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New Strategy for Periodontal Treatment Based on Control of Angiogenesis in Periodontal Tissues using VEGF and Anti-VEGF.

Research Project

Project/Area Number 13557191
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Periodontal dentistry
Research InstitutionKagoshima University

Principal Investigator

IZUMI Yuichi  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (60159803)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Matsuo  Kagoshima University, Research Center for Life Science Resources, Associate Professor, 生命科学資源開発研究センター, 助教授 (50332896)
FURUICHI Yasushi  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (80305143)
TORII Mitsuo  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (30116066)
MATSUO Katsuhiko  Toagosei Co., Ltd, Bioscience Research Department, Chief Researcher, バイオサイエンス研究部, 主任研究員
松下 健二  鹿児島大学, 歯学部, 助手 (90253898)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsPeriodontal disease / Vascular endothelial growth factor / Gingival fibroblasts / Periodontal ligament fibroblasts / Periodontal pathogens / Gingival crevicular fluid / Periodontal regeneration / ポリ乳酸 / マイクロカプセル / 歯周組織再生 / 歯周病関連細菌
Research Abstract

Vascular endothelial growth factor (VEGF) has recently attracted attention as a potent inducer of vascular permeability and angiogenesis. To clarify the role of VEGF in periodontal lesion, we investigated 1) the-properties of VEGF expression in the cells derived from human periodontal tissues in culture, 2) the detection of the changing levels of VEGF in gingival crevicular fluid (GCF) following two months of scaling and root planning (SRP), and 3) the effects of VEGF on human periodontal ligament fibroblasts (HPLF) culture through the induction of osteogenic markers and nodule formation. The results showed that 1) human gingival fibroblasts (HGF) constitutively produced VEGF and levels were significantly enhanced by stimulation with Ve and OMP from A.actinomycetemcomitans and P.gingivalis. Supernatant from cultures of Ve-and OMP-stimulated HGF strongly induced vascular permeability enhancement, and 2) that total amounts of VEGF in GCF might indicate the severity of periodontitis and t … More he concentration of VEGF could predict the healing response of periodontal tissues following SRP. These results suggest that VEGF may be associated with the etiology of periodontitis in its early stages according to neovascularization stimulated by periodontal pathogens causing swelling and edema. Furthermore, the results revealed 3) that VEGF enhanced the expression of c-fos, osteopontin, osteocalcin, bone morphogenesis protein-2 and periodontal ligament associated protein-1 in HPLF culture by stimulation with VEGF^<121> and VEGF^<165>, but not in HGF. VEGF^<165> induced these osteogenic markers more strongly than VEGF^<121>. The alkaline phosphatase activity and calcium concentration were up-regulated after HPLF culture was stimulated with VEGF^<121> and VEGF^<165> for 15 days, while nodule formations also up-regulated after 21 days. These results suggest that HPLF has a phenotype similar to osteoblasts after being stimulated with VEGF, and VEGF may have an important role in initiating the process of periodontal tissue healing. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] K.Suthin: "Possible roles of VEGF in periodontal tissue : Expression of VEGF in human gingival and periodontal ligament fibroblasts and its effects on the cells in culture"J.Hard Tissue Biology. 10. 85-88 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 石川久美子: "歯周病患者歯肉溝滲出液中に検出されるvascular endothelial growth factor (VEGF)とその変動"日歯周誌. 44. 387-395 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 山本松男: "歯の喪失と骨粗鬆症"CLINICAL CALCIUM. 12. 987-991 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Suthin: "Enhanced expression of vascular endothelial growth factor by periodontal pathogens in gingival fibroblasts"J.Periodont.Res.. 38. 90-96 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Yamamoto: "Effects of Recombinant human growth/differentiation factor-5 using in vitro and in vivo experiments"Dentistry in Japan. 39. 119-122 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 山本松男: "スタチンの歯周病治療薬としての可能性"The Quintessence. 22. 1965-1969 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakamura: "Effects of growth/differentiation factor-5 on human periodontal ligament cells"J Periodont Res. 38. 597-605 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Suthin: "Possible roles of VEGF in periodontal tissue : Expression of VEGF in human gingival and periodontal ligament fibroblasts and its effects on the cells in culture"J.Hard Tissue Biology. 10. 85-88 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Ishikawa: "The level of vascular endothelial growth factor in gingival crevicular fluid of periodontitis patients."J.Jpn.Soc.Periodontol.. 44. 387-395 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Yamamoto: "Tooth loss and osteoporosis."Clinical Calcium. 12. 987-991 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Suthin: "Enhanced expression of vascular endothelial growth factor by periodontal pathogens in gingival fibroblasts"J.Periodont.Res.. 38. 90-96 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Yamamoto: "Effects of Recombinant human growth/differentiation factor-5 using in vitro and in vivo experiments"Dentistry in Japan. 39. 119-122 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Yamamoto: "Statin as a possible drug for periodontal therapy."The Quintessence. 22. 1965-1969 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakamura: "Effects of growth/differentiation factor-5 on human periodontal ligament cells"J Periodont Res. 38. 597-605 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsuo Yamamoto: "Effects of recombinant human growth/differentiation factor-5 using in vitro and in vivo experiments."Dentistry in Japan. Vol.39. 119-122 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toshiaki Nakamura: "Effects of growth/differentiation factor-5 on human periodontal ligament cells."Journal of Periodontal Research. Vol.38・6. 597-605 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 石川 久美子: "歯周病患者歯肉溝滲出液中に検出されるvascular endothelial growth factor(VEGF)とその変動"日本歯周病学会会誌. 44・4. 387-395 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanyarat Suthin: "Enhanced expression of vascular endothelial growth factor by periodontal pathogens in gingival fibroblasts"Journal of Periodontal Research. 38・1. 90-96 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanyarat Suthin: "Possible roles of VEGF in periodontal tissue : Expression of VEGF in human gingiva and periodontal ligament fibroblasts and its effects on cells in culture"Journal of Hard Tissue Biology. 10・2. 85-88 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanyarat Suthin: "Enhanced expression of vascular endothelial growth factor by periodontal pathogens in gingival fibroblasts"Journal of Periodontal Research. (in press).

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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