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Analysis for putative relationship between causative genes for hereditary disorder and quantitative traits loci in cattle

Research Project

Project/Area Number 13556044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied animal science
Research InstitutionHOKKAIDO UNIVERSITY (2002)
The University of Tokyo (2001)

Principal Investigator

INABA Mutsumi  Graduate School of Veterinary Medicine, Professor, 大学院・獣医学研究科, 教授 (00183179)

Co-Investigator(Kenkyū-buntansha) IMAGAWA Kazuhiko  Graduate School of Agriculture and Life Sciences, University of Tokyo, Associate Professor, 大学院・農学生命科学研究科, 助教授 (00291956)
OGAWA Hiroyuki  Graduate School of Agriculture and Life Sciences, University of Tokyo, Professor, 大学院・農学生命科学研究科, 教授 (30012016)
MAEDE Yoshimitsu  Graduate School of Veterinary Medicine, Professor, 大学院・獣医学研究科, 教授 (40002084)
INANAMI Osamu  Graduate School of Veterinary Medicine, Associate Professor, 大学院・獣医学研究科, 助教授 (10193559)
KOBAYASHI Masato  YamagataTechnopolis for Agriculture, Chief Scientist, 主任研究員
小野 憲一郎  東京大学, 大学院・農学生命科学研究科, 教授 (50111480)
印牧 美佐生  (社)家畜改良事業団, 家畜改良技術研究所, 遺伝子検査部長(研究職)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 2002: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥10,200,000 (Direct Cost: ¥10,200,000)
Keywordsgenetic disorders / beef quality / beef cattle / band 3 deficiency / claudin-16 deficiency / quantitative traits / red cells / kidney / 牛腎尿細管形成不全症
Research Abstract

A putative relationship between phenotypes for band 3 deficiency with the R664X mutation and contents of saturated/unsaturated fatty acids, which predominantly affect flavor and taste of beef, was evaluated. There was no direct linkage between phenotype/genotype and beef quality although carriers for band 3 deficiency appeared to have unsaturated fatty acids such as oleic acid at levels higher than normal animals. Moreover, we could not obtain direct evidence for remarkable advantage of homozygous/heterozygous states for claudin-16 deficiency. Present study, however, demonstrated a genetic factor that would regulate fatty acid contents located in chromosome 19, and suggested its linkage with fatnecrosis.
The findings on molecular pathobiology of band 3 deficiency indicated that the mutant band 3 (bebRX) plays a dominant-negative role on the expression of normal band 3 and a partner in the membrane skeleton, ankyrin, and the interaction of band 3 with ankyrin occurs on the ER membrane soon after band 3 synthesis is started during erythroid development. Total or partial deficiency of band 3 in erythroid and renal tubular cells appeared to cause acidosis, leading to downregulation of cell metabolism. Claudin-16 expressed in MDCK cells displayed overall reduction in paracellular transport of ions and solutes including calcium ion, while it appeared to form intercellular pathway for calcium, suggesting that heterophilic combinations of claudin-16 and other claudin proteins other than claudin-1/4 would be essential to formation of molecular pores for calcium.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Tamahara, S., ほか5名: "Nonessential roles of cysteine residues in functional expression and redox regulatory pathways for canine glutamate/aspartate transporter based on mutagenic analysis"Biochemical Journal. 367. 107-111 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsuki, N., ほか2名: "Catabolism of cytoplasmic and mitochondrial adenosine nucleotides in C2C12 skeletal myotube under chemical hypoxia"The Journal of Veterinary Medical Science. 64. 341-347 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato, K., ほか7名: "Cloning and characterization of excitatory amino acid transporters GLT-1 and EAAC1 in canine brain"The Journal of Veterinary medical Science. 63. 997-1002 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiraishi, M., ほか4名: "Platelet dysfunction in Chediak-Higashi syndrome-affected cattle"The Journal of Veterinary Medical Science. 64. 751-760 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohba, Y., ほか6名: "Renal reabsorption of magnesium and calcium by cattle with renal tubular dysplasia"The Veterinary Record.New Series. 151. 384-387 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, Y., ほか7名: "Pathological changes of renal tubular dysplasia in Japanese black cattle"The Veterinary Record.New Series. 150. 628-632 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamahara, S., Inaba, M., Sato, K., Matsuki, N., Hikasa, Y., Ono, K.: "Nonessential roles of cysteine residues in functional expression and redox regulatory pathways for canine glutamate/aspartate transporter based on mutagenic analysis"Biochem. J.. 367. 107-111 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsuki, M., Inaba, M., Ono, K.: "Catabolism of cytoplasmic and mitochondrial adenosine nucleotides in C2C12 skeletal myotube under chemical hypoxia"J. Vet. Med. Sci.. 64. 341-347 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato, K., Inaba, M., Baba, K., Tamahara, S., Koshino, I., Hikasa, Y., Ono, K., Kagota, K.: "Cloning and characterization of excitatory amino acid transporters GLT-1 and EAAC1 in canine brain"J. Vet. Med. Sci.. 63. 997-1002 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiraishi, M., Ogawa, H., Ikeda, M., Kawashima, S., and Ito, K.: "Platelet dysfunction in Chediak-Higashi syndrome-affected cattle"J. Vet. Med. Sci.. 64. 751-760 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohba, Y., Kitoh, K., Nakamura, H., Okura, H., Kunieda, T., Sasaki, Y., Kitagawa, H.: "Renal reabsorption of magnesium and calcium by cattle with renal dysplasia"Vet. Rec.. 151. 384-387 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, Y., Kitagawa, H., Kitoh, K., Okura, Y., Suzuki, M., Mizukoshi, M., Ohba, Y., Masegi, T.: "Pathological changes of renal tubular dysplasia in Japanese black cattle"Vet. Rec.. 150. 628-632 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamahara, S., ほか5名: "Non-essential roles of cysteine residues in functional expression and redox regulatory pathways for canine glutamate/aspartate transporter based on mutagenic analysis"Biochemical Journal. 367. 107-111 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Bonkobara, M., ほか5名: "Effects of epidermal growth factor on placental amino acids uptake in pregnant rats"The Journal of Veterinary Medical Science. 64. 689-692 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Thongsong, B., ほか6名: "Effects of insulin-like growth factor-1 on maternal and fetal plasma amino acid levels in pregnant rats"The Journal of Veterinary Medical Science. 64. 859-861 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohba, Y., ほか6名: "Renal reabsorption of magnesium and calcium by cattle with renal tubular dysplasia"The Veterinary record. New series. 151. 384-387 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sasaki, Y., ほか7名: "Pathological changes of renal tubular dysplasia in Japanese black cattle"The Veterinary record. New series. 150. 628-632 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato, K, 8名: "Cloning and characterization of excitatory amino acid transporters GLT-1 and EAAC1 in canine brain"Journal of Veterinary Medical Science. 63. 997-1002 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuki, N 3名: "Catabolism of cytoplasmic and mitochondrial adenosine nucleotides in C_2C_<12> skeletal myotube under chemical hypoxia"Journal of Veterinary Medical Science. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Boffi, F 6名: "Effects of chemical ischemia on purine nucleotides, free radical generation, lipid peroxidation and intracellular calcium levels in C2C12 myotube derived from mouse myocytes"Journal of Veterinary Medical Science. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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