• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Preparation of Antibacterial Epidermal Skin Graft

Research Project

Project/Area Number 13650855
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

SHINKAI Masashige  School of Eng., Dept. of Chem. & Biotech., Lecturer, 大学院・工学系研究科, 講師 (70262889)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Takeshi  Nagoya Univ., Grad. School of Eng., Professor, 大学院・工学研究科, 教授 (10043324)
NAGAMUNE Teruyuki  School of Eng., Dept. of Chem. & Biotech., Professor, 大学院・工学系研究科, 教授 (20124373)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsEpidermal Skin Graft / Tissue Transplantation / Cell Engineering / Tissue Engineering / Antibacterial peptide / Gene Therapy / Medical Materials / Cell Surface Engineering
Research Abstract

1. Application antibacterial peptide-presenting skin sheet
Antibacterial activity of epitherial cell which was genetically modified with sapecin gene was examined. After transplanting of eptherial cells into mouse, E. coli or S. aureus was infected. Moreover, we constructed new gene which had sapecin gene, linker containing a thrombin digesting motif and the PDGF (blood platelet growth factor) receptor. After transfection by lipofection method, the cells were dyed with the fluorescence antibody to the Myc tag which is a marker. However, the antibacterial activity was not observed, because peptide production was very low. Then, BAM-antibacterial peptide which was able to anchor on the cell surface was prepared, and its antibacterial activity was evaluated. When BAM-antibacterial peptide was added to the culture medium of mouse fibroblast cell, antibacterial peptide was able to be shown to the cell surface in several minutes. When E coli. culture medium was contacted into this cell, antibacterial peptide was released gradually and. On the other hand, BAM-antibacterial peptide was easily anchored on mouse outer skin and also antibacterial activity was observed against E. coli.
2. Establishment of the preparation method of cultured skin cell sheet
The biodegradable hydrogels were prepared though crosslinking of gelatin with transglutaminase (TGase). We found that the concentration of 5wt5 gelatin and 1 unit/ml TGase were optimum for the proliferation of NIH/3T3 fibroblast. The cell proliferation was enhanced by incorporation of cell adhesion factors in to gelatin hydorgels. Synthetic peptide, RGDLLQ, were added to the gelatin solution where LLQ motif is a substrate of TGase by virtue of a glutamine residue. These results suggest that TGase-mediated incorporation of cell adhesion factors into gelatin matrices enhanced cell proliferation and this novel biomaterial is a potent tool for wound dressing or tissue engineering.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] A.Ito, A.Mase, Y.Takizawa, M.Shinkai, H.Honda, K.Hata, M.Ueda, T.Kobayashi: "Transglutaminase-mediated gelatin matrices incorporating cell adhesion factors as a biomaterial for tissue engineering"Journal of Bioscience and Bioengineering. 95. 196-199 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Ito, A. Mase, Y. Takizawa, M. