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Anovel degradation pathway of the D1 protein under light stress

Research Project

Project/Area Number 13640650
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

YAMAMOTO Yasusi  Okayama University, Graduate School of Natural Science and Technology, Professor, 大学院・自然科学研究科, 教授 (40091251)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
KeywordsPhotosvstem II / Photoinhibition / Reactive oxygen species / Protease / Protein aggregation / Oxygen evolution / Reaction center / D1 protein / 光合成 / D1タンパク質 / 品質管理 / タンパク質架橋
Research Abstract

Under light stress conditions, the reaction center binding D1 protein of photosystem II is degraded rapidly. In the present study, we found a novel pathway of D1 degradation. We characterized the pathway in detail and also studied the components involved in the pathway. The main results obtained are as follows :
1. Isolation of a protease(s) degrading the cross-linked products of the D1 protein from the stioma : During the photoinhibition of photosystem II, the D1 protein cross-links with the surrounding polypeptides. We partiafly purified a protease(s) that recognizes and digests the cross-linked products from the fraction of stroma, using a gelatin activity gel.
2. Determination of spatial arrangement of the D1 protein and neighboring polypeptides in photosystem II with the use of phofco-crossnnking reaction : We determined the relative arrangement of the D1 protein, CP43 andOEC33.in the lumenal side of photosystem II. These proteins are essential for the oxygen evolving activity of photosystem II, and the information obtained here is crucial for the structural analysis of the oxygen evolving system.
3. Induction of singlet oxygen under heat and light stresses in photosystem II and the generation of cross-linked products of the D1 protein with the neighboring polypeptides : We found that formation of singlet oxygen by the illumination of PS II is stimulated under heat stress. The singlet oxygen was the main cause for the protein cross-linking in photosystem II.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] A.Ferjani: "Characterization of the stromal protease(s) degrading the cross-linked products of the D1 protein generated by photoinhibition of photosystem II"Biochim.Biophys. Acta. 1503. 385-395 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Yamamoto: "Quality control of photosystem II"Plant Cell Physiol.. 42. 121-128 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Ishikawa: "Chemical modification of amine groups on PS II protein(s) retards photoassembly of the photosynthetic water-oxidizing complex"Biochemistry. 41. 1972-1980 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Takahashi: "High-susceptibility of photosynthesis to photoinhibition in the tropical plant Ficus microcarpa L.f.cv.Golden Leaves"BMC Plant Biology. 2:2. 1-24 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Tohri: "Comparison of the structure of the extrinsic 33 kDa protein from different organisms"Plant Cell Physiol.. 43. 429-439 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Henmi: "Dynamic interaction between the Dl protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts : evidence from light induced cross-linking of the proteins in the donor-side photoinhibition"Plant Cell Physiol.. 44. 451-456 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Yamamoto: "Photosynthesis Protocols (Ed. by R.Carpentier)"Humana Press(U.S.A.). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A Ferjani, et at: "Characterization of the stromal proteasef(s) degrading the cross-linked products of the D1 protein generated by photoinhibition of photosystem II."Biochim. Biophys. Acta.. 1503. 385-395 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Yamamoto: "Quality control of photosystem II."Plant Cell Physiol. 42. 121-128 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Ishikawa, et al.: "Chemical modification of amine groups on PS II protein(s) retards photoassembly of the photosynthetic water oxidizing complex."Biochemistry. 41. 1972-1980 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Takahashi, et al.: "High-susceptibility of photosynthesis to photoinhibition in the tropical plant Ficus microcarpa L. f. cv. Golden Leaves."BMC Plant Biology. 2:2. 1-24 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Tohri, et al.: "Comparison of the structure of the extrinsic 33 kDa protein from different organisms."Plant Cell Physiol. 43. 429-439 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Henmi, et al.: "Dynamic interaction between the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chtoroplasts : evidence from light induced cross-linking of the proteins in the donor-side photoinhibition."Plant Cell Physiol.. 44. 451-456 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Takahashi: "High-susceptibility of photosynthesis to photoinhibition in the tropical plant Ficus microcarpa L.f.cv.Golden Leaves"BMC Plant Biology. 2:2. 1-24 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Henmi: "Dynamic interaction between the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts"Plant and Cell Physiology. 44(4)(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Yamamoto: "Photosynthesis Protocols (Ed. by R. Carpentier)"Humana Press (U.S.A). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Ishikawa: "Chemical modification of the amino groups on the PS2 protein(s) retards photoassembly of the photosynthetic water-oxidizing complex"Biochemistry. 41. 1972-1980 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Tohri: "Comparison of the structure of the extrinsic 33kDa protein from different organisms"Plant and Cell Physiology. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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