| Project/Area Number |
13575029
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KARIWA Hiroaki Hokkaido Univ., Grad.School of Vet.Med., Assoc, Prof., 大学院・獣医学研究科, 助教授 (70224714)
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| Co-Investigator(Kenkyū-buntansha) |
ARIKAWA Jiro Hokkaido Univ., Grad.School of Med, Prof., 大学院・医学研究科, 教授 (10142704)
MIZUTANI Tetsuya National Institute of Infectious Diseases., Chief Researcher, 主任研究員 (70281681)
TAKASIMA Ikuo Hokkaido Univ., Grad.School of Vet.Med., Prof., 大学院・獣医学研究科, 教授 (30002083)
IWASAKI Takuya Nagasaki Univ., Institute of Tropical Med., Prof., 熱帯医学研究所, 教授 (90146027)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | zoonosis / rodent / epidemiology / hemorrhagic fever with renal syndrome / tick-borne encephalitis |
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| Research Abstract |
To know the epidemiology of viral zoonotic diseases such as hemorrhagic fever with renal syndrome (HFRS) and tick-borne encephalitis (TBE) in Far East Russia, we Conducted an epizootiological survey in Khabarovsk. Anti-hantavirus antibodies were detected in Apodemus.agrarius (5/47), and Clethrionomys rufocanus (7/40) among 102 small animals captured. Partial M segments were amplified from seropositive A.agrarius. The virus nucleotide sequence in G2 region (2695-2926nt) from A agrarius had 97.0 -99.6% identities with those of FE genotypes which were identified from blood samples of HFRS patients. On the contrary, the sequence had 80.7 to 82.5% identities with those of AMR. These results suggest that hantavirus FE genotype cases severe HFRS and is carried by A agrarius. We established the ELISA to detect anti-TBE virus antibodies by using the recombinant viral E protein expressed in E.coli expression system. The ELISA could detected anti-TBE virus antibodies in C.rufocanus captured in Khabarovsk, which were also positive by authentic neutralization test. This indicate that the ELISA can be a useful method to identify the endemic areas of TBE.
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