| Project/Area Number |
13650848
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Gunma University |
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Principal Investigator |
SHIRAGAMI Naohiro Gunma University, Faculty of Engineering, Professor, 工学部, 教授 (30162770)
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| Co-Investigator(Kenkyū-buntansha) |
HAKODA Masaru Gunma University, Faculty of Engineering, Research Associate, 工学部, 助手 (00302456)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | animal cell / shear stress / cell attachment |
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| Research Abstract |
Dynamic process encountered animal cell culture is considered to be cell attachment, cell growth and separation processes. The purpose of this project is to clarify the efficient cell attachment method, the shear stress value for keeping cell growth and the critical shear stress value for separating cells from substrate, respectively.
1. Culture device is remodelled from a cone-and-plate viscometer.
2. Cell attachment time under no shear stress was the order of ten minutes by measuring the microscope CCD camera system.
3. It is cleared that there is no required time for attaching cells to substrate.
4. The growth rate was depended on its cell density by measuring the microscope CCD camera system.
5. Cells were separated from a substrate by the shear stress value of several Pa.
6. The shear stress range is newly observed so as to keep a constant cell density.
7. An animal cell reactor for a long keeping high cell density is newly proposed.
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