"ASAIO J.. 48. 389-393 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Endo K, et al.: "Development of a superoxide sensor by immobilization of superoxide dismutase"Sensors Actuator. 83. 30-34 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 望月精一: "冠循環のバイオメカニクス(梶谷文彦編著):ノート(メカノストレスとNO、EDHF、CO、PGI_2)"コロナ社. 222 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Effect of dietary control on plasma nitrate level and estimation of basal systemic nitric oxide production rate in humans"Heart.Vessels. 15(6). 274-279 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovasc.Eng.Int.J.. 1(2). 85-91 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Evaluation of basic performance and applicability of a newly developed in vivo nitric oxide sensor"Physiol.Meas.. 23(2). 261-268 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Toyotaka Yada, et al.: "Roles of ADMA, BH4, oxidative stress as risk factors and antioxidant effect of an HMG-CoA reductase inhibitor in insulin-resistant Zucker fatty rats"Journal of Japanese College of Angiology. 42(5). 297-300 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kosuke Endo, et al.: "Development of a superoxide sensor by immobilization of superoxide dismutase"Sensors Actuator. 83. 30-34 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takehiro Miyasaka, et al.: "Development of a contamination free 6 valve injector inline monitoring system for endotoxin measurement in dialysate"ASAIO J.. 48. 389-393 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Measurement of acetylcholine-induced endothelium-derived nitric oxide in aorta using a newly developed catheter-type nitric oxide sensor"Biochem.Biophys.Res.Commun.. 306. 505-508 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masayo Hayama, Takehiro Miyasaka, Seiichi Mochizuki, et al.: "Optimum dialysis membrane for endotoxin blocking"J.Memb.Sci.. 219. 15-25 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release"Am.J.Physiol.Heart Circ.Physiol.. 285. H722-H726 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Seiichi Mochizuki, et al.: "Measurement of acetylcholine-induced endothelium-derived nitric oxide in aorta using a newly developed catheter-type nitric oxide sensor"Biochemical and Biophysical Research Communications. 306. 505-508 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Masayo Hayama, Takehiro Miyasaka, Seiichi Mochizuki, et al.: "Optimum dialysis membrane for endotoxin blocking"Journal of Membrane Science. 219. 15-25 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Seiichi Mochizuki, et al.: "Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release"American Journal of Physiology. 285. H722-H726 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 遠藤恒介, 宮坂武寛, 望月精一 他: "スーパーオキサイドセンサ"Medical Science Digest. 29・14. 20-23 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 氷見直之, 宮坂武寛, 望月精一 他: "スーパーオキサイド産生量の正常と病態ラットにおける臓器間差異の解析"信学技報. MBE2003-50. 43-46 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 望月精一他: "血液透析に関連した病態におけるNO・酸化ストレスの関連因子の計測・評価"腎臓. 26・2. 172-176 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 矢田豊隆: "内因性NO合成阻害因子と酸化ストレス起因性血管内皮障害の発症メカニズムとHMG-CoA reductase inhibitor長期投与の効果"脈管学. 42・5. 297-300 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kosuke Endo: "Development of a superoxide sensor by immobilization of superoxide dismutase"Sensors and Actuator. 83. 30-34 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takehiro Miyasaka: "Development of a contamination free 6 valve injector inline monitoring system for endotoxin measurement in"ASAIO Journal. 48. 389-393 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 宮坂武寛: "生体計測用スーパーオキサイドセンサ"医工学治療. 14・3. 158-161 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Seiichi Mochizuki: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovascular Engineering : An International Journal. 1・2. 85-91 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seiichi Mochizuki: "Effect of dietary control on plasma nitrate level and estimation of basal systemic nitric oxide production rate in humans"Heart and Vessels. 15・6. 274-279 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seiichi Mochizuki: "Evaluation of basic performance and applicability of a newly developed in vivo nitric oxide sensor"Physiological Measurement. 23・2. 261-268 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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