| Project/Area Number |
13556051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied veterinary science
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
MASUDA Kenichi THE UNIVERSITY OF TOKYO, Graduate School of Agricultural and Life Sciences, Assistant (40313077)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMOTO Hajime 東京大学, Graduate School of Agricultural and Life Sciences, Professor (60163804)
OHNO Koichi 東京大学, Graduate School of Agricultural and Life Sciences, Associate Professor (90294660)
SAKAGUCHI Masashiro 国立感染症研究所, National Institute of Infectious Diseases, Dept.Immunology, Chief Researcher (20170590)
HIRAHARA Kazuki 三共株式会社, Sankyo, Co.Ltd., Researcher
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2002: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2001: ¥7,100,000 (Direct Cost: ¥7,100,000)
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| Keywords | dogs / cats / food hypersensitivity / lymphocyte blastogenic responses / T cell epitopes / vaccine / oral allergy syndrome / 抗原特異的ヒスタミン放出 / ペプチド療法 / 感受性 / リンパ球刺激試験 / 食物抗原 / ヒスタミン放出 |
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| Research Abstract |
In this study, pathogenesis of food allergy in dogs and cats has been investigated with immunological techniques. We found that types I and IV hypersensitivity against food allergens were involved in the pathogenesis of food hypersensitivity in dogs and cats. In terms of cross-reactivity between food and environmental allergens, oral allergy syndrome was reported in a dog sensitive to Japanese cedar pollen as a consequence of cross-reactivity of IgE between the cedar pollen and tomato antigen. We also developed an objective marker to identify offending food allergens by lymphocyte blastogenic responses against food allergens, resulting in a major contribution to veterinary clinical medicine for the diagnosis of food hypersensitivity in dogs. On the other hand, anaphylaxis against canine vaccine was epidemiologically suggested to be associated with food hypersensitivity in dogs. In order to develop novel immunotherapies, dogs experimentally induced allergy showed reduction of bronchial hypersensitivity to allergens by oral immunotherapy using T cell epitopes. We also found that a sequence of CpG motife that effectively induced IFN-g production from peripheral blood mononuclear lymphocytes.
Thus, in this study, a major part of pathogenesis of food hypersensitivity in dogs and cats was clearly elucidated, contributing to veterinary clinical medicine. In addition, since the pathogenesis of food hypersensitivity in dogs and cats was very similar to that in humans, these animals would be useful as a nanimal model of spontaneous food allergy.
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