| Project/Area Number |
13557121
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | KYOTO PREFECTURAL UNIVERSITY OF MEDICINE |
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Principal Investigator |
IMAHORI Yoshio KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (80191899)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJINO Hitoshi KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (00347452)
SASAHIMA Hiroyasu KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 講師 (80196188)
MINEURA Katsuyoshi KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, PROFESSOR, 医学部, 教授 (70134103)
ONO Kouji KYOTO UNIVERSITY, KURRI, PROFESSOR, 医学部, 助教授 (90122407)
TAKAHASHI Yoshinobu KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (90347451)
橋本 直哉 京都府立医科大学, 医学部, 助手 (90315945)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2002: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2001: ¥9,600,000 (Direct Cost: ¥9,600,000)
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| Keywords | Glioma / mRNA. / positron emission tomography / antisense / oligodeoxynecleotide / glial fibrillary acidic protein / ethylketene / in situ hybridization / 遺伝子発現 / PET / テロメラーセ゜ / 遺伝子診断 / 遺伝子治療 / VEGF |
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| Research Abstract |
Tumor angiogenesis plays an important role in prolifereation and invasion. Expression of the mRNAs of vascular endothelial growth factor (VEGF) and their receptors accelerate tumor proliferation together with tumor angiogenesis. In these conditions, it is a matter of great importance for therapy to know how to advance angiogenesis and where dose it develops in malignant tumor. Also an elucidate of expression of the mRNAs in vivo is a problem to be solved. In the present study we attempted an imaging of expression of Mrna by positron emission tomography (PET) with positron labeled antisense oligo deoxynucleic acid (ODN). In experiment using rats, DNA-sequence dependent images of glial fibrillary acidic protein (GFAP) and telomerase Mrna expression were successfully obtained. Positron labeled antisense ODN against VEGF is widely applicable to cancer therapy, and we studied in situ hybridization using the ODN-antisense in human brain tumor samples. This result suggested the possibility for visualization of the expression of VEGF Mrna. In conclusion this study demonstrated that visualization of the expression of Mrna.s in vivo is possible based on DNA sequence manner by positron labeling ODN antisense method. This new technique can contribute powerful strategic cancer therapy in the melecular level
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