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Development of the real time three-dimensional confocal microscopic system

Research Project

Project/Area Number 13558117
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionTokai University

Principal Investigator

ISHIDA Hideyuki  Tokai University, School of Medicine, Associate Professor, 医学部, 助教授 (20222424)

Co-Investigator(Kenkyū-buntansha) GENKA Chokoh  Tokai University, School of Medicine, Assistant Professor, 医学部, 講師 (30317820)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordsthree-dimention / confocal / calcium / Nipkow / real-time
Research Abstract

By the development of confocal microscope with the resolution in depth direction, the biological response in intracellular local field is clarified, and it is reported in great numbers. However, it is disadvantageous to be difficult to obtain information of whole intracellular field. By using external synchronization control equipment newly produced with the Nipkow confocal microscope, the system which could be observed for the first time real-time and three-dimensional was produced experimentally. Using this system, we have used observed that thrombocyte. morphological changes and Ca2+ propagation in myocardial cells.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] H.Ishida, N.Higashijima, Y.Hirota, C.Genka, H.Nakazawa, H.Nakaya, T.Sato: "Nicorandil attenuates the mitochondrial Ca^<2+> overload with accompanying depolarization of the mitochondrial membrane in heart."Naunyn Schmiedebergs Arch Pharmacol. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Z.Su, M.Sheets, H.Ishida, F.Li, W.H.Barry: "Saxitoxin Blocks L-type I_<Ca>."J Pharmacol Exp Ther. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 石田英之^1, 佐藤俊明^3, 東島直子^1, 広田有希^1, 源河朝広^2, 角野敏恵^4, 小泉 敬一^4, 星合 美奈子^4, 中谷晴昭^3, 中澤博江: "Nicorandilの心筋保護作用とPermeability Transition Poreの関係について"Therapeutic Research. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 石田英之: "細胞死におけるミトコンドリアPermeability Transition Pore(PTP)の役割に関する研究法"日本薬理学雑誌. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Ritter, Z.Su, K.D.Philipson, F.Li, K.W.Spitzer, H.Ishida, W.H.Barry: "Ca^<2+> Sparks Induced by Na/Ca Exchange"Cell Calcium. 34. 11-17 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Tanaami, S.Otsuki, N.Tomosada, Y.Kosugi, M.Shimizu, H.Ishida: "High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks"Applied Optics. 41. 4704-4708 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 石田英之, 広田有希, 東島直子, 小濱るりこ, 源河朝広, 中澤博江: "Nicorandilのミトコンドリア内Ca^<2+>過負荷抑制作用"Therapeutic Research. 23. 515-518 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida, Y.Hirota, C.Genka, H.Nakazawa, H.Nakaya, T.Sato: "Opening of mitochondrial K_<ATP> channels attenuates the ouabain-induced calcium overload in mitochondria."Circ Res. 89. 856-858 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 戸田英之, 広田有希, 源河朝広, 中澤博江, 田名網健雄: "ミトコンドリアATP感受性K^+チャンネル開口薬によるミトコンドリアCa^<2+>過負荷の改善"レーザー顕微鏡研究. 27. 13-17 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 石田英之, 広田有希, 源河朝広, 田名網健雄, 中澤博江: "超高速ニポウ式共焦点顕微鏡による心房筋細胞の細胞内Ca^<2+>制御の解明."心臓の機能と構造. 23. 17-21 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida, N.Higashijima, Y.Hirota, C.Genka, H.Nakazawa, H.Nakaya, T.Sato: "Nicorandil attenuates the mitochondrial Ca^<2+> overload with accompanying depolarization of the mitochondrial membrane in heart."Naunyn Schmiedebergs Arch Pharmacol. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Z.Su, M.Sheets, H.Ishida, F.Li, W.H.Barry: "Saxitoxin Blocks L-type I_<Ca>"J Pharmacol Exp Ther. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida, T.Sato, N.Higashijima, Y.Hirota, C.Genka, T.Kadono, K.Koizumi, M.Hoshiai, H.Nakaya, H.Nakazawa: "Modulation of PTP by Nicorandil."Therapeutic Research. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida: "The research method about the role of mitochondrial permeability transition pore in cell death."Jap J Pharmacol. (In press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Ritter, Z.Su, K.D.Philipson, F.Li, K.W.Spitzer, H.Ishida, W.H.Barry: "Ca^<2+> Sparks Induced by Na/Ca Exchange"Cell Calcium. 34. 11-17 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Tanaami, S.Otsuki, N.Tomosada, Y.Kosugi, M.Shimizu, H.Ishida: "High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks"Therapeutic Research. 23. 515-518 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida, Y.Hirota, N.Higashijima, R.Obama, C.Genka, H.Nakazawa: "Nicorandil attenuates the mitochondrial Ca^<2+> overload."Applied Optics. 41. 4704-4708 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida, Y.Hirota, C.Genka, H.Nakazawa, H.Nakaya, T.Sato: "Opening of mitochondrial K_<ATP> channels attenuates the ouabain-induced calcium overload in mitochondria."Circ Res. 89. 856-858 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida, Y.Hirota, C.Genka, H.Nakazawa, T.Tanaami: "Mitochondrial K_<ATP> channels opner attenuates the mitochondria calcium overload in rat cardiac myocytes."Society for Laser Microscopy. 27. 13-17 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida, Y.Hirota, C.Genka, T.Tanaami, H.Nakazawa: "A novel high-speed Nipkow cofocal laser scanning system captures intracellular Ca^<2+> transients in atrial cardiac myocytes."Structure and Metabolism of the Heart. 23. 17-21 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Ishida, N.Higashijima, Y.Hirota, C.Genka, H.Nakazawa, H.Nakaya, T.Sato: "Nicorandil attenuates the mitochondrial Ca^<2+> overload with accompanying depolarization of the mitochondrial membrane in the heart."Naunyn Schmiedebergs Arch Pharmacol. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Z.Su, M.Sheets, H.Ishida, F.Li, W.H.Barry: "Saxitoxin Blocks L-type I_<Ca>."J Pharmacol Exp Ther. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 石田英之, 佐藤俊明, 東島直子, 広田有希, 源河朝広, 角野敏恵, 小泉敬一, 星合美奈子, 中谷晴昭, 中澤博江: "Nicorandilの心筋保護作用とPermeability Transition Poreの関係について"Therapeutic Research. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 石田英之: "細胞死におけるミトコンドリアPermeability Transition Pore (PTP)の役割に関する研究法"日本薬理学雑誌. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Ritter, Z.Shu, K.D.Philipson, F.Li, K.W.Spitzer, H.Ishida, W.H.Barry: "Ca^<+2> sparks induced by Na/Ca exchange"Cell Calcium. (in press)(Abstract). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Tanaami, S.Otsuki, N.Tomosada, Y.Kosugi, M.Shimizu, H.Ishida: "High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks"Applied Optics. 41(22). 4704-4708 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Ishida1, Y.Hirota1, C.Genka1, H.Nakazawa1, N.Aosaki: "UNDERLYING MECHANISMS OF Ca^<2+> PROPAGATION DURING E-C COUPLING IN RAT ATRIAL MYOCYTES"Biophysical Journal. 82(in press/Abstract). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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