| Project/Area Number |
13557087
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Endocrinology
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
IIRI Taroh The University of Tokyo, Faculty of Medicine, Researoh Associate, 医学部附属病院, 助手 (90313022)
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| Co-Investigator(Kenkyū-buntansha) |
OHNISHI Hirohide The University of Tokyo, Faculty of Medicine, Researoh Associate, 医学部附属病院, 助手 (00313023)
FUJITA Toshiro The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (10114125)
安田 宏 東京大学, 医学部・附属病院, 助手 (80262129)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 2002: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2001: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | G PROTEIN / G PROTEIN-COUPLED RECEPTOR / MOLECULAR MECHANISM / G PROTEIN DISEASE |
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| Research Abstract |
(1) Development of Method to Analyze Receptor-G Protein Interaction : By using DEAE-adenov irus method, we developed an easy method to analyze receptor dependent activation of G protein.
(2) Specificity and Mechanism of Receptor-G Protein Interaction : We analyzed (a) angiotensin II receptors (AT1 and AT2), (b) adrenergic receptors (β1-AR and β2-AR), and (c) mechanism of receptor-dependent activation of G protein.
(3) Analysis of Drugs Acting on G Protein-Coupled Receptors : We analyzed activities of drugs working as agonists, antagonists, inverse agonists on G protein-coupled receptors.
(4) Strategy to Make and Inprove Dominant Negative G proteins : We previously designed dominant negative Gsα (PNAS 96 : 499-504, 1999). By introducing newly discovered mutant Gα, we improved its activity.
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