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Development of a method for a quick measurement of the root capacity of water uptake and solute transport

Research Project

Project/Area Number 13556005
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 作物学
Research InstitutionNational University Corporation Tokyo University of Agriculture and Technology

Principal Investigator

HIRASAWA Tadashi  National University Corporation Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Professor, 大学院・共生科学技術研究部, 教授 (30015119)

Co-Investigator(Kenkyū-buntansha) OOKAWA Taiichiro  National University Corporation Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Associate Professor, 大学院・共生科学技術研究部, 助教授 (80213643)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsHydraulic conductivity / NaCl / Reflection coefficient / Root / Root pressure probe / Solute transport / Water uptake / Xylem pressure / NaClの吸収 / NaClの透過係数 / NaClの反射係数 / 根圧 / トウモロコシ / ヤエヤマヒルギ / オオムギ / 冠根 / 水稲 / 耐塩性 / 定常状態 / 根の吸水部位 / 非定常状態 / 水の通導抵抗 / 水耕液のNaCl濃度 / 木部液 / イネ / 根の水透過性 / 根の溶質透過性 / 生育段階 / 水温
Research Abstract

In order to develop a method for a quick measurement of the root capacity of water uptake and solute transport, we examined whether the root properties of water uptake and solute transport can be estimated by monitoring xylem pressure of a root (referred to the equipment as a root pressure probe) by using the theory of Steudle and Jescke based on the root function as an osmometer or not. The followings are obtained results in the research.
(1)Hydraulic conductivity measured under the steady state is considered to be reliable. Hydraulic conductivity measured with the root pressure probe under non-steady state was compared with that measured under the steady state in a maize root. There was a close linear correlation between the hydraulic conductivity obtained from both measurements.
(2)NaCl transport was investigated in the excised root of Rhizophora stylosa Griff. The reflection coefficient of the roots was smaller than 0.1 and was smaller remarkably than those previously reported for th … More e herbaceous species. The osmotic potential of the exudated sap from the excised root in the nutrient solutions containing various concentration of NaCl was always equivalent to that of the nutrient solution. These results indicate that NaCl transport accompanied by water is not restricted significantly in the excised root of Rhizophora stylosa Griff.
(3)The concentrations of Na^+ and Cl^-became equivalent to that of the nutrient solution in the root about 10 days after the seedlings of Rhizophora stylosa Griff, were transferred to the solution containing NaCl, but the concentration of a shoot remained low. This indicates that Na^+ and Cl^-transport from roots to shoot is restricted significantly. The calculated concentration of Na+ and Cl-in the transpirational stream in roots was also far low compared with the concentration of the nutrient solution. This indicates the transports of Na^+ and Cl^-into roots are also restricted.
(4)From the results mentioned above, we conclude that the method, considered in the research, can be employed in evaluating the root capacity of water uptake and, however, it is still required to be investigated whether this method can be adopted for evaluating root properties of solute transport. Less

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All 2005 2004 2003 2002 2001 Other

All Journal Article (14 results) Publications (10 results)

  • [Journal Article] Hydraulic conductivity and aquaporins of cortical cells in gravitropically bending roots of Pisum sativum L.2005

    • Author(s)
      Miyamoto, N.
    • Journal Title

      Plant Production Science 8(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hydraulic conductivity and aquaporins of cortical cells in gravitropically bending roots of Pisum sativum L.2005

    • Author(s)
      Miyamoto, N., M.Katsuhara, T.Ookawa, K.Kasamo, T.Hirasawa
    • Journal Title

      Plant Production Science 8(in press)

    • NAID

      110002961789

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Water uptake and hydraulic properties of elongating cells in hydrotropically bending roots of Pisum sativum L.2004

    • Author(s)
      Miyamoto, N., T.Ookawa, T.Takahashi, T.Hirasawa
    • Journal Title

      Plant Cell Physiology Vol.43(4)

      Pages: 393-401

    • NAID

      10008455354

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Growth responses of seminal roots of wheat seedlings to a reduction in the water potential of vermiculite.2004

    • Author(s)
      Akmal, M.
    • Journal Title

      Plant and Soil 267

      Pages: 319-328

    • Related Report
      2004 Annual Research Report
  • [Journal Article] バーミキュライトのNaCl濃度に対するオオムギ幼植物の成長反応の品種間差2004

