Formulation of hypotheses and numerical simulations for cytoskeletal remodeling in vascular endothelial cells under physiological conditions from the viewpoint of mechanics
| Project/Area Number |
13650083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Materials/Mechanics of materials
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
YAMADA Hiroshi Kyushu Institute of Technology, Associate Professor, 大学院・生命体工学研究科, 助教授 (00220400)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Cell mechanics / Vascular endothelial cell / Stress fiber / Cyclic deformation / Orientation / Numerical simulation |
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| Research Abstract |
It is hypothesized that a stress fiber in an adherent vascular endothelial cell orients in the direction in which the strain in the stress fiber is smaller than a certain limit under cyclic deformation. It is revealed that the orientation of a stress fiber depends on its location in a cell, e.g., one between two ends on the bottom surface, one between the apical surface and the bottom surface, one between two ends on the apical surface of a cell. Preliminary numerical simulations for polymerization and depolymerization taking account of concentration of actin and accumulated strain of an actin filament show that this method is useful to describe the formation and dissociation of a stress fiber under cyclic deformation.
Finite element analyses are performed for a cultured endothelial cell on a substrate of a silicone membrane and an endothelial cell on the inside of the vascular wall. The former is modeled as an isolated cell, which consists of the cytoplasm and the nucleus, adhering to a substrate. The latter is modeled as a repeated structure of adherent cell model with the cytoplasm and the nucleus which adheres to the vascular wall. These models are assumed as hyperelastic materials. The analysis results showed that the strain in an upper region of a cell becomes smaller when the ratio of the cell height to the cell diameter on the substrate surface becomes larger. The deformation of the nucleus is reproduced by the proposed model to a certain degree. The strain distribution in a cell for the latter model is complicated due to an existence of the nucleus.
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Report
(3 results)
Research Products
(3 results)
