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Study on cardio-creation using vessels circumvalated by fetal or ES cell-derived cardiac tissues and their application for cardiac diseases

Research Project

Project/Area Number 13558107
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionShinshu University

Principal Investigator

AMANO Jun  Shinshu University, School of Medicine, professor, 医学部, 教授 (30170666)

Co-Investigator(Kenkyū-buntansha) JOHKURA Kohei  Shinshu University, School of Medicine, Assistant Professor, 医学部, 講師 (30303473)
TAGAWA Yo-ichi  Shinshu University, Research center for human and environmental sciences, Associate Professor, ヒト環境科学研究支援センター, 助教授 (70262079)
AMANO Jun  Shinshu University, School of Medicine, professor, 医学部, 教授 (20138283)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥10,000,000 (Direct Cost: ¥10,000,000)
Keywordscreation of the heart / fetal cardiocytes / ES cell-derived cardiocytes / cardiocyte transplantation / vessels / partial cardiac transplantation / implanted lung-heart apparatus / ES細胞 / 動脈の心臓化 / 静脈の心臓化 / IS細胞
Research Abstract

This research project has demonstrated the possibility of fetal cardiac tissues and ES cell derived cardiocytes adding spontaneous contraction to vessels by their ectopic transplantation around vascular tubes and its application for cardiac diseases.
1.Mouse fetal cardiac tissues were transplanted around the abdominal aorta of the nude mouse. They survived securing blood supply from the testicular artery and showed spontaneous contraction and action potential. Morphological analysis confirmed that the contracted tissues contained cardiocytes. This method will be applied for implanted ventricular assist system.
2.ES cell-derived cardiocytes were differentiated from ES cells by hanging drop method or a new method using conical tubes and transplanted around the abdominal aorta of the nude mouse. On and after 7 days spontaneous contracted tissues were recognized on the aorta and generated irregular action potentials. Morphological and molecular biological analysis showed these tissues including atrial and ventricular cardiocytes. Moreover, it was confirmed that atrial cardiocytes contained atrial natriuretic peptide by RT-PCR and immunohistochemistry.
3.To create a new type of implanted lung-heart apparatus, TiO_2 nanotubes with photodecomposition of water into O_2 and H_2 as an Oxygen supplier and fetal cardiac tissues as a pump were implanted under the inguinal region of the nude mouse. After 4 weeks, Sv02 of the surface of the treated skin were measured, showing higher percentage than controls. Transplanted cardiac tissues contracted spontaneously and showed features of cardiocytes ultrastructures.
4.The surgical method for partial transplantation using cardiac tissues was developed. It was featured by transverse incision of the chest through the intercostals space and sternum and offered the wide exposure for treatment. A part of the greater omentum containing cardiac tissues were sutured easily to the wall of the original heart by this method.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Johkura K, Cui L, Suzuki A, Teng R, Kamiyoshi A, Okamura S, Kubota S, Zhao X, Asanuma K, Okouchi Y, Ogiwara N, Tagawa Y, Sasaki K: "Survival and function of mouse embryonic stem cell-derived cardiomyocytes in ectopic transplants."Cardiovasc Res. 58. 435-443 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iida I, Johkura K, Teng R, Kubota S, Cui L, Zhao X, Ogiwara N, Okouchi Y, Asanuma K, Nakayama J, Sasaki K: "Immunohistochemical localization of hepatocyte growth factor activator (HGFA) in developing mouse liver tissues : heterogeneous distribution of HGFA protein."J Histochem Cytochem. 51. 1139-1149 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koganehira Y, Takeoka M, Sasaki K, Murata K, Saida T, Taniguchi S: "Reduced expression of actin-binding proteins, h-caldesmon and calponin h1, in the vascular smooth muscle inside melanoma lesions : an adverse prognostic factor for malignant melanoma."British J Dermatol. 148. 971-980 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Teng R, Johkura K, Ogiwara N, Zhao X, Cui L, Iida I, Okouchi Y, Asanuma K, Sasaki K: "Morphological analysis of leukocyte tranemigration in the pleural cavity."J Anat. 203. 391-404 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurosawa H, Imamura T, Koike M, Sasaki K, Amano Y.: "A simple method for forming embryoid body from mouse embryonic stem cell."J Biosci Bioeng. 96. 