    • Author(s)
      平沢 正
    • Journal Title

      日本作物学会関東支部会報 19

      Pages: 14-15

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 水耕液のNaCl濃度の急激な増加がマングローブ植物ヤエヤマヒルギの葉の水分状態およびNaCl含量に及ぼす影響2003

    • Author(s)
      丸野祐介
    • Journal Title

      日本作物学会紀事 72(別1)

      Pages: 246-247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of sudden increases of NaCl concentration of culture solution on water status and NaCl concentration in leaves of the mangrove Rhizophora stylosa2003

    • Author(s)
      Maruno, Y., T.Ookawa, K.Ishihara, T.Hirasawa
    • Journal Title

      Japan Journal of Crop Science 72(Extra 1)

      Pages: 246-247

    • NAID

      110001726044

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Water uptake and hydraulic properties of elongating cells in hydrotropically bending roots of Pisum sativum L.2002

    • Author(s)
      Miyamoto, N.
    • Journal Title

      Plant & Cell Physiology 43

      Pages: 393-401

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hydraulic conductivity of rice roots.2001

    • Author(s)
      Miyamoto, N.
    • Journal Title

      Journal of Experimental Botany 53

      Pages: 1835-1846

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 植物の体内水分調節と水輸送2001

    • Author(s)
      平沢 正
    • Journal Title

      日本作物学会紀事 70

      Pages: 477-488

    • NAID

      110001742413

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Properties of excised roots of the mangrove Rhizophora stylosa Griff. in NaCl transport.2001

    • Author(s)
      Maruno, Y.
    • Journal Title

      Proceedings of the 6^<th> Symposium of the International Society of Root Research

      Pages: 268-269

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hydraulic conductivity of rice roots2001

    • Author(s)
      Miyamoto, N., E.Steudle, T.Hirasawa, R.Lafitte
    • Journal Title

      Journal of Experomental Botany Vol.52

      Pages: 1835-1846

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reguration of water status and water transport in plants2001

    • Author(s)
      Hirasawa, T.
    • Journal Title

      Japanese Journal of Crop Science 70(4)

      Pages: 477-488

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Properties of excised roots of the mangrove Rhizophora stylosa Griff, in NaCl transport2001

    • Author(s)
      Maruno, Y., N.Miyamoto, Y.Mitsuhashi, T.Ookawa, T.Hirasawa
    • Journal Title

      Proceedings of the 6th Symposium of the International Society of Root Research

      Pages: 268-269

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 丸野佑介: "水耕液のNaCl濃度の急激な増加がマングローブ植物ヤエヤマヒルギの葉の水分状態およびNaCl含量に及ぼす影響"日本作物学会紀事. 72(別1). 246-247 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平沢 正: "蒸散速度、根の表面積が水稲の水の通導抵抗に及ぼす影響-「葉ざし」水稲を用いて"日本作物学会関東支部会報. 18. 48-49 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 平沢 正: "栽植様式の異なる湛水直播水稲の水の通導抵抗と光合成速度の日中低下の比較"日本作物学会関東支部会報. 18. 78-79 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyamoto, N: "Water uptake and hydraulic properties of elongating cells in hydrotropically bending roots of Pisum sativum L."Plant Cell Physiology. 43(4). 393-401 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 丸野佑介: "水耕液のNaCl濃度の急激な増加がマングローブ植物ヤエヤマヒルギの葉の水分状態およびNaCl含量に及ぼす影響"日本作物学会紀事. 73(別1)(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyamoto, N.: "Hydraulic conductivity of rice roots"Journal of Experimental Botany. 52. (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Maruno, Y.: "Properties of excised roots of the mangrove Rhizophora stylosa Griff. in NaCl transport"Proceedings of the 6th Symposium of the International Society of Root Research. 268-269 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyamoto, N.: "Cell hydraulic properties in hydrotropically bending roots of pea (Pisum sativum L.)"Proceedings of the 6th Symposium of the International Society of Root Research. 40-41 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyamoto, N.: "Hydraulic conductivity of adventitious roots of rice(Oryza sativa L.)"Japanese Journal of Crop Science. 70(Extraissue 1). 198-199 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyamoto, N.: "Water uptake and hydraulic properties of elongating cells in hydrotropically bending roots of Pisum sativum L."Plant & Cell Physiology. 43(In press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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