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The study of the initiation of the flowering using Arabidopsis mutants.

Research Project

Project/Area Number 13640613
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionThe University of Tokyo (2002)
Hokkaido University (2001)

Principal Investigator

KOMEDA Yoshibumi  The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (10124215)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsArabidopsis thaliana / flowering / mutants / initiation of flowering / シロイヌナマズ / 花芽 / 暗黒下 / ショ糖 / cop1-6 / 暗黒花芽形成 / もやし / AP1遺伝子 / 花成遅延遺伝子 / gi変異遺伝子 / cop1-6変異体 / 花芽形成関連遺伝子
Research Abstract

The initial finding is that the cop1-6 mutant can develop to make flowers in complete darkness. It was initial interest that the expression or not of the genes whose functions are responsible to complete the process of the initiation of the floral development. We detected the expression of AP1, CAL, and AG genes in dark grown cop1-6 mutant by RT-PCR method, which can detect the minor amount of messages.
The analysis using the double mutants of cop1-6 with co, gi and ld mutations revealed the following results : the functional point of COP1 is after that of CRY2 gene and before those of C0, GI, and LD.
We also observered the effect of the sugars in the growth medium of dark culture. The results were as follows : cop1-1, cop1-4, and cop1-6 mutants could develop flowers from the concentrations of sucrose at 8%, 5% and 2%, respectively. Thus, the dark flowering may be called as induction of flowering by sucrose instead of light.
We finally determined the location of the cop1-6 mutated product by the fusion of the cop1-6 mutation with the GFP=jelly fish green florescent protein. We found the location of the cop1-6 pruduct in nucleus and cytoplasm, and thus explained the phenotype of the cop1-6 mutation by this defect.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 半澤, 今井, Michal, 米田, 高橋: "Characterization of the spermidine synthase-related gene family in Arabidopsis thaliana"FEBS Letters. 527. 176-180 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 高橋, 松原, 阿部, 米田: "Disruption of a DNA topoisomerase I gene affects morphogenesis in Arabidopsis"The Plant Cell. 14(9). 2085-2093 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 鈴木, 加藤, 永田, 米田: "Axylanase, AtXyn1, is predominantly expressed in vascular bundles, and four putative"Plant and Cell Physiology. 43(7). 759-767 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 鈴木, 高橋, 米田: "Formation of corymb-like inflorescences due to delay in bolting and flower development in the corymbosa2 mutant of Arabidopsis"Plant and Cell Physiology. 43(3). 298-306 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 木村, 永里, 米田, 奥田: "Transient localization of γ-tubulin around the centrioles in the nuclear division of Boergesenia forbesii (Siphonocladales, chlorophyta)"J. Phycology. 37. 783-792 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 阿部, 高橋, 米田: "Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein"The Plant Journall. 26(5). 487-494 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 倉田, 中川, 米田, 荒木: "花・性と生植の分子生物学(pp25-40)"学会出版センター. 258 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Abe, Y. Komeda, and Takahashi, T.: "Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis"Development. 130. 635-643 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Hanzawa, A. Imai, A. J. Michael, Y. Komeda, and T. Takahashi: "Characterization of the spermidine synthase-related gene family in Arabidopssis thaliana"FEBS Letters. 527. 176-180 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Taku Takahashi, Shio Matsuhara, Mitsutomo Abe, and Yoshibumi Komeda: "Disruption of a DNA topoisomerase I gene affects morphogenesis in Arabidopsis"Plant Cell. 14(9). 2085-2093 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Suzuki, A. Kato, N. Nagata, and Y. Komeda: "A xylanase, AtXynl, is predominantly expressed in vascular bundles, and four putative xylanase genes were identified in the Arabidopsis thaliana genome"Plant and Cell Physiology. 43(7). 759-767 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Suzuki, T. Takahashi and Y. Komeda: "Formation of corymb-like inflorescences due to delay in bolting and flower development in the corymbosa2 mutant of Arabidopsis"Plant and Cell Physiology. 43(3). 298-306 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Motomura, C. Nagasato, Y. Komeda and K. Okuda: "Transient localization of g-tubulin around the centrioles in the nuclear division of Boergesenia forbesii (siphonocladales, chlorophyta)"J. Phycol.. 37. 783-792 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Abe, T. Takahashi and Y. Komeda: "Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein"The Plant Journal. 26(5). 487-494 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Suzuki, T.Takahashi, Y.Komeda: "Formation of corymb-like inflorescences due to delay in bolting and flower development in the corymbosa2"Plant and Cell Physiology. 43(3). 298-306 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Suzuki, A.Kato, N.Nagata, Y.Komeda: "A xylanase, AtXyn1, is predominantly expressed in vascular bundles, and flour putative putatine xylanase genes"Plant and Cell Physiology. 43(7). 759-767 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe, M.: "Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein"The Plant Journal. 26(5). 487-494 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki, Mituhiro: "Molecular genetic analysis of CRM2 ; an Arabidopsis mutant with corymb-like inflorescence"Plant and Cell Physiology. 43. 52 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hanazawa, Yoshie: "Characterization of the spermidine synthase gene family in Arabidopsis thaliana"Plant and Cell Physiology. 43. 91 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abe, M.: "PROTODERMAL FACTOR2, a gene encoding a homeodomain protein, regulates epidermal cell differentiation in the Arabidopsis shoot"The Plant Journal. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 倉田哲也: "花芽と花序の分化 花・性と生殖の分子生物学"(学会出版センター)編著 : 日向康吉 編集委員 : 磯貝 彰/鎌田 博/米田 好文/長戸 康郎/鳥山 欽哉. 16 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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