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Development of anti-obesity vaccine for domestic animals by regulation of gene expression during adipogensis. -Low fat meat production-

Research Project

Project/Area Number 13556043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied animal science
Research InstitutionTohoku University

Principal Investigator

AKIBA Yukio  Tohoku University, Graduate School of Agricultural Science, Professor, 大学院・農学研究科, 教授 (30005631)

Co-Investigator(Kenkyū-buntansha) HORIKAWA Hiroshi  Ito-chu feedstuff Co., Chief in research Center, 総合技術研究部, 研究所長
TAKAHASHI Kazuaki  Tohoku University, Graduate School of Agricultural Science, Assistant Professor, 大学院・農学研究科, 助手 (80183440)
SATO Kan  Tohoku University, Graduate School of Agricultural Science, Assistant Professor, 大学院・農学研究科, 助手 (20250730)
堀川 博  伊藤忠飼料株式会社, 総合技術研究部, 研究所長
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsfat deposition / adipocyte / chicken / regulatory factor of differentiation / differentiation marker / anti-obesity vaccine / transcriptional factor
Research Abstract

To develop an anti-obesity vaccine for domestic animals and a safety low fat meat production, we investigated 1)the regulatory mechanism of adipocyte differentiation on animal preadipocyte, 2)screening of functional nutrient for the inhibition of adipocyte hyperplasa, and 3)the effect of intraperitoneal administration with inhibitor of adipocyte proliferation on adipose tissue weight.
1)The regulatory mechanism of chicken adipocyte differentiation
To induce the chicken pre-adipocyte differentiation, we firstly clarified that the addition of an exogenous fatty acid mixed with hormones (Ins, DEX, IMX), which induce the differentiation of mammalian adipoocyte, was essential for chicken adipogensis. In addition, it is determine that PPARγ is master regulator of chicken adipocyte differentiation during adipogensis.
2) Screening of functional nutrient for the inhibition of adipocyte hyperplasa
The adipocyte proliferation declined with the addition of the mixture of insulin. 3-isobutyl-1-methylxanthin, dexamethason and oleic acid, and completely inhibition of cell growth occurred at the concentration of 20μg/ml insulin, 0.5mM 3-isobutyl-1-methylxanthin, 500nM dexamethason and 300μM oleic acid in the media.
3)The effect of intraperitoneal administration with inhibitor of adipocyte proliferation on adipose tissue weight
In chickens with single intraperitoneal injection of these hormone and fatty acid mixture made emulsion with triolein, adipose fat pad weight was significantly lower than the control chickens whereas the body weight decreased to a small extent. In addition, the administration of our anti-obesity vaccine regulated in adipocyte hyperplsia.
Unfortunately, it was not still known the powerful nutrient, which was easily accepted by consumer, for the regulation of adipocyte hyperplasia, but the new manipulation technique reducing fat deposition, called anti-obesity vaccine, was developed by the control of adipogensis in chickens.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Sato, K., Ohuchi A., Seol H.S., Toyomizu, M., Akiba Y.: "Changes in mRNA expression of 3-hydroxy-3-methylglutary coenzyme A reductase and cholesterol 7 alpha-hydroxylase in chickens."Biochim Biophys Acta. 1630. 96-102 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba T., Sato K., Tachibana S., Takahashi K., Nishida A., Akiba Y.: "Preparation of monoclonal antibody against chicken apolipoprotein B and development of enzyme liked immunosolvent assay(ELISA) method with the antibody aiming at the optimization of lipid metabolism in chickens."J.Poultry Sci.. 40. 212-220 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 佐藤幹, 秋葉征夫: "鶏脂質代謝の特性とその調節"畜産技術. 577. 2-6 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tachibana, S., Sato, K., Takahashi, T., Akiba, Y.: "Octanoate inhibits very low-density lipoprotein secretion in primary culture of chicken hepatocytes"Comp.Biochem.Physiol.A.. 132. 621-627 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato K., Takahashi, Y., Takahashi, T., Katoh, N., Akiba, Y.: "Identification of Factors Regulating Lipoprotein Lipase Catalyzed Hydrolysis in Rats with the Aid of Monoacid-Rich Lipoprotein Preparations."J.Nutri.Biochem.. 13. 528-538 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 佐藤幹, 秋葉征夫: "鶏における脂質代謝調節遺伝子の代謝的意義とその栄養修飾"栄養生理研究会報. 46. 121-136 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato K., Akiba Y.: "Lipoprotein lipase mRNA expressions in adipose tissues are little modified by aging and nutritional state in broiler chickens."Poultry Science. 81. 846-852 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, K., Ohuchi A., Seol H.S., Toyomizu, M., Akiba Y.: "Changes in mRNA expression of 3-hydroxy-3-methylglutary coenzyme A reductase and cholesterol 7 alpha-hydroxylase in chickens."Biochim Biophys Acta. 1630. 96-102 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chiba T., Sato K., Tachibana S., Takahashi K., Nishida A., Akiba Y.: "Preparation of monoclonal antibody against chicken apolipoprotein B and development of enzyme liked immunosolvent assay (ELISA) method with the antibody aiming at the optimization of lipid metabolism in Chickens."J.Poultry Sci.. 40. 212-220 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, K., Akiba, Y.: "Characterization and regulation of lipid metabolism in chickens."Livestock technology. 577. 2-6 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tachibana, S., Sato, K., Takahashi, T., Akiba, Y.: "Octanoate inhibits very low-density lipoprotein secretion in primary culture of chicken hepatocytes."Comp.Biochem.Physiol.A.Mol.Integr.Physiol.. 132. 621-627 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato K., Takahashi, Y., Takahashi, T., Katoh, N., Akiba, Y.: "Identification of Factors Regulating Lipoprotein Lipase Catalyzed Hydrolysis In Rats with the Aid of Monoacid-Rich Lipoprotein Preparations."J.Nutri.Biochem.. 13. 528-538 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, K., Akiba, Y.: "Lipoprotein lipase mRNA expressions in adipose tissues are little modified by aging and nutritional state in broiler chickens."Poultry Sci.. 81. 846-852 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, K., Akiba, Y.: "Identification and nutritional control of factors regulating chicken fat deposition."Journal of animal physiological and nutritional research. 46. 121-136 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato, K., Ohuchi A., Seol H.S., Toyomizu, M., Akiba Y.: "Changes in mRNA expression of 3-hydroxy-3-methylglutary coenzyme A reductase and cholesterol 7 alpha-hydroxylase chickens"Biochim Biophys Acta. 1630. 96-102 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Chiba T., Sato K., Tachibana S., Takahashi K., Nishida A., Akiba Y.: "Preparatin of monoclonal antibody against chicken apolipoprotein B and development of enzyme liked immunosolvent assay(ELISA)method with the antibody aiming at the optimization of lipid metabolism in chickens"J.Poultry Sci.. 40. 212-220 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤幹, 秋葉征夫: "鶏脂質代謝の特性とその調節"畜産技術. 577. 2-6 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tachibana, S., Sato, K., Takahashi, T., Akiba, Y.: "Octanoate inhibits very low density lipoprotein secretion in primary culture of chicken hepatocytes"Comp. Biochem. Physiol. A.. 132. 621-627 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato K., Akiba Y.: "Lipoprotein lipase mRNA expressions in adipose tissues are little modified by aging and nutritional state in broiler chickens"Poultry Science. 81. 846-852 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤幹, 秋葉征夫: "鶏における脂質代謝調節遺伝子の代謝的意義とその栄養修飾"栄養生理研究会報. 46. 121-136 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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