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The study on functional diversification of multiple family peptides in a mollusc, Aplysia

Research Project

Project/Area Number 13640682
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MATSUSHIMA Osamu  Hiroshima Univ. Graduate School of Science , Professor, 大学院・理学研究科, 教授 (80127711)

Co-Investigator(Kenkyū-buntansha) MORISHITA Fumihiro  Hiroshima University, Faculty of Science, Research Associate, 大学院・理学研究科, 助手 (20210164)
FUKUKAWA Yasuo  Hiroshima University, Faculty of Science, Associate Professor, 大学院・理学研究科, 助教授 (40209169)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsfamily peptides / neuropathies / inactivation / diversity / degrading enzyme / peptidase / deamidase / demidation / 多様性 / アメフラシ
Research Abstract

Three Aplysia peptide families were investigated for the functional diversity of their members. (1) Each antibody revealed the presence of immunoreactive cells and fibers in various tissues and organs of Aplysia, suggesting that these family peptides have versatile functions. (2) Effectiveness of the petides on some assay system was different between members of each family. This may indicate the occurrence of functional diversification among the family members. (3) Enzymatic degradation is one of the major inactivation mechanisms for peptides. Stability with the degrading enzymes and bioactivity of fragment peptides formed by the enzyme reactions may be the significant factors bearing functional diversification of the family members. One of the AMRP family members, GAPRFV amide, was degraded differentially by the hemolymph and the ganglionic membrane. A cardioactive D-amino acid-containing peptide, NdWFamide (Asn-D-Trp-Phe-NH_2), was inactivated by the deamidase reaction during incubation with the membrane preparation, but stable in the hemolymph. These results suggest that the mode of peptide degradation depends on their mode of action, neurotransmitters or humoral factors. It will be necessary to assess the possibility that the occurrence of multiple family peptides, their posttranslational modifications, and the mode of degradation may contribute to functional diversification of peptides.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Sasaki, K. et al.: "Structural and functional diversities of the Aplysia Mytilus inhibitory peptide-related peptides"Peptides. 23. 1959-1965 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanemaru, K. et al.: "Aplysia cardioactive peptide (NdWFamide) enhances the L-type Ca2+ current of Aplysia ventricular myocytes"Peptides. 23. 1991-1998 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsushima, O. et al.: "A novel GGNG-related neuropeptide from the polychaete Perinereis vancaurica"Peptides. 23. 1379-1390 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "Identification of a vasopressin-like immunoreactive substance in hydra"Peptides. 24. 17-26 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "Deamidase inactivates a D-amino acid-containing Aplysia neuropeptide."Peptides. 24. 45-51 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "Distribution of the Aplysia cardioexcitatory peptide, NdWFamide, the central and peripheral nervous systems of Aplysia"Cell & Tissue Res.. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "NdWFamide : a novel excitatory peptide for the cardiovascular regulation of Aplysia"Peptides. 22. 183-189 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harafuji, N. et al.: "Enhancement of foot formation in Hydra by a novel epitheliopeptide, Hym-323"Development. 128. 437-446 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nomura, T. et al.: "Purification and some properties of alanine racemase from a bivalve mollusk Corbicula japonica"J. Exp. Zool.. 289. 1-9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsushima, O. et al.: "The GGNG-peptide family : annelid myoactive peptides"Perspective in Comp. Endocrinol.. 1. 905-909 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagahama, T. et al.: "Leech excitatory peptide (LEP), a member of the GGNG-peptides : Localization and possible function in a leech, Whitmania pigra"Recent Adv. In Comp. Endocrinol.. 1. 147-152 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wang, X.H.et al.: "A candidate for fish prolactin-releasing peptide isolated from Japanese crucian carp"Recent Adv. In Comp. Endocrinol.. 1. 29-38 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Furukawa, Y. et al.: "The enterins : a novel family of neuropeptides isolated from the enteric nervous system and CNS of Aplysia"J. Neurosci.. 21. 8247-8261 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yakehiro, M. et al.: "Novel mechanism of blocking axonal Na channels by three macrocyclic polyamine analogues and two spider toxins"Br. J. Pharmacol.. 132. 63-72 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, K. et al.: "The enterins inhibit the contractile activity of the anterior aorta of Aplysia kurodai"J. Exp. Biol.. 205. 3525-3533 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimizu, Y. et al.: "Cumulative inactivation and the pore domain in the Kvl channels"Pflugers Arch-Eur. J. Physiol.. 443. 