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Molecular Analysis of homologous recombination-mediated gene trageting

Research Project

Project/Area Number 13557010
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General medical chemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TAKEDA Shunichi  Kyoto University, Faculty of Medicine, Professor, 医学研究科, 教授 (60188191)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Daisuke  Kirin Brewery Co., LTD. Basic Technology Institute Researcher, 基盤技術研究所, 研究員
YAMAZOE Mitsuyoshi  Kyoto University, Faculty of Medicine, Assistant Professor, 医学研究科, 助手 (00284745)
SONODA Eiichiro  Kyoto University, Faculty of Medicine, Associate Professor, 医学研究科, 助教授 (50281093)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2002: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2001: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsDNA damage / DNA replication / Translesion DNA synthesis / Homologous DNA recombination / DNA polymerase / DT40 / Sister chromatid exchange / 相同DM組換え / 相同DNA組み換え / DNA修復 / チェックポイント / DT40細胞 / 遺伝子治療
Research Abstract

During each cell cycle replication forks inevitably stall at damaged DNA. The ability to overcome DNA lesions is an essential feature of the replication machinery. A large variety of specialized polymerases have recently been discovered that enable cells to replicate past various forms of damage. Alternatively homologous recombination can be used to restart DNA replication across the lesion. Genetic studies have shed light on the impact of these two post replication repair pathways in bacteria and yeast. In vertebrates, however, a genetic approach to study post replication repair has been compromised because most of the genes involved appear to be essential for embryonic development. We have taken advantage of the chicken cell line DT40 to perform a genetic analysis of translesion synthesis and homologous recombination and to characterize genetic interactions between these two pathways in vertebrates. In this article we aim to summarize our current understanding of post replication repair in DT40 in the perspective of results from other model systems.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Okada, T., Sonoda, E., Yamashita, Y.M., Koyoshi, S., Tateishi, S., Yamaizumi, M., Takata, Y., Ogawa, O., Takeda, S.: "Involvement of Vertebrate Pol k in Rad18-independent Postreplication Repair of UV damage"J. Biol. Chem. 277. 48690-48695 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashita, Y.M., Okada, T., Matsusaka, T, Sonoda l, E., Cho, Z., Araki, K., Tateishi, S., Yamaizumi, M., Takeda, S.: "RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells"EMBO J. 21. 5558-5566 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.J.Cumbers, G.T.Williams, S.L.Davies, R.L.Grenfell, S.Takeda, F.D.Batista, J.E.Sale, M.S.Neuberger: "Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines"Nature Biotechnology. 20. 1129-1134 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tauchi H, Kobayashi J, Morishima K, van Gent DC, Shiraishi T, Verkaik NS, vanHeems D, Ito E, Nakamura A, Sonoda E, Takata M, Takeda S, Matsuura S, Komatsu K: "Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells"Nature. 420. 93-98 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takata, M., Tachiiri, S., Fujimori, A., Thompson, L.H., Miki.Y., Hiraoka, M., Takeda, S., Yamazoe, M.: "Conserved domains in the chicken homologue of BRCA2"Oncogene. 21. 1130-1134 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] J.Henry-Mowatt1, D.Jackson, J.-Y.Masson, P.A.Johnson, P.M.Clements, F.E.Benson, L.H.Thompson, S.Takeda, S.C.West: "XRCC3 and RAD51 Modulate Replication Fork Progression on Damaged Vertebrate Chromosomes"Mol. Cell. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okada, T., Sonoda, E., Yamashita, Y. M., Koyoshi, S., Tateishi, S., Yamaizumi, M., Takata, M., Ogawa, O., and Takeda, S.: "Involvement of Vertebrate Pol k in Rad18-independent Postreplication Repair of UV damage"J. Biol. Chem. 277. 48690-48695 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashita, Y. M., Okada, T., Matsusaka, T., Sonoda, E., Cho, Z., Araki, K., Tateishi, S., Yamaizumi, M., and Takeda, S.: "RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells"EMBO J. 21. 5558-5566 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. J. Cumbers, G. T. Williams, S. L. Davies, R. L. Grenfell, S. Takeda, F. D. Batista, J. E. Sale, M. S. Neuberger: "Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines"Nature Biotechnology. 20. 1129-1134 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tauchi H, Kobayashi J, Morishima K, van Gent DC, Shiraishi T, Verkaik NS, van Heems D, Ito E, Nakamura A, Sonoda E, Takata M. Takeda S, Matsuura S, Komatsu K: "Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells"Nature. 420. 93-98 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takata, M., Tachiiri, S., Fujimori, A., Thompson, L. H., Miki, Y., Hiraoka, M., Takeda, S., and Yamazoe, M.: "Conserved domains in the chicken homologue of BRCA2"Oncogene. 21. 1130-1134 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] J. Henry-Mowattl, D. Jackson, J. -Y. Masson, P. A. Johnson, P. M. Clements, F. E. Benson, L. H, Thompson, S. Takeda, S. C. West, and K. W. Caldecott: Mol. Cell. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okada, T., Sonoda, E., Yamashita, Y.M., Koyoshi, S., Tateishi, S., Yamaizumi.M., Takata, M., Ogawa, O., Takeda, S.: "Involvement of Vertebrate Pol k in Rad18-independent Postreplication Repair of UV damage"J.Biol.Chem. 277. 48690-48695 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamashita, Y.M., Okada, T., Matsusaka, T., Sonodal, E., Cho, Z., Araki, K., Tateishi, S., Yarnaizumi, M., Takeda, S.: "RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells"EMBO J. 21. 5558-5566 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.J.Cumbers, G.T.Williams, S.L.Davies, R.L.Grenfell, S.Takeda, F.D.Batista, J.E.Sale, M.S.Neuberger: "Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines"Nature Biotechnology. 20. 1129-1134 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tauhi H, Kobayashi J, Morishima K, van Gent DC, Shiraishi T, Verkaik NS, vanHeems D, Ito E, Nakamura A, Sonoda E, Takata M, Tkakeda S, Matsuura S, Komatsu K: "Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells"Nature. 420. 93-98 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takata, M., Tachiiri, S., Fujimori, A., Thompson, L.H., Miki, Y., Hiraoka, M., Takeda, S., Yamazoe, M.: "Conserved domains in the chicken homologue of BRCA2"Oncogene. 21. 1130-1134 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] J.Henry-Mowattl, D.Jackson, J.-Y.Masson, P.A.Johnson, P.M.Clements, F.E.Benson, L.H.Thompson, S.Takeda, S.C.West: "XRCC3 and RAD51 Modulate Replication Fork Progression on Damaged Vertebrate Chromosomes"Mol.Cell. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Takata: "Chromosome instability and defective recombinational repair in knockout mutants of the five Rad51 paralogs"Mol.Cell.Biol.. 21. 2858-2866 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Fujimori: "Rad52 partially substitutes for the Rad51 paralog XRCC3 in maintaining chromosomal integrity in vertebrate cells"EMBO J. 20. 5513-5520 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sale, J.E.: "Ablation of XRCC2/3 transforms immunoglobulin V gene conversion into somatic hypermutation"Nature. 412:9. (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Fukushima: "Genetic analysis of the DNA-dependent protein kinase reveals an inhibitory role of Ku in late S-G2 phase DNA double-strand break repair"J. of Biological Chemistry. Vol.276. 44413-44418 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Takata: "Conserved domains in the chicken homologue of BRCA2"Oncogene. (in press). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] E.Sonoda: "Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells"Developmental Cell. Vol.1. 759-770 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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