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STUDY ON MECHANISM AND TREATMENT OF JET LAG CAUSED BY DISSOCIATION OF TWO OSILLATORS.

Research Project

Project/Area Number 13557007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionKINKI UNIVERSITY

Principal Investigator

SHIGEYOSHI Yasufumi  KINKI UNIVERSITY SCHOOL OF MEDICINE, Professor, 医学部, 助教授 (20275192)

Co-Investigator(Kenkyū-buntansha) NAKAHAMA Ken-ichi  KINKI UNIVERSITY SCHOOL OF MEDICINE, Assistant, 医学部, 助手 (60281515)
NAGANO Mamoru  KINKI UNIVERSITY SCHOOL OF MEDICINE, Assistant, 医学部, 助手 (80155960)
FUJIOKA Atsuko  KINKI UNIVERSITY SCHOOL OF MEDICINE, Assistant Professor, 医学部, 助教授 (30077664)
SATO Shinsuke  KINKI UNIVERSITY SCHOOL OF MEDICINE, Assistant, 医学部, 助手 (40270574)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsINTERNAL CLOCK / CIRCADIAN RHYTHM / CLOCK GENES / Rev-erb / JET LAG / Per1 / cAMP / SUPRACHIASMATIC NUCLEUS / 慨日周期 / 位相変位
Research Abstract

