| Project/Area Number |
13557205
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biological pharmacy
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
OZAWA Koichiro Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (10211822)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHII Michiko Hiroshima University, Faculty of Medicine, Teaching Associate, 医学部, 教務員 (40230677)
ISHIBARA Kumatoshi Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (20212912)
MASUJIMA Tsutomu Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (10136054)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | infrared laser-spectromicroscope / histamine / mast cells / RBL-2H3 cells / exocytosis / pharmacology / clinical pharmacy / muscle atrophy / 薬効解析 |
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| Research Abstract |
We have developed a new type of infrared laser spectromicroscope to analyze the dynamics of microorgans in living cells/tissues and to clarify the secretory mechanisms of granules which contain hormones and transmitters in living cells/tissues. In this study, simultaneous analysis of exocytosis of histamine-containing secretory granules and concentration of intracellular Ca^<2+> ([Ca^<2+>]i) with the microscope was used to characterize the antigen-induced Ca^<2+> responses and exocytosis of single fluo-3-loaded rat basophilic leukemia (RBL-2H3) cells in the presence and absence ** extracellular Ca^<2+>. Our results suggest that increase of [Ca^<2+>]I is not sufficient for exocytosis stimulated with antigen, but release of Ca^<2+> from intracellular stores induces Ca^<2+>-influx, followed by exocytosis in RBL-2H3 cells.
We also investigated mechanisms underlying experimentally-induced atrophy of skeletal muscle using the infrared laser-spectromicroscope. The hind limbs of hamsters in a hypothermic group (8 to 12℃)retained more muscle wet-weight and myofibers cross-sectional area in the soleus and gastrocnemius muscles compared with the normothermic group (23 to 25℃), which experienced a significant. Our results indicate that a hypothermic environment inhibits short-term muscle atrophy. This inhibition may be caused by the increased caloric intake combined with a state similar to hibernation in low-temperature environments.
These methods would be useful not only for investigating the detailed relationship between intracellular signaling mechanisms and intracellular calcium mobilization in RBL-2H3 cells, but also for studying the signaling mechanisms in many kinds of cells and for clinical pharmacy.
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