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Molecular mechanisms of the nerve net formation in hedra primitive nervous system

Research Project

Project/Area Number 13640687
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionFukuoka Women's University

Principal Investigator

KOIZUMI Osamu  Fukuoka Women's Univ., Environmental Science, Professor, 人間環境学部, 教授 (50094777)

Co-Investigator(Kenkyū-buntansha) MINOBE Sumiko  Fukuoka Women's Univ., Environmental Science, Assistant, 人間環境学部, 助手 (80190718)
Project Period (FY) 2001 – 2013
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsdiffuse nervous system / hydra / nerve net formation / developmental neurobiology / [peptide / immunohistochemistry / antibody production / nerve differentiation / 免疫組織化学 / 抗体作成
Research Abstract

(1)Large scale screening of peptide signal molecules : According to our peptide project, the following ideas were clarified.
(i)Peptide molecules have developmental functions such as morphogenesis and cell differentiation in addition to the well known function, the transmission of neural signals.
(ii)Peptide molecules are epithelial peptides localized in epithelial cells in addition to neuropeptides localized in nerve cells.
(2)Chemical anatomy of the hydra nervous system using neuropeptide antibodies : We produced antibodies to all peptides which structures-were identified. Using these antibodies, we did chemical anatomy of hydra, and the detailed anatomical chart covering the whole body was able to be made.
(3)Molecular mechanisms of the nerve net formation : We identified peptide molecules controlling the nerve differentiation. Hym355 is a neuropeptide activating the nerve differentiation. LPW peptides are epithelial peptides inhibiting the nerve differentiation.
(4)Nerve cell culture system as a bia-assay system. We tried to establish the cell culture system of nerve cells. If it will be established, it will be used as a bioassay system to screen neuroactive substances or active substances influences on the nervous system. The present situation is a success of short term culture of single neurons.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] S.Shimizu: "The diffuse nerve net in Hydra body column represents a primitive enteric nervous system."J.comp.physiol.. in press. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] O.Koizumi: "Chemical anatomy of hydra nervous system using antibodies against hydra neuropeptides : a review"Hydrobiologia. in press. (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] F.Mirishita: "Identification of a vasopressin-like immunoreactive substance in hydra."Peptides. 24. 17-26 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Takahashi: "Identification of a new member of the GLWamide peptide family : physiological activity and cellular localization in cnidarian polyps."Comp.Biochem.Physiol.. 135B. 309-324 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] O.Koizumi: "Developmental neurobiology of hydra, a model animal of cnidarians"Can.J.Zool.. 80. 1678-1689 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] D.Asai: "Preparation of the antibody which discriminates the conformations of ligand-bound and ligand-free estrogen receptors."Peptide Science. 2000. 411-412 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Shirnizu, O.Koizurni, T.Fujisawa: "The diffuse nerve net in Hydra body column represents a primitive enteric nervous system"J.comphysiol.. (in Press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] O.Koizumi, N.Sato, C.Goto: "Chemical anatomy of hydra nervous system using antibodies against hydra neuropeptides : a review"Hydrobiologia. (in Press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asai, D., Koizurni._O., Mohri.S., Nakai, M., Yakabe, Y., Tokunaga, T, Nose, T., Shimohigashi, Y.: "Biochemical evaluation of hormonal activity of endocrine disruptors by sensing the estrogen receptor conformation changes"Peptide Science 2002. 127-130 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Morishita, F., Nitagai, Y., Furukawa, Y., Matsushima, 0., Takahashi, T., Hatta, M., Fujisawa, T., Tunamoto, T., Koizumi, O.: "Identification of a vasopressin-like immunoreactive substance in hydra"Peptides. 24. 17-26 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, T., Kobayakawa, Y., Muneoka, Y., Fujisawa, Y., Mohri, S., Hatta, M., Shimizu, H., Fujisawa, T., Sugiyama, T., Takahara, M., Yanagi, K., Koizumi._0.: "Identification of a new member of the GLWamide peptide family : physiological activity and cellular localization in cnidarian polyps"ComBiochem.Physiol.(B). 135. 309-324 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] O.Koizumi: "Developmental neurobiology of Hydra, a model animal of cnidarians"Can.J.Zool.. 80. 1678-1689 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asai, D., Koizumi, O., Shirou Mohri, Shimohigashi, Y.: "Preparation of the antibody which discriminates the conformations of ligand-bound and ligand-free estrogen receptors : Applications to the quantitative analysis of ligand bindings"Peptide Science. 2000. 411-412 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, T, Koizumi, O., Ariura, Y., Romanovitch, A., Bosch, T.C.G., Kobayakawa, Y., Mohri, S., Bode, H., Yum, S., Hatta, M., Fujisawa, T.: "A novel neuropeptide, Hym355, positively regulates neuron differentiation in hydra"Development. 127. 997-1005 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Minobe, S., Fei.K., Yan L., Sagas, M., Werle, M.J.: "Identification and charaterization of the epithelial polarity receptor "Frizzled" in Hydra vulgaris"Dev.Genes Evol.. 210. 258-262 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Takahashi: "Identification of a new member of the LWamide peptide family : physiological activity and cellular localization in Cnidrian polyps"Comparative biochemistry and Physiology. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] F.Morishita: "Identification of a vasopressin-like immunoreactive substance in hydra"Peptides. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] D.Asai: "Biochemical evaluation of hormonal activity of endocrine disruptors by sensing the estrogen receptor conformation changes"Biol. Bull. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] O.Koizumi: "Developmental neurobiology of hydra, a model animal of cnidarians"Canadian Journal of Zoology. 80. 1678-1689 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Shimohigashi: "Nuclear receptor binding assay by sensing changes in receptor Conformation"Environ. Sci.. 8. 3-4 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] D.Asai: "Preparation of the antibody which discriminates the conformations of ligand-bound and ligand-free estrogen receptors"Peptide Science. 2000. 411-412 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] O.Koizumi: "Developmental neurobiology of cnidarians (specially Hydra)"Can. J. Zool.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] D.Asai: "Preparation of the antibody which discriminates the conformations of ligand-bound and ligand-free estrogen receptors"Peptide Science. 2000. 411-412 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Takahashi: "Identification of a new member of the LWamide peptide family : Physiological activity and cellular localization in Cnidarian polyps"Biol. Bull. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Takahashi: "A novel neuropeptide, Hym355, positively regulates neuron differentiation In hydra"Development. 127. 997-1005 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] s.Minobe: "Identification and characterizatiuon of the epithelial polarity receptor "Frizzled" in Hydra vulgaris"Development, Genes and Evolution. 210. 258-262 (2000)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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