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Effect of iron as an environmental poisonous compound upon the precocious development of human hepatocellular carcinoma in Myanmar

Research Project

Project/Area Number 13576001
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagasaki University

Principal Investigator

KOJI Takehiko  Nagasaki University, Graduate School of Biomedical Sciences, Department of Developmental and Reconstructive Medicine, Professor, 大学院・医歯薬学総合研究科, 教授 (30170179)

Co-Investigator(Kenkyū-buntansha) IZUMI Shinichi  Nagasaki University, Graduate School of Biomedical Sciences, Department of Developmental and Reconstructive Medicine, Instructor, 大学院・医歯薬学総合研究科, 助手 (40264246)
EJIMA Kuniaki  Nagasaki University, Graduate School of Biomedical Sciences, Department of Developmental and Reconstructive Medicine, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (30309984)
HISAKAWA Yoshitaka  Nagasaki University, Graduate School of Biomedical Sciences, Department of Developmental and Reconstructive Medicine, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (60304276)
ABE Kuniko  Nagasaki University Hospital of Medicine and Dentistry, Instructor, 医学部・歯学部附属病院, 助手 (00253641)
OKADA Shigeru  Okayama University, Graduate School of Medicine and Dentistry, Department of Pathological Research, Professor, 大学院・医歯学総合研究科, 教授 (20033201)
進 正志  長崎大学, 大学院・医歯薬学総合研究科, 助手 (80145226)
石川 隆俊  大学評価・学位授与機, 構, 教授 (30085633)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2003: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥6,100,000 (Direct Cost: ¥6,100,000)
KeywordsMyanmar / hepatocellular carcinoma / iron / iron-responsive element binding proteins / hepatitis viruses / apoptosis / Fas / Fas ligand / molecular histochemistry / Fasリガンド / 肝炎ウィルス
Research Abstract

The precocious development of hepatocellular carcinoma (HCC) is a crucial problem in Myanmar. As a possible cause, iron incorporated from the environment is implicated. In the present study, we investigated the deposition of iron and the expression of iron-responsive element binding protein (IREBP) in the tissue sections from Myanmar HCC, and then the correlation between these iron-related parameters and HCC cell kinetics under the intimate cooperation with Department of Medical Research (DMR). Actually, we collected cancerous and non-cancerous parts of more than 30 HCC patients at our 3-times visit to Yangon (December 18 to 24, 2001, December 19-28, 2002 and December 18-23, 2003). During our visit, we also' held not only several meetings with DMR and Medical University scientists and but workshops on TUNEL in 2002 and nonradioactive in situ hybridization in 2003 in DMR, which successfully promoted our friendships. When Myanmar HCC tissue sections were examined for iron deposition, we found significantly heavier deposition of iron in Myanmar HCC than that of Japanese one. However, we failed to analyze the expression of IREBP effectively because of the inevitable loss of structural details after microwave retrieval of reactivity. In the study on the HCC cell kinetics, the specimens with a high proliferating activity assessed by Ki-67 staining was correlated with a low frequency of TUNEL positive cells while low proliferative cases were with frequent apoptosis. In the latter case, an apparent association with Fas ligand expression was found. Moreover, when we investigated the liver regeneration kinetics after partial hepatectomy in iron-overloaded rat model, a significant precocious starting of regeneration was observed, accompanying overall increase in the rate of apoptosis. These results indicate that acceleration of hepatocyte turnover by overloaded iron may promote the development of HCC under the proliferation-induced conditions such as viral infection.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (54 results)

All Other

All Publications (54 results)

