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Research for clinical application of electro-gene therapy against malignant brain tumor

Research Project

Project/Area Number 13557119
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Cerebral neurosurgery
Research InstitutionKumamoto University

Principal Investigator

KOCHI Masato  Kumamoto University, School of Medicine, Department of Neurosurgery, Associate Professor, 医学部, 助教授 (70178218)

Co-Investigator(Kenkyū-buntansha) SAKATA Tsuneaki  Shionogi Pharmacological corporation, Chief investigator, 医科学研究所, 主任研究員
USHIO Yukitaka  Kumamoto University, School of Medicine, Department of Neurosurgery, Professor, 医学部, 教授 (20028583)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordsmalignant glioma / electro-gene therapy / brain tumor / MCP-1 / IL-12 / suicide gene / clinical application / monkey / 脳腫瘍モデル / 安全性 / グリオーマ
Research Abstract

The prognosis of malignant glioma patients has not been improved in these 10 years, therefore, many investigators have become to consider that radiotherapy and chemotherapy would not be able to cure the malignant glioma. We have developed electro-gene therapy which can effectively and safely induce the DNA into the targeted cells within the human body. The purpose of this project was to make the electro-gene therapy close to the clinical application. Tne first step of this project was to examine the optimum conditions such as the voltage, pulse and the shape of the electrode and we established the several conditions. Tne next step was to examine the effectiveness of this method using the therapeutical DNAs and animal models of subcutaneous tumor or twain tumor. We could select several genes such as MCP-1, IL-12 or several suicide genes to reduce the tumor in the animal models. We also histopathologically and based on the behavioral science examined the safety and the side effect of this therapy by using the animal models. Based on our results we concluded that this electro-gene therapy might be effective for the malignant and invasive brain tumor and we also had the possibility to realize the clinical application of this therapy. However it is necessary to try this method in the animals evolutionally close to human such as monkeys.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kimura: "Expression of lymphocyte-specific chemokines in human malignant glioma : Essential role of LARC in cellular immunity of malignant glioma"International Journal of Oncology. 21(4). 707-715 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiraishi S: "Influence of p53 mutations on prognosis of patients with glioblastoma"Cancer. 95(2). 249-257 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kamiryo T: "Analysis of homozygous deletion of the p16 gane and correlation with survival in patients with glioblastoma multiforme"Journal of Neurosurgery. 96(5). 815-822 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nitta M: "Hyperploidy induced by drugs that inhibit formation of microtubule promotes chromosome instability"Genes to Cell. 7(2). 151-162 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tada K: "Analysis of loss of heterozygosity on chromosome 10 in patients with malignant astrocytic tumors : correlation with patient age and survival"Journal of Neurosurgery. 95(4). 651-659 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura T: "Intratumoral delivery of interleukin 12 expression plasmids with in vivo electroporation is effective for colon and renal cancer"Human Gene Therapy. 12(10). 1265-1276 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kimura et al.: "Expression of lymphocyte-specific chemokines in human malignant glioma Essential role of LARC in cellular immunity of malignant glioma."International Journal of Oncology. 21(4). 707-715 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiraishi S et al.: "Influence of p53 mutations on prognosis of patients with glioblastoma."Cancer. 95(2). 249-257 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kamijyo T et al.: "Analysis of homozygous deletion of the p16 gene and correlation wiih survival in patients with glioblastoma multiforme."Journal of Neurosurgery. 96(5). 815-822 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nitta M et al.: "Hypeiploidy induced by drugs that inhibit formation of nacrotubule promotes chromosome instability."Genes to Cell. 7(2). 151-162 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura T et al.: "Intratumoral delivery of interleukin 12 expression plasmids with in vivo electroporation is effective for colon and renal cancer."Human Gene Therapy. 12(10). 1265-1276 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Goto T et al.: "Highly efficient electro-gene therapy of solid tumor by using an expression plasmid for the herpes simplex virus thymidine kinase gene."Proc Natl Acad Sci USA. Jan 497(1). 354-359 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kimura: "Expression of lymphocyte-specific chemokines in human malignant glioma : Essential role of LARC in cellular immunity of malignant glioma"International Journal of Oncology. 21(4). 707-715 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shiraishi S: "Influence of p53 mutations on prognosis of patients with glioblastoma"Cancer. 95(2). 249-257 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kamiryo T: "Analysis of homozygous deletion of the p16 gene and correlation with survival in patients with glioblastoma multiforme"Journal of Neurosurgery. 96(5). 815-822 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nitta M: "Hyperploidy induced by drugs that inhibit formation of microtubule promotes chromosome instability"Genes to Cell. 7(2). 151-162 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tada K: "Analysis of loss of heterozygosity on chromosome 10 in patients with malignant astrocytic tumors : correlation with patient age and survival"Journal of Neurosurgery. 95(4). 651-659 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tamura T: "Intratumoral delivery of interleukin 12 expression plasmids with in vivo electroporation is effective for colon and renal cancer"Human Gene Therapy. 12(10). 1265-1276 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kino: "Identification of the cis-acting region in the NF2 gene promoter as a potential target for mutation and methylation-dependent silencing in schwannoma"Genes to cells. 6. 441-454 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Goto: "Suprasellar Hemangioblastoma in a patient with von hippel-lindau disease confirmed by germline mutation study"Surgical Neurology. 56. 22-26 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Tamura: "Intratumoral Delivery of Interleukin 12 Expression Plasmids with In Vivo Electroporation Is Effective for Colon and Renall Cancer"Humn Gene Therapy. 12. 1265-1276 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Takeshima: "Metastatic Brain Tumours from Oesophageal Carcinoma : Neuro-Imaging and Clinicopathological Characteristics in Japanese Patients"Acta Neurochirurgica(Wien). 143. 31-36 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Murakami: "The invasive features of glial and non-central nervous system tumor cells are different on organotypic brain slices from newborn rats"International Journal of Oncology. 18. 721-727 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Murakami: "The inhibitory effect of simvastatin on growth in malignant gliomas -with special reference to its local application with fibrin glue spray in vivo"International Journal of Oncology. 19. 525-531 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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