| Project/Area Number |
13555231
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
工業分析化学
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| Research Institution | University of Yamanashi |
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Principal Investigator |
KIBA Nobutoshi UNIVERSITY OF YAMANASHI, INTERDISCIPLINARY GRADUATE SCHOOL OF MEDICINE AND ENGINEERING, PROFESSOR, 大学院・医学工学総合研究部, 教授 (20020505)
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| Co-Investigator(Kenkyū-buntansha) |
URA Nobuo SOMA OPTICS LIT., RESEARCH CENTER, RESEARCH DIRECTOR, 研究担当取締役
TANI Kazue UNIVERSITY OF YAMANASHI, INTERDISCIPLINARY GRADUATE SCHOOL OF MEDICINE AND ENGINEERING, ASSOCIATE PROFESSOR, 大学院・医学工学総合研究部, 助教授 (60115318)
YAMANE Takeshi UNIVERSITY OF YAMANASHI, FACULTY OF EDUCATION AND HUMAN SCIENCES, PROFESSOR, 教育人間科学部, 教授 (10020405)
YONEZAWA Eiichi FUJI ELECTRIC CORPORATE RESEARCH AND DEVELOPMENT, FUCTIONAL DEVICE LABORATORY, RESEARCH MANAGER, 機能デバイス研究所, 研究マネージャ
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | FLOW SYSTEM / MICROFLUID SYSTEM / CHEMILUMINESCENCE / DETECTOR / MINIATURIZED / HYDROGEN PEROXIDE / HIGH THROUGHPUT / ENZYME / 化学発光検出 / 検出器 / 小型化 / 過酸化水素 / ハイスループット分析 / 固定化酵素 / マイクロTAS / 化学発光 / 科学発光 |
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| Research Abstract |
A MICROMACHINED FLOW CELL (OVERALL SIZE; 25x25x1 mm^3) WAS DESIGNED FOR HIGH THROUGHPUT ANALYSIS. THE FLOW CELL CONSISTED OF A SILICON BOTTOM CHIP (0.5mm THICK) WHICH HAD A SPIRAL CHANNEL (20 TURNS), TWO PIERCED HOLES (1mm DIAMETER), AND A GLASS TOP PLATE (0.5mm THICK). THE CHANNEL (50cm LENGTH, 150μm WIDTH, 20μm DEPTH, 150μm SPACING BETWEEN CHANNELS) WAS PRODUCED BY ECHING. TW0 HOLES SERVE AS THE FLUID INLET (CENTER) AND OUTLET (SIDE). THE CHIP WAS PLACED IN FRONT OF A WINDOW OF A PHOTOMULTIPLIER TUBE. ALSO, TRANSPARENT FUSED SILICA CAPILLARY (50μm I. D., 375μm O. D.) WHICH IS COATED WITH TEFLON AF WAS USED FOR MORE RAPID ANALYSIS. THE CHIP AND CAPILLARY WERE USED AS FLOW CELLS IN A SINGLE-LINE CHEMILUMINOMETRIC FLOW INJECTION SYSTEM. PEROXIDASE (EC 1.11.1.7) WAS COVALENTLY IMMOBILIZED ON THE INNER SURFACE OF THE CHANNEL. THE SAMPLE VOLUME FOR ONE MEASUREMENT WAS 0.2μL. THE MAXIMUM SAMPLING RATE WAS 315/h AT A CARRIER SOLUTION FLOW RATE OF 10μl/min. A CALIBRATION GRAPH FOR H_20_2 WAS LINEAR FOR 5 nM -5μM. THE SYSTEM WITH μ-FLOW-THROUGH DETECTOR WAS APPLIED TO THE DETERMINATIONS OF H_20_2 IN ENVIROMENTAL SAMPLES AND OF QXIDASE SUBSTRATES IN BIOCHEMICAL SAMPLES.
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