  • [Publications] Fukuzawa, K.: "Expression of Fas/Fas ligand (FasL) and its involvement in infiltrating lymphocytes in hepatocellular carcinoma (HCC)."J.Gastroenterol.. 36・10. 681-688 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hishikawa, Y.: "Expression of metallothionein in colorectal cancers and synchronous liver metastases."Oncology. 61・2. 162-167 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taira, K.: "Role of the Fas system in liver regeneration after a partial hepatectomy in rats."Eur.Surg.Res.. 33・5-6. 334-341 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sawada, T.: "Partial tolerance of subcutaneously transplanted xenogeneic tumour cell graft by Fas-mediated immunosuppression."Immunology. 103・1. 81-89 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goto, S.: "Doxorubicin-induced DNA intercalation and scaveniging by nuclear glutathione S-transferase π."FASEB J.. 15・14. 2702-2714 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koji, T.: "Expression of Fas and Fas ligand in normal and ischemia-reperfusion testes : Involvement of the Fas system in the induction of germ cell apoptosis in the damaged mouse testis."Biol.Reprod.. 64・3. 946-954 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koji, T.: "Male germ cell death in mouse testes : Possible involvement of Fas and Fas ligand(Review)."Medical Electron Microsc.. 34・4. 213-222 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shukuwa, T.: "Fas-mediated apoptosis of melanoma cells and infiltrating lymphocytes in human malignant melanomas."Modern Pathol.. 15・4. 387-396 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shibata, Y.: "Involvement of Fas/Fas ligand in the induction of apoptosis in chronic sialadenitis of minor salivary glands including Sjogren's syndrome."Hum.Cell. 15・1. 52-60 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin, M.: "Southwestern histochemistry as a molecular histochemical tool for analysis of expression of transcription factors : Application to paraffin-embedded tissue sections."Med.Electron Microsc.. 35・4. 217-224 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kondo, T: "Diethylstilbestrol attenuates antioxidant activities in testis from male mice."Free Radic.Res.. 36・9. 957-966 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hishikawa, Y.: "Molecular histochemical analysis of estrogen receptor α and β expressions in the mouse ovary : In situ hybridization and Southwestern histochemistry."Med.Electron Microsc.. 36・2. 67-73 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Imaizumi, Y.: "Expression of the c-Met proto-oncogene and its possible involvement in liver invasion in adult T-cell leukemia."Clin.Cancer Res.. 9・1. 181-187 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ashizawa, M.: "Detection of nuclear facator-kB in IgA nephropathy using southwestern histochemistry."Am.J.Kidney Dis.. 42・1. 76-86 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小路武彦: "サウスウェスタン組織化学-特異的転写因子の視覚的局在化法"医学のあゆみ. 199・13. 850-855 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小路武彦: "分子組織細胞化学:遺伝子発現をみる"日本臨床細胞学会九州連合会雑誌. 33. 1-8 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 菱川善隆: "In situ hybridizationによるゲノム転写産物の局在証明と定量的解析"細胞. 35・12. 489-493 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小路武彦: "医学大辞典(分担執筆)"医学書院. (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小路武彦: "In situ hybridizationと免疫組織化学[改訂四版 渡辺・中根 酵素抗体法]"学際企画(名倉宏, 長村義之, 堤寛編). 8 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小路武彦: "分子組織細胞化学:mRNAの検出から転写因子の解析まで[組織細胞化学2002](日本組織細胞化学会編)"学際企画. 11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小路武彦: "遺伝子組織化学総論[組織細胞化学2002](日本組織細胞化学会編)"学際企画. 12 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuzawa, K., Takahashi, K., Furuta, K., Tagaya, T., Ishikawa, T., Wada, K., Omoto, Y., Koji, T., Kakumu, S: "Expression of Fas/Fas ligand (FasL) and its involvement in infiltrating lymphocytes in hepatocellular carcinoma (HCC)."J.Gastroenterol.. 36(10). 681-688 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hishikawa, Y., Kohno, H., Ueda, S., Kimoto, T., Kumar Dhar, D., Kubota, H., Tachibana, M., Koji, T., Nagasue, N.: "Expression of metallothionein in colorectal cancers and synchronous liver metastases."Oncology. 61(2). 162-167 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taira, K., Hiroyasu, S., Shiraishi, M., Muto, Y., Koji, T.: "Role of the Fas system in liver regeneration after a partial hepatectomy in rats."Eur.Surg.Res.. 33(5-6). 334-341 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sawada, T., Koji, T., Hishikawa, Y., Kishimoto, K., Nagayasu, T., Takahashi, T., Oka, T., Ayabe, H.: "Partial tolerance of subcutaneously transplanted xenogeneic tumour cell graft by Fas-mediated immunosuppression."Immunology. 103(1). 81-89 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Goto, S., Ihara, Y., Urata, Y., Izumi, S., Abe, K., Koji, T., Kondo, T: "Doxorubicin-induced DNA intercalation and scaveniging by nuclear glutathione S-transferase π."FASEB J.. 15(14). 2702-2714 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koji, T., Hishikawa, Y., Ando, H., Nakanishi, Y., Kobayashi, N.: "Expression of Fas and Fas ligand in normal and ischemia-reperfusion testes : Involvement of the Fas system in the induction of germ cell apoptosis in the damaged mouse testis."Biol.Reprod.. 64(3). 946-954 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koji, T.: "Male germ cell death in mouse testes : Possible involvement of Fas and Fas ligand (Review)"Medical Electron Microsc.. 34(4). 213-222 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shukuwa, T., Katayama, I., Koji, T.: "Fas-mediated apoptosis of melanoma cells and infiltrating lymphocytes in human malignant melanomas."Modern Pathol.. 15(4). 