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Application of Copolymers of Lactic Acid and Amino Acid Having Reactive Side-Chain Groups as Biodegradable Materials for Tissue Engineering

Research Project

Project/Area Number 13558120
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionKansai University

Principal Investigator

OUCHI Tatsuro  Kansai University, Faculty of Engineering, Professor, 工学部, 教授 (60067650)

Co-Investigator(Kenkyū-buntansha) YAMADA Hideaki  Kuraray Co.Ltd., Kurashiki Labo., Researcher, 倉敷事業所・くらしき研究所(メディカル), 開発主管(研究職)
OHYA Yuichi  Kansai University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (10213886)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥10,400,000 (Direct Cost: ¥10,400,000)
KeywordsPoly(lactic acid) / Polydepsipeptide / Tissue Engineering / Regeneration / Biodegradable Materials / Cell culture / Copolymerization / Random copolymer / 細胞分化共重合 / 化学修飾
Research Abstract

In tissue engineering related to the regeneration of damaged or lost tissue, excellent biodegradable materials are desired as temporary scaffolds to support cell growth and disappear with the progress of tissue regeneration. We previously synthesized biodegradable poly(depsipeptide-random-lactide), poly[(Glc-Asp)-r-LA] and poly[(Glc-Lys)-r-LA] having reactive side-chain groups. In this study, to evaluate the utility of these copolymers as functional scaffolds for tissue regeneration, the effects of reactive and ionic side-chain groups on cell attachment and growth were investigated using copolymer films with various amounts of carboxyl or amino groups. Poly[(Glc-Lys)-r-LA] and poly[(Glc-Asp)-r-LA] films having appropriate positive or negative charges exhibited higher cell attachment ability than poly-L-lactide. Good cell growth was observed on the copolymer films. During cell culture, the copolymer films exhibited higher degradation rates related to the depsipeptide unit content. Biodegradable polymer matrices for cell growth, with reactive surfaces and controllable degradability, were successfully prepared using copolymers with various amounts of depsipeptide units. Moreover, to evaluate the utility of these copolyrners as functional scaffolds for tissue regeneration, three-dimensional porous sponges were prepared by freeze-drying method and the effects of reactive and ionic side-chain groups on cell growth and degradation behavior were investigated using copolymer sponges with various amounts of carboxyl or amino groups. Good cell growth was observed on the copolymer sponges. During cell culture, the copolymer sponges exhibited various degradation rates related to the depsipeptide unit content. Three-dimensional biodegradable polymer matrices with reactive surface, controllable degradation behavior and good cell growth were successfully prepared using these copolymers. Such kinds of copolymer matrices are good candidate for scaffold for tissue engineering.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] T.Ouchi, Y.Ohya(他4名): "Synthesis of Biodegradable Amphiphilic AB-Type Diblock Copolymers of Lactide and Depsipeptide with Pendant Reactive Groups"J.Polym.Sci.Part A Polym.Chem.. 40(9). 1218-1225 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, Y.Ohya(他4名): "Formation of Polymeric Micelle Having Amino Surface from Amphiphilic AB-Type Diblock Copolymers Composed of Poly(Glycolic Acid-Lysine) Segment and Polylactide Segment"J.Polym.Sci.Part A Polym.Chem.. 40(10). 1426-1432 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, Y.Ohya(他2名): "Preparation of Poly(L-Lactide)-Based Microspheres Having Cationic or Anionic Surface Using Biodegradable Surfactants"Biomacromolecules. 3(5). 885-888 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大矢裕一: "ポリ乳酸をベースとした新規な生分解性高分子の合成とバイオマテリアルとしての応用"高分子論文集. 59(8). 484-498 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大内辰郎, 大矢裕一: "新規なポリ乳酸系生分解性医用材料"未来材料. 2(3). 30-35 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ohya, H.Matsunami, T.Ouchi: "Cell Attachment and Growth on Films Prepared from Poly(depsipeptide-co-lactide) Having Various Functional Groups"J.Biomed.Mater.Res.. 65A(1). 79-88 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, Y.Ohya, H.Yamada, H.Arimura: "Preparation of Polylactide Microspheres Having Positively of Negatively Charged Surfaces"Macromol.Biosci.. 