409-411 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cui L, Johkura K, Liang Y, Teng R, Ogiwara N, Okouchi Y, Asanuma K, Sasaki K: "The biodefense function of the mental milky spots through the cell adhesion molecules and leukocyte proliferation."Cell Tissue Res. 310. 321-330 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 佐々木克典, 城倉浩平他 訳(井上貴央監訳): "カラー人体解剖学 構造と機能:ミクロからマクロまで"西村書店. 643 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Johkura K, Cui L, Suzuki A, Teng R, Kamiyoshi A, Okamura S, Kubota S, Zhao X, Asanuma K, Okouchi Y, Ogiwara N, Tagawa Y, Sasaki K: "Survival and function of mouse embryonic stem cell-derived cardiomyocytes in ectopic transplants."Cardiovase Res. 58. 435-443 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iida I, Johkura K, Teng R, Kubota S, Cui L, Zhao X, Ogiwara N, Okouchi Y, Asanuma K, Nakayama J, Sasaki K: "Immunohistochemical localization of hepatocyte growth factor activator (HGFA) in developing mouse liver tissues : heterogeneous distribution of HGFA protein."J Histochem Cytochem. 51. 1139-1149 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koganehira Y, Takeoka M, Sasaki K, Murata K, Saida T, Taniguchi S: "Reduced expression of actin-binding proteins, h-caldesmon and calponin h1, in the vascular smooth muscle inside melanoma lesions : an adverse prognostic factor for malignant melanoma."British J Dermatol. 148. 971-980 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Teng R, Johkura K, Ogiwara N, Zhao X, Cui L, Iida I, Okouchi Y, Asanuma K, Sasaki K: "Morphological analysis of leukocyte transmigration in the pleural cavity."J Anat. 203. 391-404 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ilic D, Kovaci B, Johkura K, Schlaepfer DD, Tomasevi N, Han Q, Kim J-B, Howerton K, Baumbusch C, Ogiwara N, Streblow DN, Nelson JA, Dazin P, Shino Y, Sasaki K, Damsky CH.: "FAK promotes organization of fibronectin matrix and fibrillar adhesions."J Cell Sci. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kurosawa H, Imamura T, Koike M, Sasaki K, Amano Y.: "A simple method for forming embryoid body from mouse embryonic stem cell."J Biosci Bioeng. 96. 409-411 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Liang Y, Nakamura S, Cui L, Johkura K, Ogiwara N, Sasaki K: "Three-dimensional expression of adhesion molecules on the superficial synovial intima of the LPS-induced arthritis of the mouse knee analyzed by immuno-SEM."J Electron Microsc. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kubota S, Johkura K, Asanuma K, Okouchi Y, Ogiwara N, Sasaki K, Kasuga T.: "Titanium Oxide nanotyu-bes for bone regeneration."J Materials Science : Materials in Medicine. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cui L, Johkura K, Yue F, Ogiwara N, Okouchi Y, Asanuma K, Sasaki K: "Spatial distribution of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells, and its change upon differentiation."J Histochem Cytochem. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cui L, Johkura K, Liang Y, Teng R, Ogiwara N, Okouchi Y, Asanuma K, Sasaki K: "The biodefense function of the mental milky spots through the cell adhesion molecules and leukocyte proliferation"Cell Tissue Res. 310. 321-330 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okamura S, Suzuki A, Johkura K, Liang Y, Ogiwara N, Nakazawa A, Harigaya M, Yokouchi T, Sasaki K: "The formation of the bio-pulsatile vascular pump by cardiomyocyte transplants circumvallanting the abdominal aorta."Tissue Eng. 8. 201-211 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Johkura K, Liang Y, Teng R, Ogiwara N, Sasaki K: "Nephrogenesis accopanied by vascularization in the mouse embryonic metanehros transplanted into the adult kidney for the creation of additional nephros."Nephrology. 7. 86-94 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inuma H, maruyama K, Okinaga K, Sasaki K, Sekine T, Ishida O, Ogiwara N, Johkura K, Yonemura Y: "Intracellular targeting therapy of cisplatin-encapsulated transferring-polyethylene glycol liposome on peritoneal dissemination of gastric cancer."Int.J.Cancer. 99. 130-137 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Johkura K, Liang Y, Cui L, Ogiwara N, Sasaki K: "Spatial distribution of cell adhesion molecules on the peritoneal surface in the cecal perforation-induced peritonitis."Anat Rec. 264. 219-227 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Johkura K, Cui L, Suzuki A, Teng R, Kamiyoshi A, Okamura S, Kubota S, Zhao X, Asanuma K, Okouchi Y, Ogiwara N, Tagawa Y, Sasaki K: "Survival and function of mouse embryonic stem cell-derived cardiomyocytes in ectopic transplants"Cardiovasc Res. 58. 