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Investigation for matching bHLH overexpressed transgenic mice to mood disorders model mice

Research Project

Project/Area Number 13557079
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Psychiatric science
Research InstitutionSt Marianna University School of Medicine

Principal Investigator

OSAKA Kenichi  St. Marianna University School of Medicine, Department Neuropsychiatry, Associated Professor, 医学部, 講師 (20233504)

Co-Investigator(Kenkyū-buntansha) MATUI Kiroaki  St. Mariaanna University School of Medicine, Radioisotope Research Institute, Professor, 医学部, 教授 (90181685)
ASAKURA Mikio  St.Marianna University, School of Medicine, Department of Neuropsychiatry, Assistant Professor, 医学部, 助教授 (70103504)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2003: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2001: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsbHLH / Mod stabilizer / Lithium / GAP-43 / MATH / Transcription Factor / eEF2 / E-box / ゲルシフトアッセイ / BHLH
Research Abstract

Lithium is a mood stabilizer used in treatment of bipolar disorders. We reported that the GAP-43 RNA expression and GAP-43 protein levels 1.5 times increased after 4-5 weeks lithium treatment in the rat brain. GAP-43 is known to play an important role in the releasing process for several neurotransmitters (Ach, 5-HT and DA). We also reported that MATH-2 transcription factor bind the GAP-43 promoter ad increased in the expression for GAP-43.
MATH-2 increased the activity about 2.5 times compared with control in luciferase assay, and MATH-2 regulated GAP-43 gene expression than the other transcription factors. 5 week lithium treatment increased in the MATH-2 protein level about 4 times by immunoblot. Long-term lithium treatment translocated MATH-2 to nucleolus and nuclear membrane by immunohistochemistry.
We considered that MATH-2 induced increase of GAP-43 protein level and the long-term lithium treatment regulated GAP-43 by MATH-2.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report

Research Products

(38 results)

All Other

All Publications (38 results)