3(1). 18-25 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Arimura, Y.Ohya, T.Ouchi, H.Yamada: "Preparation of a biodegradable matrix through polyion complex formation by mixing polylactide-based microspheres having oppositely charged surfaces"J.Colloid Interface Sci.. 270(2). 299-303 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ohya, H.Matsunami, T.Ouchi: "Cell growth on the porous sponges prepared from poly(depsipeptide-co-lactide) having various functional groups"J.Biomat.Sci Polym.Edn.. 15(1). 111-124 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, Y.Ohya: "Desgin of Lactide Copolymers as Biomaterials"J.Polym.Sci.Part A Polym.Chem.. 42(3). 453-462 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, Y.Ohya(他2名): "Preparation of Poly[DL-lactide-co-glycolide]-Based Microspheres Containing Protein by Use of Amphiphilic Diblock Copolymers of Depsipeptide and Lactide Having Ionic Pendant Groups as Biodegradable Surfactant by W/O/W Emulsion Method"Polymer. 45(5). 1583-1589 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 大矢裕一: "多様な物性を示す新規なポリ乳酸系生分解性高分子の合成とバイオマテリアルとしての応用"バイオマテリアル. 22(1). 24-35 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, H.Miyazaki, H.Arimura, F.Tasaka, A.Hamada, Y.Ohya: "Synthesis of Biodegradable Amphiphilic AB-Type Diblock Copolymers of Lactide and Depsipeptide with Pendant Reactive Groups"J.Polym.Sci.Part A Polym.Chem.. 40(9). 1218-1225 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, H.Miyazaki, H.Arimura, F.Tasaka, A.Hamada, Y.Ohya: "Formation of Polymeric Micelle Having Amino Surface from Amphiphilic AB-Type Diblock Copolymers Composed of Poly(Glycolic Acid-Lysine) Segment and Polylactide Segment"J.Polym.Sci.Part A Polym.Chem.. 40(10). 1426-1432 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, M.Toyohara, H.Arimura, Y.Ohya: "Preparation of Poly(L-Lactide)-Based Microspheres Having Cationic or Anionic Surface Using Biodegradable Surfactants"Biomacromolecules. 3(5). 885-888 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ohya: "Synthesis of Novel Poly(Lactic Acid)-Based Biodegradable Polymers and Their Application as Biomaterials."Kobunshi Ronbunshu. 59(8). 484-498 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, Y.Ohya: "New Polylactic Acid Base Biodegradable Medical Materials."Mirai Zairyo. 2(3). 30-35 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ohya, H.Matsunami, T.Ouchi: "Cell Attachment and Growth on Films Prepared from Poly(Depsipeptide-co-Lactide) Having Various Functional Groups"J.Biomed.Mater.Res.. 65A(1). 79-88 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Arimura, Y.Ohya, T.Ouchi, H.Yamada: "Preparation of Polylactide Microspheres Having Positively or Negatively Charged Surfaces"Macromol.Biosci.. 3(1). 18-25 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Arimura, Y.Ohya, T.Ouchi, H.Yamada: "Preparation of a Biodegradable Matrix through Polyion Complex Formation by Mixing Polylactide-Based Microspheres Having Oppositely Charged Surfaces"J.Colloid Interface Sci.. 270(2). 299-303 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ohya, H.Matsunami, T.Ouchi: "Cell Growth on the Porous Sponges Prepared from Poly(depsipeptide-co-lactide) Having Various Functional Groups"J.Biomat.Sci Polym.Edn.. 15(1). 111-124 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, Y.Ohya: "Desgin of Lactide Copolymers as Biomaterials"J.Polym.Sci.Part A Polym.Chem.. 42(3). 453-462 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ouchi, M.Sasakawa, H.Arimura, Y.Ohya: "Preparation of Poly[DL-lactide-co-glycolide]-Based Microsheres Containing Protein by Use of Amphiphilic Diblock Copolymers of Depsipeptide and Lactide Having Ionic Pendant Groups as Biodegradable Surfactant by W/O/W Emulsion Method"Polymer. 45(5). 1583-1589 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Ohya: "Synthesis of Novel Biodegradable Lactic Acid Copolymers Exhibiting Various Properties and Their Application as Biomatenals"Journal of Japanese Society for Biomaterials. 22(1). 