| Project/Area Number |
13556029
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General fisheries
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
FUKUYO Yasuwo Asian Natural Environmental Science Center, Associate Professor, アジア生物資源環境研究センター, 助教授 (10165318)
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Masaaki Kitasato University, School of Fisheries Sciences, Professor, 水産学部, 教授 (40050588)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | Light-emitting diode / Microalgae / Culture / Incubator / Dinoflagellate / Diatom / Toxic microalgae / Peltiert module / 微熱藻類 / 小型培養装置 / 携行培養装置 |
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| Research Abstract |
It is fundamental to establish clonal cultures from various part of world for study on biological, ecophysiological and biochemical features of harmful marine microalgae which are thought to have spread globally. But the culture establishment is sometimes difficult, because areas affected by the harmful microalgae are often under-developed coastal area and far from sophisticated laboratories equipped with culturing facilities.
This study aimed to develop a Portable incubation chamber furnishing temperature and light control devices. The light resource is obtained by Light Emitting Diodes (LEDs) and it poses many advantages on its smaller size, safety and low electric consumption. Various dinoflagellates and diatoms shows smooth growth comparable to natural light under red, green and blue lights from the LEDs. A dmoflagellate Heterocapsa cocularisquama grows well under red light, and Alexandrium spp under blue light. Different species shows small variation on optimum light condition (strength and color) for the highest growth rate, and it may come from difference of adaptability of species to lights from LEDs. Optimal spectra output in the region of photosynthetic pigment absorption should be found to use the very narrow spectrum output of LEDs. While the temperature could be regulated at a desired temperature between 10 and 30 degree in Celsius using a Peltiert module with a thermo controller, necessary electrical power is high and continuous use of the regulator may cause problem on the capacity of the chamber and maintenance cost of a battery. From these result a chamber (30x20x20 cm) was successfully made and used with good results for transfer of microalgae from the Philippines and Thailand to Japan. This portable incubation chamber enables the maintenance of living cells during handling and field work so that more cultures of harmful microalgae can be established from much wider location.
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