387-396 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shibata, Y., Hishikawa, Y., Izumi, S., Fujita, S., Yamaguchi, A., Koji, T.: "Ivolvement of Fas/Fas ligand in the induction of apoptosis in chronic sialadenitis of minor salivary glands including Sjogren's syndrome."Hum.Cell. 15(1). 52-60 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin, M., Hishikawa, Y., Izumi, S., Koji, T.: "Southwestern histochemistry as a molecular histochemical tool for analysis of expression of transcription factors : Application to paraffin-embedded tissue sections."Med.Electron Microsc.. 35(4). 217-224 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kondo, T., Goto, S., Ihara, Y, Urata, Y., Ikeda, S., Hishikawa, Y., Izumi, S., Shin, M.Koji, T.: "Diethylstilbestrol attenuates antioxidant activities in testis from male mice."Free Radic.Res.. 36(9). 957-966 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hishikawa, Y., Damavandi, E.Izumi, S., Koji, T.: "Molecular histochemical analysis of estrogen receptor rx and /3 expressions in the mouse ovary : In situ hybridization and Southwestern histochemistry."Med.Electron Microsc. (and Cover). 36(2). 67-73 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Imaizumi, Y., Murota, H., Kanda, S., Hishikawa, Y., Koji, T., Taguchi, T., Tanaka, Y, Yamada, Y., Ikeda, S., Kohno, T., Yamamoto, K., Mori, N., Tomonaga, M., Matsuyama, T.: "Expression of the c-Met proto-oncogene and its possible involvement in liver invasion in adult T-cell leukemia."Clin.Cancer Res.. 9(1). 181-187 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ashizawa, M., Miyazaki, M., Abe, Katsushige, Furusu, A., Isomoto, H., Harada, T., Ozono, Y., Sakai, H., Koji, T., Kohno, S.: "Detection of nuclear facator-kB in IgA nephropathy using southwestern histochemistry."Am.J.Kidney Dis.. 42(1). 76-86 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Imaizumi, Y.: "Expression of the c-Met proto-oncogene and its possible involvement in liver invasion in adult T-cell leukemia"Clin.Cancer Res.. 9・1. 181-187 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okada, K.: "Expression of tumor-associated membrane antigen, RCAS1, in human colorectal carcinomas and possible role in apoptosis of tumor-infiltrating lymphocytes"Modern Pathol.. 16・7. 679-685 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Koji, T.: "Germ cell apoptosis and its molecular trigger in mouse testes (Review)"Arch.Histol.Cytol.. 66・1. 1-16 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto-Fukuda, T.: "Possible involvement of keratinocyte growth factor and its receptor in enhanced epithelial-cell proliferation and acquired recurrence of middle-ear cholesteatoma"Lab.Invest.. 83・1. 123-136 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hishikawa, Y.: "Molecular histochemical analysis of estrogen receptor α and β expressions in the mouse ovary : In situ hybridization and Southwestern histochemistry"Med.Electron Microsc.. 36・2. 67-73 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ashizawa, M.: "Detection of nuclear facator-kB in IgA nephropathy using southwestern histochemistry"Am.J.Kidney Dis.. 42・1. 76-86 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 小路武彦: "遺伝子組織化学総論[組織細胞化学2003](日本組織細胞化学会編)"学際企画. 12 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shukuwa, T.: "Fas-mediated apoptosis of melanoma cells and infiltrating lymphocytes in human malignant melanomas"Modern Pathol.. 15・4. 387-396 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujita, H.: "Possibile involvement of Fas system in the induction of apoptosis in human astrocytic brain tumors"Cell Mol. Neurobiol. 22・4. 393-406 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Isomoto, H.: "Enhanced expression of transcription factor E2F in Helicobacter Pylori-infected gastric mucosa"Helicobacter. 7・3. 152-162 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shin, M.: "Southwestern histochemistry as a molecular histochemical tool for analysis of expression of transcription factors : Application to paraffin-embedded tissue sections"Med. Electron Microsc.. 35・4. 217-224 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sun, F.: "Apoptosis in normal rat embryo tissues during early organogenesis : The possible involvement of Bax and Bcl-2"Arch. Histol. Cytol. 65・2. 145-157 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kondo, T.: "Diethylstilbestrol attenuates antioxidant activities in testis from male mice"Free Radic. Res.. 36・9. 957-966 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukuzawa, K.: "Expression of Fas/Fas ligand (FasL) and its involvement in infiltrating lymphocytes in hepatocellular carcinoma (HCC)"J.Gastroenterol. 36・10. 681-688 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hishikawa, Y.: "Expression of metallothionein in colorectal cancers and synchronous liver metastases"Oncology. 61・2. 162-167 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Goto, S.: "Doxorubicin-induced DNA intercalation and scaveniging by nuclear glutathione S-transferase π"FASEB J. 15・Dec. 2702-2714 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sawada, T.: "Partial tolerance of subcutaneously transplanted xenogeneic tumour cell graft by Fas -mediated immunosuppression"Immunology. 103・1. 81-89 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abe, K.: "Enhanced expression of complement C5a receptor mRNA in human diseased kidney assessed by situ hybridization"Kidney Int. 60・1. 137-146 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Koji, T.: "Expression of Fas and Fas ligand in normal and ischemia -reperfusion tests : Involvement of the Fas system in the induction of germ cell apoptosis in the damage mouse test"Biol.Reprod.. 64・3. 946-954 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2021-09-24  

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