24-35 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Arimura, Y.Ohya, T.Ouchi, H.Yamada: "Preparation of a Biodegradable Matrix through Polyion Complex Formation by Mixing Polylactide-Based Microspheres Having Oppositely Charged Surfaces"J.Colloid Interface Sci.. 270(2). 299-303 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Ohya, H.Matsunami, T.Ouchi: "Cell Growth on the Porous Sponges Prepared from Poly(depsipeptide-co-lactide) Having Various Functional Groups"J.Biomat.Sci Polym.Edn.. 15(1). 111-124 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya: "Desgin of Lactide Copolymers as Biomaterials"J.Polym.Sci.Part A Polym.Chem.. 42(3). 453-462 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya(他2名): "Preparation of Poly[DL-lactide-co-glycolide]-Based Microsheres Containing Protein by Use of Amphiphilic Diblock Copolymers of Depsipeptide and Lactide Having Ionic Pendant Groups as Biodegradable Surfactant by W/O/W Emulsion Method"Polymer. 45(5). 1583-1589 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 大矢裕一: "多様な物性を示す新規なポリ乳酸系生分解性高分子の合成とバイオマテリアルとしての応用"バイオマテリアル. 22(1). 24-35 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya (他4名): "Synthesis of Biodegradable Amphiphilic AB-Type Diblock Copolymers of Lactide and Depsipeptide with Pendant Reactive Groups"J. Polym. Sci. Part A Polym. Chem.. 40(9). 1218-1225 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya (他4名): "Formation of Polymeric Micelle Having Amino Surface from Amphiphilic AB-Type Diblock Copolymers Composed of Poly(Glycolic Acid-Lysine) Segment and Polylactide Segment"J. Polym. Sci. Part A Plym. Chem.. 40(10). 1426-1432 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya (他2名): "Preparation of Poly(L-Lactide)-Based Microspheres Having Cationic or Anionic Surface Using Biodegradable Surfactants"Biomacromolecules. 3(5). 885-888 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大矢裕一: "ポリ乳酸をベースとした新規な生分解性高分子の合成とバイオマテリアルとしての応用"高分子論文集. 59(8). 484-498 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya, H.Yamada, H.Arimura: "Preparation of Polylactide Microspheres Having Positively or Negatively Charged Surfaces"Macromol. Biosci.. 3(1). 18-25 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Ohya, H.Matsunami, T.Ouchi: "Cell Attachment and Growth on Films Prepared from Poly(depsipeptide-co-lactide) Having Various Functional Groups"J. Biomed. Mater. Res.. 65A(1). 79-88 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Ohya(分担執筆): "Advances in Biomaterials and Drug Delivery Systems"Design of Novel Biodegradable Lactic Acid Copolymers for Biomaterials. 578 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] F.Tasaka, Y.Ohya, T.Ouchi: "Synthesis of Novel Comb-Type Polylactide and Its Biodegradablitiy"Macromolecules. 34・16. 5494-5500 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] F.Tasaka, Y.Ohya, T.Ouchi: "One-Pot Synthesis of Novel Branched Polylactide through Copolymerization of Lactide with Mevalonolactone"Macromol. Chem. Rapid Commun.. 22・11. 820-824 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ouchi, T.Uchida, Y.Ohya: "Synthesis of Poly(L-Lactic Acid) Having One Terminal D-Glucose Residue and Wettability of Its Film Surface"Macromol. Biosci.. 1. 371-375 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 大内辰郎, 大矢裕一: "生分解性バイオマテリアルの設計および医療用材料への応用"関西大学工業技術研究所報 技苑. 108. 34-35 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya (他4名): "Synthesis of Biodegradable Amphiphilic AB-Type Diblock copolymers of Lactide and Depsipeptide with Pendant Reactive Groups"J. Polym. Sci. Part A Polym. Chem.. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya (他4名): "Formation of Polymeric Micelle Having Amino Surface from Amphiphilic AB-Type Diblock Copolymers Composed of Poly(Glycolic Acid-Lysine) Segment and Polylactide Segment"J. Polym. Sci. Part A Polym. Chem.. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 大内辰郎, 大矢裕一: "新規なポリ乳酸系医用材料"未来材料. 2・3. 30-35 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Ohya, T.Ouchi(他2名)(分担執筆): "Biomedical Polymers and Polymer Therapeutics"Kluwer Academic/Plenum Publishers. 451 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ouchi, Y.Ohya(他3名)(分担執筆): "Chitin and Chitosan, Chitin and Chitosan in Life Science"Kodanshia Scientific Ltd.. 572 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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