| Project/Area Number |
13557226
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Laboratory medicine
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| Research Institution | Mie University |
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Principal Investigator |
SHIRAISHI Taizo Mie University, Faculty of Medicine, Professor, 医学部, 教授 (30162762)
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| Co-Investigator(Kenkyū-buntansha) |
NOBORI Tsutomu Mie University, Faculty of Medicine, Professor, 医学部, 教授 (60106995)
KOMADA Yoshihiro Mie University, Faculty of Medicine, Professor, 医学部, 教授 (80186791)
TAKAO Motoshi Mie University, Faculty of Medicine, Reseach Associate, 医学部, 助手 (30263007)
YAMAMORI Syunji Mitsubishikagaku BCL, Gene Analysis, Director, 遺伝子解析部, 部長(研究職)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2003: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | MTAP gene deficiency / Lung Cancer / Leukemia / Chemotherapy / PCR / Immunohistochemistry / MTAP / promoterのメチル化 / 非小細胞肺癌 / 定量的PCR法 / モノクロナール抗体 / 抗癌剤感受性試験 / Methylthioadenosine |
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| Research Abstract |
Materials were obtained with permission from patients who underwent operations for lung cancer. MTAP genetic analysis was carried out on 78 samples. IHC was carried out on 72 samples. MTAP gene was analyzed by real-time PCR while MTAP protein was detected by IHC. Methylation specific primers were used to analyze the promoter methylation MTAP gene on DNA treated with sodium bisulfate. 24 (32.9%) out of 73 samples were found to lack the MTAP gene. IHC was performed on 72 samples, since 6 samples were too small for evalucation with IHC. Out of 72 samples, 32 (44.4%) were found to be MTAP deficient with IHC. Of 47 samples tested positive with real-time PCR, 40 were MTAP positive but seven were negative with IHC. Twenty-three samples were MTAP negative with both real-time PCR and IHC. All 7 samples tested positive with real-time PCR, but negative with IHC were found to have the hypermethylated MTAP promoter, consisting of 23.3% in MTAP deficiency diagnosed with IHC. The reliability of the IHC-based diagnosis for MTAP deficiency was confirmed. This IHC method is applicable to formalin-fixed specimens. IHC was found to be specific enough to choose candidates for the selective chemotherapy targeting MTAP deficiency.
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