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Gene regulations of fish gonadotropin receptor, unity and diversity

Research Project

Project/Area Number 13640671
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionTeikyo University of Science and Technology

Principal Investigator

HIRAI Toshiaki  Teikyo University of Science and Technology, Department of Biosciences, Assistant professor, 理工学部, 助手 (30238331)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsgonadotropin / receptor / gene / GTH / spermatogenesis / oogenesis / glycoprotein hormone
Research Abstract

As a first step to elucidate the unity and diversity of fish gonadotropin receptor (GTH-R), comparative study for molecular structure and gene expression profiles were performed. Initially, we established a degenerate RT-PCR system on the basis of the structures of known mammalian glycoprotein hormone receptor family including GTH-Rs. Although we succeeded to clone cDNAs from several species using this system, it often gave nonspecific products and required further complicated process to obtain receptor cDNAs. Therefore, we newly designed two primer sets on the basis of conserved sequences of known fish GTH-Rs. The new primer sets successfully amplified cDNA fragments of 187bp (between ECDI and TMD2) and 257bp (between ECD3 and TMD6) from all species attempted. To date, we have succeeded to clone both types of GTH-R (FSH-R and LH-R) cDNAs from 11 species belonging 7 different orders (including results of some collaborations). These results suggest that this PCR cloning system has high applicability to wide range of fish species. Change of mRNA levels for the two types of receptor during gametogenesis were examined in several species. In amago salmon oogenesis, FSH-R mRNA was high at early vitellogenic stage and decreased toward the maturational stage, whereas LH-R mRNA showed a peak at the final maturation. A similar profile was observed in the process of oocyte development of the leading clutch of tilapia. In medaka ovary, both types of receptor mRNAs were detected at vitellogenic stage, while FSH-R mRNA was disappeared earlier toward the maturational stage. In male of salmon and sculpin, both types showed similar pattern with a peak at spermiation. Finally, 5'-flanking regions of genes for FSH-R (1.4kb) and LH-R (2.2kb) were cloned from amago salmon to characterize their regulatory elements. Preliminary reporter assay showed that neither estrogen nor androgen do not directly affect their promoter activities.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Oba, Y.et al.: "Fish gonadotropin and thyrotropin receptors : the evolution of glycoprotein hormone receptors in vertebrates"Comparative Biochemistry and Physiology B. 129. 441-448 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mita, M. et al.: "Glycoprotein hormone-receptor family in ovaries of starfish, Asterina pectinifera"14th International Congress of Comparative Endocrinology. 1147-1152 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirai, T. et al.: "Fish gonadotropin receptors : molecular characterization and expression during gametogenesis"Fisheries Science. 68・Sup.1. 675-678 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mita, M. et al.: "cDNA cloning and functional analysis of a novel member of the glycoprotein hormone receptor family from a starfish Asterina pectinifera"Molecular Reproduction & Development. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirai, T. et al.: "Glycoprotein hormone receptor family in medaka (Oryzias latipes)"Fish Physiology and Biochemistry. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Rahman, K. et al.: "Gonadotropins, Gonadotropin receptors and their expressions during sexual maturation in yellowtail, a carangid fish"Fish Physiology and Biochemistry. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Oba, Y. et al.: "Fish gonadotropin and thyrotropin receptors : the evolution of glycoprotein hormone receptors in vertebrates."Comparative Biochemistry and Physiology B. 129. 441-448 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mita, M. et al.: "Glycoprotein hormone-receptor family in ovaries of starfish Asterina pectinifera"14th International Congress of Comparative Endocrinology. 1147-1152 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirai, T. et al.: "Fish gonadotropin receptors : molecular characterization and expression during gametogenesis."Fisheries Science. 68- Sup.1. 675-678 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mita, M. et al.: "cDNA cloning and functional analysis of a novel member of the glycoprotein hormone receptor family from a starfish Asterina pectinifera"Molecular Reproduction & Development. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirai, T. et al.: "Glycoprotein hormone receptor family in medaka (Oryzias latipes)"Fish Physiology and Biochemistry. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Rahman, K. et al.: "Gonadotropins, Gonadotropin receptors and their expressions during sexual maturation in yellowtail, a carangid fish"Fish Physiology and Biochemistry. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mita, M. et al.: "cDNA cloning and functional analysis of a novel member of the glycoprotein hormone receptor family from a starfish Asterina pectinifera"Mol.Rep.& Develop.. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oba, Y.et al.: "Fish gonadotropin and thyrotropin receptors : the evolution of glycoprotein hormone receptors in vertebrates"Comparative Biochemistry and Physiology B. 129. 441-448 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mita, M. et al.: "Glycoprotein hormone-receptor familly in ovaries of starfish Asterina pectinifera"14th International Congress of Comparative Endocrinology. 1147-1152 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirai, T. et al.: "Fish gonadotropin receptors : molecular characterization and expression during gametogenesis"Fisheries Science. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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