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Assay Development of urinary 3-hydroxproline derived from basement membrane collagen for cancer screening

Research Project

Project/Area Number 13557034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Public health/Health science
Research InstitutionTokai University

Principal Investigator

OKAZAKI Isao  Tokai University, School of Medicine, Professor, 医学部, 教授 (00051649)

Co-Investigator(Kenkyū-buntansha) WATANABE Tetsu  Tokai University, School of Medicine, Associate Professor, 医学部, 助教授 (10129744)
HAYASHI Toshihiko  Tokyo University Graduate School, Faculty of Science and Arts, Professor, 大学院・総合文化研究科, 教授 (60090528)
MATSUYAMA Shigeo  Defense Medical College, Institute of Research Assistance, Assistant Professor, 共同利用研究施設, 講師
FURUYA Hiroyuki  Tokai University, School of Medicine, Assistant Professor, 医学部, 講師 (10276793)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥4,700,000 (Direct Cost: ¥4,700,000)
Keywordscancer screening / 3 - hydroxyproline / urinary excretion / type IV collagen / community health / 地域保健
Research Abstract

The Welfare and Health Law for The Elderly in Japan has requested the cancer screening for stomach, lung, breast, uterus and colon. The budget to support the law, however, is not included in the Japan Government, neither in the Prefectural Government. The local governments for citizens are wondering about cancer screening service by public support. We propose all purpose cancer screening system which means the target organ is not specialized. Okazaki et al. published an assay method as ELISA kit for measuring urinary 3-Hydroxyproline. The first year (2001) we succeeded in synthesis of Cys (3Hyp - 4Hyp - Gly)3 and Cys (Prol - 4Hyp - Gly)3 as antigen to get the antibody in rabbits. The latter peptide was also administered to rule out the specific antibody against 3Hyp - containing peptide. We succeeded again to establish a new ELISA kit for urinary 3 - hyp - containing peptide. We applied this new method for 199 healthy persons and 10 cancer patients. The values of urinary 3Hyp content measured by conventional method and ELISA kit method were parallel. The big difference was seen between the value in healthy persons and that in cancer patients.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Watanabe T, et al.: "Dynamic change of cells expressing MMP-2 and MT1-MMP mRNA in the recovery from liver fibrosis in the rat"Journal of Hepatology. 35. 465-473 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 松山重雄, 丸田明枝, ほか: "尿3-Hydroxyprolineによるがん検診への応用"Connective Tissue. 33(2). 135-135 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 古屋博行, 伊藤 俊, 岡崎 勲: "肺がん"医学と疫学. 48(2). 171-177 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Watanabe T, et al.: "Neural stem/progenitor cells appear in the recovery from liver fibrosis"Hepatology. 36(4). 248-248 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inagaki Y, et al.: "Interferon α antagonizes transforming growth factor β-stimulated collagen gene transcription and suppresses promoter activation"Hepatology. 36(4). 247-247 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 岡崎 勲, 新岡 真希: "消化器ナビゲーター(滝川 一編)"細胞外マトリックス. 2 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsuyama S, Maruta A, Okazaki I, et al.: "An application of urinary 3-hydroxyproline to cancer Screening"Connective Tissue. 32(2). 135 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Watanabe T, Niioka M, Ishikawa A, et al.: "Dynamic change of cells expressing MMP-2 and MT1-MMP mRNA in the recovery from liver fibrosis in the rat"Journal of Hepatology. 35. 465-473 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Furuya H. and Okazaki I.: "Lung Cancer"Medicine and Pharmacology. 48. 171-177 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okazaki I, Ninomiya Y, Friedman SL, Tanikawa K (editors), (ed. by Tanikawa H): "Extracellular Matrix and the Liver-approach to gene Therapy"Gastroenterology Navigator, Medical Review Pub Co., Tokyo. 32-33 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inagaki Y, Nemoto T, Watanabe T, Okazaki I: "Interferon a antagonizes transforming growth factor b-Stimulated collagen gene transcription and suppresses promoter activation"The 53rd Annual Meeting of The American Association for The Study of Liver Diseases, Boston,(Hepatology, 36 (4pt 2), 274A, 2002). 1-5 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Watanabe T, Niioka M, Sugioka Y, Inagaki Y, et al.: "Neural stem/progenitor cells appear in the recovery from liver fibrosis in the rat"The 53rd Annual Meeting of The American Association for The Study of Liver Diseases, Boston,(Hepatology, 36 (4pt 2), 248A, 2002). 1-5 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 古屋博行, 伊藤 俊, 岡崎 勳: "肺がん"医学と疫学. 48(2). 171-177 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Watanaba T, et al.: "Neural stem/progenitor cells appear in the recovery from liver fibrosis"Hepatology. 36(4). 248-248 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inagaki Y, et al.: "Interferon α antagonizes transforming growth factor β-stimulated collagen gene transcription and suppresses promoter activation"Hepatology. 36(4). 247-247 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 岡崎 勳, 新岡 真希: "消化器ナビゲーター (滝川 一 編)"細胞外マトリックス. 2 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松山重雄, 丸田明枝ほか: "尿3-hydroxyprolineによるがん検診への応用"Connective Tissue. 33(2). 135 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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