720-730 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawada, T. et al.: "A novel tachykini-related peptide receptor : Sequence, genomic organization, and functional analysis"Eur. J. Biochem.. 269. 4238-4246 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, K. et al.: "Structural and functional diversities of the Aplysia Mytilus inhibitory peptide-related peptides"Peptides. 23. 1959-1965 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanemaru, K. et al.: "Aplysia cardioactive peptide (NdWFamide) enhances the L-type Ca current of Aplysia ventricular myocytes"Peptides. 23. 1991-1998 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsushima, O. et al.: "A novel GGNG-related neuropeptide from the polychaete Perinereis vancaurica."Peptides. 23. 1379-1390 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "Identification of a vasopressin-like immunoreactive substance in hydra."Peptides. 24. 17-26 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "Deamidase inactivates a D-amino acid-containing Aplysia neuropeptide"Peptides. 24. 45-51 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "Distribution of the Aplysia cardioexcitatory peptide, NdFamide, in the central and peripheral nervous systems"Cell & Tissue Res.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F. et al.: "NdWFamide : a novel excitatory peptide for the cardiovascular regulation of Aplysia."Peptides. 22. 183-189 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harafuji, N. et al.: "Enhancement of foot formation in Hydra by a novel NdFamide, Hym-323."Development. 128. 437-446 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nomura, T. et al.: "Purification and some properties of alanine racemes from a bivalve mollusk Corbicula japonica."J. Exp. Zool.. 289. 1-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsushima, O. et al.: "The GGNG-peptide family : annelid myoactive peptides."Perspective in Comp. Endorinol.. 1. 905-909 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagahama, T. et al.: "Leech excitatory peptide (LEP), a member of the GGNG-peptides : Localization and possible function in a leech, Whitmania pigra."Recent Adv in Comp. Endocrinol.. 1. 147-152 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Wang, X. H. et al.: "A candidate for fish prolactin-releasing peptide isolated from Japanese crucian carp."Recent Adv in Comp. Endocrinol. 1. 29-38 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Furukawa, Y. et al.: "The enterins: a novel family of neuropeptides isolated from the enteric nervous system and CNS of Aplysia."J. Neurosci.. 21. 8247-8261 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yakehiro, M. et al: "Novel mechanism of blocking axonal Na channels by three macrocyclic polyamine analogues and two spider toxins."Br. J. Pharmacol. 132. 63-72 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, K. et al.: "The enterins inhibit the contractile activity of the anterior aorta of Aplysia kurodai"J. Exp. Biol.. 205. 3525-3533 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimizu, Y. et al.: "Cumulative inactivation and the pore domain in the Kvl channels"Pflugers Arch-Eur. J. Physiol.. 443. 720-730 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawada, T. et al.: "A novel tachykini-related peptide receptor : Sequence, genomic organization, and functional analysis."Eur. J. Biochem.. 269. 4238-4246 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sasaki, K. et al.: "Structural and functional diversities of the Aplysia Mytilus inhibitory peptide-related peptides"Peptides. 23. 1959-1965 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanemaru, K. et al.: "Aplysia cardioactive peptide(NdWFamide) enhances the L-type Ca2+ current of Aplysia ventricular myocytes"Peptides. 23. 1991-1998 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsushima, O. et al.: "A novel GGNG-related neuropeptide from the polychaete Perinereis vancaurica"Peptides. 23. 1379-1390 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morishita, F. et al.: "Identification of a vasopressin-like immunoreactive substance in hydra"Peptides. 24. 17-26 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morishita, F. et al.: "Deamidase inactivates a D-amino acid-containing Aplysia neuropeptide"Peptides. 24. 45-51 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morishita, F. et al.: "Distribution of the Aplysia cardioexcitatory peptide, NdWFamide, the central and peripheral nervous systems of Aplysia"Cell & Tissue Res.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morishita, F.et al.: "NdWFarnide : A novel excitatory peptide involved in cardiovascular regulation of Aplysia"Peptides. 22. 183-189 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Furukawa, Y.et al.: "The enterins : A novel family of neuropeptides isolated from the enteric and central nervous system of Aplysia"J. Neurosci.. 21. 8247-8261 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsushima, O.et al.: "A novel GGNG-related neuropeptide from the polychaete Perinereis vancaurica"Peptides. (In press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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