A. Dichotomy of the circadian center is associated with jet lag syndrome
In both 10 hour delay and 6 hour advance of light-dark cycle, we found that there appeared the dissociation of the suprachiasmatic into two oscillators. The ventrolateral region of the SCN (VLSCN) shifted rapidly whereas the dorsomedial regions of the SCN (DMSCN) shifted very slowly, spent more than a week to regain synchronization of the VLSCN and VLSCN. The sluggish shift of the DMSCN corresponds to the suppressed locomotors activity, which suggests that jet lag is caused by the slow shift of the DMSCN after LD cycle shift.
B. Transgene of mammalian Per2 genes to Per2-null Drosophila mutants recovered the circadian rhythmicity.
We introduced transgene to Per2-null Drosophila of which activity is arrhythmic. In the Drosophila expressing mouse Per21 or Per22 gene, we found the recovery of locomotor rhythms. The finding suggests that Pa-21 and Per22 have similar roles of clock genes even in the mammalian species.
C. A Transcription Factor Response Element for Gene Eypressinn During Circadian Night
We profiled suprachiasmatic nuclei (SCN) and liver genome-wide expression patterns under light/dark (LD) cycles and constant darkness (DD). We extensively determined transcription start sites (TSS) of human orthologues for newly identified cycling genes and then Per2 formed bioinformatical searches for relationships between time-of-day specific expression and transcription factor response elements around TSS. We demonstrate the role of the Rev-ErbA/ROR response element in gene expression of nocturnally expressed genes in SCN and liver.
D. Phosphodiesterase type,4 (PDE4) specifically degradg cAMP
To enhance Per21 transcription by the inhibition of cAMP degradation, we used Rolipram, a specific inhibitor of phosphodiesterase type 4 which degrades the cAMP. We found that the Per21 transcript increased transiently but the Per2iod length of Per21 increase was not extended.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Shigeyoshi Y, Meyer-Bernstein B, Yagita K, Fu W, Chen Y, Takumi T, Scbotland P, Sehgal A, Okamura H: "Restoration of circadian behavioural rhythms in a period null Drosophila mutant (per01) by mammalian period homologues mPer1 and mPer2"Genes Cells. 7(2). 163-171 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakahama K, Fujioka A, Nagano M, Satoh S, Furukawa K, Sasaki H, Shigeyoshi Y: "A role of the C-terminus of aquaporin 4 in its membrane expression in cultured astrocytes"Genes Cells. 7(7). 731-741 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueda HR, Chen W, Adachi A, Wakamatsu H, Hayashi Sm Takasugi T, Nagano M, Nakahama K, Suzuki Y, Sugano S, Iino M, Shigeyoshi Y, Hashimoto S: "A transcription factor response element for gene expression during circadian night"Nature. 418(68979). 534-539 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Ishida, K.Yagita, T.Fukuyama, M.Nishimura, M.Nagano, Y.Shigeyoshi, S.Yumaguchi, T Komori, H.Okamura: "Constitutive expression and delayed light response of casein kinase Iepsilon and Idelta mRNAs in the mouse Suprachiasmatic nucleus"Journal of Neuroscience Research. 64. 612-616 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Yammoto, Y.Shigeyoshi, Y.Ishida, T.Fukuyama, S.Yamaguchi, K.Yagita, T.Moriya, S.Shibata, N.Takashima, H.Okamura: "Expression of the Per1 gene in the hamster : brain atlas and circadian characteristics in the suprachiasmatic nucleus"Journal of Comparative Neurology. 430. 518-532 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Taguchi, R.Okura, K.Yagita, Y.Ishida, H.Onishi, Y.Shigeyoshi, S.Yamaguchi, M.Nishimura, S.Masubuchi, H.Okamura: "Different Circadian Expression Profiles of Clock Genes in the Mouse Suprachiasmatic Nucleus"Biomedical Research. 22(3). 125-131 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura, T., Shigeyoshi, Y., Maebayashi, Y., Yamaguchi, S., Yagita, K., Okamura, H.: "Different developmental profiles of the expression of preprosomatostatin and preprotachykinin-A mRNAs in rat SCN neurons"Dev.Brain Research. 127. 81-86 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueda, H. U., Chen, W., Adachi, A., Wakamatsu, H., Hayashi, S., Takasugi, T., Nagano, N., Nakahama, K., Suzuki, Y., Sugano, S., Iino, M., Shigeyoshi. Y. and Hashimoto, S.: "A Transcription Factor Response Element for Gene Expression During Circadian Night."Nature. 418. 534-539 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakahama, K., Fujioka, A., Nagano, M., Satoh, S., Furukawa, K., Sasaki, H., and Shigeyoshi. Y.: "A role of the C-terminus of aquaporin 4 in its membrane pxpression in cultured astrocytes"Genes Cells. 7. 731-741 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeyoshi. Y., Meyer-Bernstein, E., Yagita, K., Fu, W., Chen, Y., Takumi, T., Schotland, P., Sehgal, A., and Okamura, H: "Restoration of circadian behavioral rhythms in a period null Drosophila mutant (per01)by mammalian period homologues mPerl and mPer2."Genes Cells. 7. 163-171 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Taguchi, K., Okura, R., Yagita, K., Ishida, Y., Onishi, H., Shigeyoshi. Y., Yamaguchi. S., Nishimura, M., Masubuchi, S., and Okamura, H: "Different Circadian Expression Profiles of Clock Genes in the Mouse Suprachiasmatic Nuclues."Biomedical Journal. 22. 125-131 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishida Y, Yagita K, Fukuyama T, Nishimura M, Nagano M, Shigevoshi Y.Yamagucni S, Komori T, Okamura H.: "Constitutive expression and delayed light response of casein kinase Iepsilon and Idelta mRNAs in the mouse Suprachiasmatic nucleus."J Neurosti Res.. 15,64. 612-616 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto, S., Shigeyoshi. Y., Ishida, Y., Fukuyama, T., Yamaguchi, S., Yagita, K., Moriya, Y., Shibata, S., Yakashima, N., and Okamura, H: "Expression of the Per1 gene in the Hamster: Brain Atlas and Circadian Characteristics in the Suprachiasmatic Nucleus."J. Comp. Neurol.. 430. 518-532 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakaniura, T., Shigeyoshi. Y., Maebayashi, Y., Yamagucni, S., Yagita, K., and Okamura, H,: "Different developmental profiles of the expression of preprosomatostatin and preprotachykinin-A mRNAs in rat SCN neurons."Dev. Brain Research. 127. 81-86 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeyoshi Y, Meyer-Bernstein B, Yagita K, Fu W, Chen Y, Takumi T, Schotland P, Sehgal A, Okamura H: "Restoration of circadian behavioural rhythms in a period null Drosophila mutant (per01) by mammalian period homologues mPer1 and mPer2"Genes Cells. 7(2). 163-171 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakahama K, Fujioka A, Nagano M, Satoh S, Furukawa K, Sasaki H, Shigeyoshi Y: "A role of the C-termnius of aquaporin 4 in its membrane expression in cultured astrocytes"Genes Cells. 7(7). 731-741 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueda HR, Chen W, Adachi A, Wakamatsu H, Hayashi I, Takasugi T, Nagano M, Nakahama K, Suzuki Y, Suga S, Iino M, Shigeyoshi Y, Hashimoto S: "A transcription factor response element for gene expression during circadian night"Nature. 418(68979). 534-539 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Ishida, K.Yagita, T.Fukuyama, M.Nishimura, M.Nagano, Y.Shigeyoshi, S.Yamaguchi, T.Komori, H.Okamura: "Constitutive expression and delayed light response of casein kinase lepsion and Idelta mRNAs in the mouse suprachiasmatic nucleus"Journal of Neuroscience Research. 64. 612-616 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Yammoto, Y.Shigeyoshi, Y.Ishida, T.Fukuyama, S.Yamaguchi, K.Yagita, T.Moriya, S.Shibata, N.Takashima, H.Okamura: "Expression of the Per1 gene in the hamster : brain atlas and circadian characteristics in the suprachiasmatic nucleus"Journal of Comparative Neurology. 430. 518-532 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Taguchi, R.Okura, K.Yagita, Y.Ishida, H.Onishi, Y.Shigeyoshi, S.Yamaguchi, M.Nishimura, S.Masubuchi, H.Oka: "Different Circadian Expression Profiles of Clock Genes in the Mouse Suprachiasmatic Nucleus"Biomedical Research. 22(3). 125-131 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamura, T., Shigeyoshi, Y., Maebayashi, Y., Yamaguchi, S., Yagita, K., Okamura, H.: "Different developmental profiles of the expression of preprosomatostatin and preprotachykinin-A mRNAs in rat SCN neurons"Dev. Brain Research. 127. 81-86 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y Ishida, K Yagita, T Fukuyama, M Nishimura, M Nagano, Y Shigeyoshi, S Yamaguchi. T Komori, H Okamura: "Constitutive expression and delayed light response of casein kinase Iepsilon and Idelta mRNAs in the mouse suprachiasmatic nucleus"Journal of Neuroscience Research. 64. 612-616 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Yammoto, Y.Shigeyoshi, Y.Ishida, T.Fukuyama, S.Yamaguchi, K.Yagita, T.Moriya, S.Shibata, N.Takashima, H.Okamura: "Expression of the Per1 gene in the hamster : brain atlas and circadian characteristics in the suprachiasmatic nucleus"Journal of Comparative Neurology. 430. 518-532 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Taguchi, R.Okura, K.Yagita, Y.Ishida, H.Onishi, Y.Shigeyoshi, S.Yamaguchi, M.Nishimura, S.Masubuchi, H.Okamura: "Different Circadian Expression Profiles of Clock Genes in the Mouse Suprachiasmatic Nucleus"Biomedical Research. 22(3). 125-131 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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