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Biological control for the rate of orthodontic tooth movement and root resorption

Research Project

Project/Area Number 13557183
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 矯正・小児・社会系歯学
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

TAKANO Teruko  Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (00127250)

Co-Investigator(Kenkyū-buntansha) YOSHIKO Tsuboi  Okayama University Hospital of Dentistry, Research assistant, 歯学部附属病院, 助手 (50325122)
樋口 義信  中外製薬株式会社, 創薬第二研究所, 主査(研究職)
IWAMOTO Masahiro  Osaka University Graduate School of Dentistry, Assistant professor, 大学院・歯学研究科, 講師 (30223431)
TATSUYA Fujiki  Okayama University, Hospital of Dentistry Research assistant, 歯学部附属病院, 助手 (20322232)
YOSHINOBU Higuchi  Chugai Pharmaceutical Co. Ltd., Researcher
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥8,300,000 (Direct Cost: ¥8,300,000)
KeywordsBiology / Experimental tooth movement / Anchorage / Gene delivery / CTGF / β-galactoshidase gene / 保定 / β-galactosidase遺伝子 / 1,25(OH)_2D_3 / 24,25(OH)_2D_3 / TGF-β1
Research Abstract

Orthodontic tooth movement depends on the mechanical stress onto the tooth, but there are various problems, such as root resorption, long treatment period, pain, undesirable oral hygiene due to the multibracket system and others. Therefore, the biological and physiological control for the tooth movement and the anchorage control, not depending only on the mechanical stress should be established.
1. Trying to suppress the relapse of the tooth after experimental tooth movement, we locally injected 1,25(OH)_2D_3 or TGF-β in the pressure side of the alveolar bone. 1,25(OH)_2D_3, injected group had no effect on the relapse. On the other hand, 24,25(OH)_2D_3 or TGF-β delivered group suppressed the tooth relapse showing few osteoclasts, but active bone formation by osteoblasts. Furthermore, in these groups the bone volume and bone formation surface in the pressure side increased, and resorption surface and the number of osteoclasts decreased.
2. HVJ liposome method is developed an efficient and … More nontoxic in vivo gene transfer compared to adenoviral vector-mediated gene delivery. Following experiments were performed to examine the optimum conditions of HVJ lipoeome method for experimental tooth movement model in rats.
(1) Luciferase gene was delivered to mesenchymal cells, isolated from bone marrow in mouse femur, by using HVJ-liposome method in vitro. Luciferase reporter assay was performed to evaluate delivery rate.
(2) During experimental tooth movement in rats, β-galactoeidase gene (LacZ) was delivered to the maxillae by using HVJ-liposome method. Three days after gene introduction, X-gal staining was performed to evaluate the deliveryate. These studies indicated that the gene delivery by using HVJ-liposome was efficient to the experimental tooth movement.
3. Experimental tooth movement was performed after CTGF gene was delivered by using HVJ-liposome in rat. After 1,3,5,7 and 15 days of tooth movement, the maxillary bones were dissected. In situ hybridization and immunohistochtemistry were performed to analyze the expression of CTGF in periodontal tissue. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Yoshimichi, G.: "CTGF/Hcs24 interacts with the cytoskeletal protein actin in chondrocytes"Biochem Biophys Res Commun. 299. 755-761 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwamoto, M.: "The role of ERG (ets related gene) in cartilate development"Osteoarthritis Cartilage. 9 Suppl A. S41-S47 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro, T.: "Activation of the Bulbospinal Serotonergic System during Experimental Tooth Movement in the Rat"Journal of Dental Research. 80. 1854-1857 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro, T.: "Influences of ovariectomy on experimental tooth movement in the rat"Journal of Dental Research. 80. 1858-1861 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuboi, Y.: "Mitogenic effects of neurotrophins on periodontal ligament cell line"Journal of Dental Research. 80. 881-886 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro, T.: "Inferior alveolar nerve transection inhibits increase in osteoclast appearance"Bone. 26. 663-669 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Takano-Yamamoto (Ed.K.Takada, William R.Proffit): "Orthodontics in the 21^<st> Century -Where are We Now? Where Are We Going?"Osaka University Press. 216 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yosimilchi, G: "CTGF/Hcs24 interacts with the cytoskeletal protein actin in chondrocytes"Biochem Biophys Res Commun.. 299(5). 755-761 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwamoto, M: "The role of ERG (ets related gene) in cartilate development"Osteoarthritis Cartilage. 9 Suppl A. S41-S47 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro, T: "Activation of the Bulbospinal Serotonergic System during Experimental Tooth Movement in the Rat"Journal of Dental Research. 80. 1854-1857 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro, T: "Influences of ovariectomy on experimental tooth movement in the rat"Journal of Dental Research. 80. 1858-1861 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuboi, Y: "Mitogenic effects of neurotrophins on periodontal ligament cell line"Journal of Dental Research. 80. 881-886 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro, T: "Inferior alveolar nerve transection inhibits increase in osteoclast appearance"Bone. 26. 663-669 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Takano-Yamamoto (Ed.K. Takada and William R. Profit): "Orthodontics in the 21^<st> Century-Where are We Now? Where Are We Going? Osaka University Press"Introductory Remarks: Current views of molecular biology in Orthodontics. 81-88 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Miyatake, E.: "Mandibular prognathism with transverse maxillary deficiency and skeletal asymmetry treated by segmental high LeFoft I osteotomy and IVRO"Orthodontic Waves. 61(3). 184-190 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yosimichi G: "CTGF/Hcs24 interacts with the cytoskeletal protein actin in chondrocytes"Biochem Biophys Res Commun. 299(5). 755-761 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Deguchi, T.: "Miniature implants for orthodontic anchorage"J Dent Res. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Eri MIYATAKE: "Angle Class III case with severe facial asymmetry, unilateral posterior crossbite and temporamandibular disorders treated by LeFort I osteotomy"American Journal of Orthodontics and Dentofacial Orthodontics. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Deguchi, T.: "Increase in number of Galanin-immunoreactive nerve fibers in the periodontal ligament during experimental tooth movement in rats"J Dent Res. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] N.H Moritani: "Interaction of AP-1 and the ctgf gene"JBMM. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Takano-Yamamoto: "Orthodontics in the 21^<st> Century-Where are We Now? Where Are We Going? (Ed.K.Takada and William R Proffit)"Osaka University Press. 216 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamashiro, T.: "Mechanical stimulation induces CTGF expression in rat osteocytes"Journal of Dental Research. 461-465 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kamioka, H.: "A three-dimensioned distribution of osteocyte processes revealed by the combination of CLS and DIC microscopy"Bone. 28・2. 145-149 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashiro, T.: "Epithelial rests of Malassez expressed immunoreactivity of TrkA and its distribution is regulated by sensory nerve innervation"Journal of Histochemistry and Cytochemistry. 48・7. 979-984 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashiro, T.: "Medullary monoamine levels during experimental tooth movement"Brain Research. 878. 199-203 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuboi, Y.: "Mitogenic effects of neurotrophins on periodontal ligament cell line"Journal of Dental Research. 80・3. 881-886 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashiro, T.: "Inferior alveolar nerve transection inhibits increase in osteoclast appearance"Bone. 26・6. 663-669 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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