435-443 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iida I, Johkura K, Teng R, Kubota S, Cui L, Zhao X, Ogiwara N, Okouchi Y, Asanuma K, Nakayama J, Sasaki K: "Immunohistochemical localization of hepatocyte growth factor activator (HGFA) in developing mouse liver tissues : heterogeneous distribution of HGFA protein"J Histochem Cytochem. 51. 1139-1149 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Teng R, Johkura K, Ogiwara N, Zhao X, Cui L, Iida I, Okouchi Y, Asanuma K, Sasaki K: "Morphological analysis of leukocyte transmigration in the pleural cavity"J Anat. 203. 391-404 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kurosawa H, Imamura T, Koike M, Sasaki K, Amano Y.: "A simple method for forming embryoid body from mouse embryonic stem cell"J Biosci Bioeng. 96. 409-411 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Koganehira Y, Takeoka M, Sasaki K, Murata K, Saida T, Taniguchi S: "Reduced expression of actin-binding proteins, h-caldesmon and calponin hl, in the vascular smooth muscle inside melanoma lesions : an adverse prognostic factor for malignant melanoma"British J Dermatol. 148. 971-980 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okamura S, Suzuki A, Johkura K, Liang Y, Ogiwara N, Nakazawa A, Harigaya M, Yokouchi T, Sasaki K.: "The formation of the bio-pulsatile vascular pump by cardiomyocyte transplants circumvallanting the abdominal aorta"Tissue Eng. 8. 201-211 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐々木 克典, 城倉 浩平他 訳(井上 貴央監訳): "カラー人体解剖学 構造と機能:ミクロからマクロまで"西村書店. 643 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Johkura K, Cui L, Suzuki A, Teng R, Kamiyoshi A, Okamura S, Kubota S, Asanuma K, Okouchi Y, Ogiwara N, Tagawa Y, Sasaki K: "Survival and function of mouse embryonic stem cell-derived cardiomyocytes in ectopic transplants"Cardiovascular Res. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Cui L, Johkura K, Liang Y, Teng R, Ogiwara N, Okouchi Y, Asanuma K, Sasaki K.: "The biodefense function of the mental milky spots through the cell adhesion molecules and leukocyte proliferation"Cell Tissue Res. 310. 321-330 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okamura S, Suzuki A, Johkura K, Liang Y, Ogiwara N, Nakazawa A, Harigaya M, Yokouchi T, Sasaki K: "The formation of the bio-pulsatile vascular pump by cardiomyocyte transplants circumvallanting the abdominal aorta"Tissue Eng. 8. 201-211 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Johkura K, Liang Y, Teng R, Ogiwara N, Sasaki K: "Nephrogenesis accopanied by vascularization in the mouse embryonic metanephros transplanted into the adult kidney for the creation of additional nephros"Nephrology. 7. 86-94 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Johkura K, Liang Y, Cui L, Ogiwara N, Sasaki K.: "Spatial distribution of cell adhesion molecules on the peritoneal surface in the cecal perforation-induced peritonitis"Anat Rec. 264. 219-227 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sasaki K, Johkura K, Ogiwara N, Liang Y, Cui L, Teng R, Okouchi Y, Asanuma K, Ishida O, Maruyama K: "Three-dimensional morphological analysis of antigen-antibody reaction in hepatic sinusoids preserved in hypothermic UW"Cryobiology. 42. 145-150 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okamura S, Suzuki K, Johkura K, Ogiwara M, Nakazawa A, Harigaya M, Yokouchi T, Sasaki K.: "Formation of bio-pulsatile vascular pump by cardiomyocyte transplant circumvallating the abdominal aorta"Tissue Eng.. 8(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Johkura K, Liang Y, Teng R, Ogiwara N, Sasaki K.: "Neplirogenesis accompanied by vascularisation in the mouse embryonic metanephios transplanted into the adult kidney for the creation of additional nephros"Nephrology. 7. 86-94 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Johkura K, Liang Y, Cui L, Ogiwara N, Sasaki, K: "Spatial distribution of cell adhesion molecules on the peritoneal surface in the cecal perforation-induced peritonitis"Anat. Rec.. 264. 219-227 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sasaki K, Johkura K, Ogiwara N, Lianf Y, Cui L, Teng R, Okouchi Y.: "Three-dimensional morphological analysis of antigen-antibody reaction in hepatic sinusoids preserved in hypothermic UW solution"Cryobiology. 42. 145-150 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Liang Y, Jolikura K, Ogiwara N, Sasaki K: "Expression of adhesion molecules and fibronectin of activated peritoneal surface with lipopolysaccharide(LPS) analyzed with immuno SEM"Ann. Anat.. 183. 353-356 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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