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Development of UV excitation CARS microscopy system and applications to biological tissue measurement

Research Project

Project/Area Number 13558112
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University

Principal Investigator

HASHIMOTO Mamoru  Osaka University, Graduate School of engineering science, Associate Professor, 大学院・基礎工学研究科, 助教授 (70237949)

Co-Investigator(Kenkyū-buntansha) CHIKAMORI Kensuke  Natuto University of education, Department of Basic Human Science for Integrated Studies, Associate Professor, 学校教育学部, 助教授 (40108874)
YASUI Takeshi  Osaka University, Graduate School of engineering science, Research Associate, 大学院・基礎工学研究科, 助手 (70314408)
ARAKI Tsutomu  Osaka University, Graduate School of engineering science, Professor, 大学院・基礎工学研究科, 教授 (50136214)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2002: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥11,300,000 (Direct Cost: ¥11,300,000)
Keywordsmulti-photon microscopy / Raman Spectroscopy / CARS / 3D imaging / Molecular Structure / Nonlinear Optics / Biomedical measurements / キーワード7 / キーワード8
Research Abstract

We developed a multi-focus CARS microscopy system using two synchronized ps-Ti : Sapphire lasers. CARS microscope require two lasers with different wavelength, high peek intensity, narrow spectral bandwidth around a few cm^<-1>, and large wavelength scanning range about 2000cm^<-1>. Therefore, we developed a system using two synchronized ps-Ti : Sapphire lasers for light source. A micro-lens array is used to excite and observe parallel points for shortening the measurement time. The second harmonics of lasers are also available to use for electric resonance enhancement.
For the polystyrene beads without absorption at near-IR and UV, the CARS spectrum and image are obtained by near-IR excitation only, because of low intensities of UV beam. On the other hand for human erythrocyte which has absorption around 400 nm, Clear CARS spectrum and image are obtained by UV excitation, because of electric resonance enhancement.
As the results, it was confirmed that the electric resonance enhancement is the important for CARS microscopy.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Mamoru Hashimoto: "Coherent Anti-Stokes Raman Scattering Microscopy"Acta Histoehem. Cytochem.. 35. 83-86 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 橋本守: "CARS顕微鏡"電子顕微鏡. 37. 55-58 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 橋本守, 荒木勉, 河田聡: "コヒーレントアンチストークスラマン散乱を用いた顕微鏡"電子情報通信学会技術研究報告. 102・387. 25-28 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 橋本 守: "コヒーレントアンチストークスラマン散乱顕微鏡の開発"レーザー研究. 31(印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 橋本 守(分担執筆): "ナノテクノロジーハンドブック"オーム社(印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mamoru Hashimoto: "Coherent Anti-Stokes Raman Scattering Microscopy"Acta Histehem. Cytochem.. Vol.35. 83-86 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mamoru Hashimoto: "CARS microscopy"Electron Microscopy. Vol37. 55-58 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mamoru Hashimoto: "New microscope using coherentanti-Stokes Raman spectroscopy"JEICE Technical Report. Vol.102. 25-28 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mamoru Hashimoto: "Development of coherent anti-Stokes Raman scattering microscope"The Review of Laser Engineering. Vol.31(in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mamoru Hashimoto: "Handbook of nano technology"Ohmusha. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mamoru Hashimoto: "Coherent Anti-Stokes Raman Scattering Microscopy"Acta Histochem. Cytochem.. 35. 83-86 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 橋本守: "CARS顕微鏡"電子顕微鏡. 37. 55-58 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 橋本守, 荒木勉, 河田聡: "コヒーレントアンチストークスラマン散乱を用いた顕微鏡"電子情報通信学会技術研究報告. 25-28 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 橋本 守: "コヒーレントアンチストークスラマン散乱顕微鏡の開発"レーザー研究. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Hashimoto: "CARS microscopy in the fingerprint region"Abstructs of multi-dimensiona microscopy 2001. 22 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 橋本守: "生命現象をありのままに捉える-CARS顕微鏡-"平成13年度日本電子顕微鏡学会ニューマイクロスコープ研究分科会講演論文集. 25-29 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 橋本守: "コヒーレントアンチストークスラマン散乱顕微鏡"光学. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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