| Project/Area Number |
13557088
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Endocrinology
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| Research Institution | Fukui Medical University |
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Principal Investigator |
MIYAMOTO Kaoru Fukui Medical University, Faculty of Medicine, Professor, 医学部, 教授 (30125877)
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| Co-Investigator(Kenkyū-buntansha) |
MINEGISHI Takashi Gunma University School of Medicine, Professor, 医学部, 教授 (00209842)
MIZUTANI Tetsuya Fukui Medical University, Faculty of Medicine, Research Associate, 医学部, 助手 (90322734)
YAMADA Kazuya Fukui Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20263238)
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| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 2002: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2001: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | Microarray / Subtraction cloning / Dioxin / Endocrine disrupters |
|---|
| Research Abstract |
In this study, we tried to develop a new system which can detect very minute amount of endocrine disrupters in our environment based on a microarray technology. By subtracting cDNAs from dioxin treated rat ovarian granulosa cells from those from control cells, we developed a subtraction cDNA library where dioxin inducible genes are concentrated. We then identified dioxin inducible genes by nucleotide sequence analysis of all clones. By that way, we could develop a cDNA microarray on which only dioxin inducible genes are spotted. By the present study, a new sensitive method for detecting very low concentrations of dioxin has been developed.
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