  • [Publications] 長田賢一: "気分安定薬により変化するbHLH転写調節因子の検討"精神薬療研究年報. 34. 52-57 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長谷川 洋: "ラット大脳におけるリチウム長期投与中のE-BOX結合蛋白によるGAP-43mRNAの増加"日本神経精神薬理学雑誌. 21. 327 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "Chronic carabamazepine down regulated calcineurin and increased phosphorylation of GAP-43 amd MARCKS."The Japanese Journal of Pharmacology. 85. 262 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 御園生篤志: "リチウムと抗うつ薬併用投与後のラット大脳皮質におけるGAP-43のリン酸化の変化"日本神経精神薬理学雑誌. 21. 325 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長田 賢一: "気分安定薬の分子薬理学的作用機序からみた双極性感情障害の病態メカニズム"分子精神医学. 1. 16-24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長田 賢一: "中枢神経系における遺伝子発現異常"聖マリアンナ医科大学雑誌. 29. 611-618 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長田 賢一: "リチウムにより変化するMATH-2転写因子の検討"日本神経精神薬理学雑誌. 23. 262 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長谷川 洋: "カルバマゼピン使用中における免疫機構変化について"脳と精神の医学. 13. 201-209 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長田 賢一: "リチウムによるE-box結合蛋白の変化"生化学. 74. 1005 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長田 賢一: "神経細胞死から神経再生への分子メカニズム"精神科. 1. 489-493 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hasegawa, H.: "Chronic carbamazepine treatment increases myristoylated alanine-rich C kinase substrate phosphorylation in the rat cerebral cortex via down-regulation of calcineurin Aα."Brain research. 994. 19-26 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長田 賢一: "気分安定薬により変化するbHLH転写調節因子(MATH)の検討"精神薬療研究年報. 35. 209-216 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長田 賢一: "リチウム長期投与後変化する転写因子の解析"精神薬療研究年報. 26. 180-187 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "The investigation of the bHLH transcription factor changed by mood stabilizer"Annual Report of Pharmacopsychiatry Research. 34. 52-57 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Hasegawa: "Increased in GAP-43RNA induced by E-box binding protein after long-term treatment with lithium"Jpn.J.Neuropsychopharmacol. 21. 327 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "Chronic carabamazepine down regulated calcineurin and increased phosphorylation of GAP-43 and MARCKS"The Japanese Journal of Pharmacology. 85. 262 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Misonoo: "The change of GAP-43 phosphorylation after lithium treatment combined with antidepressants in rat brain"Jpn.J.Neuropsychopharmacol. 21. 325 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "The disfunction of gene expression in the central nerve system"The St.Marianna J.. 29. 611-618 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "The molecular mechanisms of biopolar disorders by the pharmacological effect of mood stabulizers"Jpn.J.Molecular Psychiatry. 1(2). 125-132 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Hasegawa: "Immunological changes during carbamazepine therapy in psychiatric patients"Brain Science and Mental Disorders. 13(2). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "The influence of E-box binding protein by lithium"Biochemistry. 74. 1005 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "The molecular mechanisms from apotosis to neuronal regeneration"Psychiatry. 1. 489-493 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Hasegawa: "Chronic carbamazepine treatment increases myristoylated alanine-rich C kinase substrate phosphorylation in the rat cerebral cortex via down-regulation of calcineurin Aa"Brain research. 994. 19-26 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "The study of the bHLH transcription factor (MATH) changed by mood stabilizer"Annual Report of Pharmacopsychiatry Research. 35. 209-216 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "The investigation of MATH-2 transcription factor after long-term lihitum treatment on rat brain"Jpn.J.Neuropsychopharmacol. 23. 262 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Osada: "The analysis of MATH-2 transcription factor after long-term treatment with lihitum"Annual Report f Pharmacopsychiatry Research. 36. 180-187 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 長田 賢一: "リチウム長期投与後変化する転写因子の解析"精神薬療研究年報. 36. 1-8 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Hasegawa: "Chronic carbamazepine treatment increases myristoylated alanine-rich C kinase substrate phosphorylation in the rat cerebral cortex via down-regulation of calcineurin Aα"Brain research. 994. 19-26 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 長田 賢一: "リチウムにより変化するMATH-2転写因子の検討"日本神経精神薬理学雑誌. 23. 262 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 田中大輔: "慢性変動ストレス後のラット青斑核のチロシン水酸化酵素とCRHニューロンシステムの関連性"日本神経精神薬理学雑誌. 23. 255 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 長田 賢一, 御園生 篤志, 長谷川 洋, 金井 重人, 朝倉 幹雄, 他: "気分安定薬により変化するbHLH転写調節因子(MATH)の検討"精神薬療研究年報. 35. (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 長田賢一, 長谷川 洋, 御園生篤志, 藤井佐知子, 長島秀明, 朝倉幹雄: "気分安定薬の分子薬理学的作用機序からみた双極性感情障害の病態メカニズム"分子精神医学. 1. 16-24 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 長田 賢一: "中枢神経系における遺伝子発現異常"聖マリアンナ医科大学雑誌. 29. 611-618 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 長田賢一, 御園生篤志, 長谷川洋, 田中大輔, 長島秀明, 朝倉幹雄 他: "リチウムによるE-box結合蛋白の変化"生化学. 74. 1005 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 長谷川 洋, 長田 賢一, 朝倉 幹雄: "カルバマゼピン使用中における免疫機構変化について"脳と精神の医学. 13. 201-209 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 長田賢一, 長谷川 洋, 御園生篤志, 田中大輔, 長島秀明, 朝倉幹雄 他: "神経細胞死から神経再生への分子メカニズム"精神科. 1. 489-493 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 長田 賢一: "気分安定薬により変化するbHLH転写調節因子の検討"精神薬療研究年報. 34. (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 長谷川 浩: "ラット大脳におけるリチウム長期投与中のE-BOX結合蛋白によるGAP-43mRNAの増加"日本神経精神薬理学雑誌. 21. 327 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-03-31   Modified: 2016-04-21  

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