• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Meristem construction and control of CDK activities in plants

Research Project

Project/Area Number 13640642
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionThe University of Tokyo

Principal Investigator

UEDA Masaaki  The University of Tokyo, Institute of Molecular and Cellular Biosciences, Associate Professor, 分子細胞生物学研究所, 助教授 (80221810)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsPlant / Cell division / Cell cycle / Meristem / Cyclin-dependent kinase / Cyclin / CDK活性化キナーゼ / RNAポリメラーゼII / 分化 / リン酸化
Research Abstract

In this project, we aimed to reveal the regulatory :mechanisms underlying CDK activities that control cell proliferation in plant. meristems. For the full activation of CDKs, not only cyclin binding but also phosphorylation is required. This phosphorylation is catalyzed by CDK-activating kinase (CAK). In Arabidopsis, there exist four CAKs, CAKI to CAK4. Among them, CAK2 and CAK4 can be classified into the vertebrate-type CAK in terms of enzyme activity, whereas each shows different substrate specificies. Namely, CAK2 and CAK4 preferentially phosphorylated CDK and the carboxy-terminal domain (TD) of the largest subunit of RNA polymerase II, respectively. Moreover, we found that CAK 1 phosphorylates CAK2 and CAK4, and activate the CTD-kinase activity of CAK4. These results suggested that Arabidopsis has a CDK-and CTD-phosphorylation cascade mediated by three CAKs.
We overexpressed the cDNA of a B2-type cyclin, CycB2;2, in rice plants by using a glucocorticoid induction system. Our analyses revealed that the overexpression resulted in acceleration of root growth without any change in cell elongation. This indicated that cell proliferation in the root meristem had been activated by CYCB2;2 overexpression. By in vitro pull-down experiments, we showed that CYCB2;2 specifically interacted with CDKB2, and this interaction enhanced the kinase activity of CDKB2. Based on these data, we concluded that CYCB2;2 overexpression up-regulated the CDKB2 activity, which in turn promoted the transition from G2-to-M phase to accelerate the cell cycle duration in the root meristem.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Fabian-Marwedel, T.: "The rice cyclin-dependent kinase-activating kinase R2 regulates S-phase progression."Plant Cell. 14. 197-210 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bako, L.: "The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants."Proc.Natl.Acad.Sci.,USA. 100. 10108-10113 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, M.: "Control of in vitro organogenesis by cyclin-dependent kinase activities in plants."Proc.Natl.Acad.Sci.,USA. 100. 8019-8023 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kono, A.: "Arabidopsis D-type cyclin CYCD4;1 is a novel cyclin partner of B2-type cyclin-dependent kinase."Plant Physiol.. 132. 1315-1321 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Lee, J.: "Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase."Plant J.. 34. 417-425 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimotohno, A.: "Differential phosphorylation activities of CDK-activating kinases in Arabidopsis thaliana."FEBS Letter. 534. 69-74 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fabian-Marwedel, T., Umeda, M., Sauter, M.: "The rice cyclin-dependent kinase-activating kinase R2 regulates S-phase progression."Plant Cell. 14. 197-210 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Bako, L., Umeda, M., Tiburcio, A.F., Schell J., Koncz, C: "The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants."Proc.Natl.Acad.Sci., USA. 100. 10108-10113 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, M., Kato, H., Yoshida, S., Yamamura, S., Uchimiya, H., Umeda, M.: "Control of in vitro organogenesis by cyclin-dependent kinase activities in plants."Proc.Natl.Acad.Sci., USA. 100. 8019-8023 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kono, A., Umeda-tiara, C., Lee, J., Ito, M., Uchimiya, H., Umeda, M.: "Arabidopsis D-type cyclin CYCD4,1 is a novel cyclin partner of B2-type cyclin-dependent kinase."Plant Physiol. 132. 1315-1321 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Lee, J., Das, A., Yamaguchi, M., Hashimoto, J., Tsutsumi, N., Uchimiya, H., Umeda, M.: "Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase."Plant J. 34. 417-425 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shimotohno, A., Matsubayashi, S., Yamaguchi, M., Uchimiya, H., Umeda, M.: "Differential phosphorylation activities of CDK-activating kinases in Arabidopsis thaliana."FEBS Lett. 534. 69-74 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamaguchi, M.: "Control of in vitro organogenesis by cyclin-dependent kinase activities in plants."Proc.Natl.Acad.Sci., USA. 100. 8019-8023 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Bako, L.: "The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants."Proc.Natl.Acad.Sci., USA. 100. 10108-10113 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Barrero, R.A.: "Over-expression of Arabidopsis CAP causes decreased cell expansion leading to organ size reduction in transgenic tobacco plants."Ann.Bot. 91. 599-603 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Barrero, R.A.: "Arabidopsis CAP regulates actin cytoskeleton necessary for the plant cell elongation and division"Plant Cell. 14. 149-163 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fabian-Marwedel, T.: "The rice cyclin-dependent kinase-activating kinase R2 regulates S-phase progression"Plant Cell. 14. 197-210 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yanagawa, Y.: "Spatial distribution of the 26S proteasome in meristematic tissues and primordia of rice (Oryza sativa L.)"Planta. 214. 703-707 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimotohno, A.: "Differential phosphorylation activities of CDK-activating kinases in Arabidopsis thaliana"FEBS Letter. 534. 69-74 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Umeda, M.: "Cdk-Activating Kinase (CAK)"Landes Bioscience, Georgetown, Texas. 10 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Pan, L.: "The Arabidopsis thalilana ethylene-responsive element binding protein (AtEBP) can function as a dominant suppressor of Bax-induced cell death of yeast"FEBS Letter. 508. 375-378 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Barrero, R.A.: "Arabidopsis CAP regutates actin cytoskeleton necessary for the plant cellelongation and division"Plant Cell. 14. 149-163 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fabian-Marwedel, T.: "The rice cyclin-dependent kinase-activating kinase R2 regulates S-phase progression"Plant Cell. 14. 197-210 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Umeda, M.: "The CDK-Activating Kinase (CAK)"Landes Bioscience, Texas. 10 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uchimiya, H.: "Rice Genetics IV"International Rice Research Institute, Los Banos (Philippines). 12 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi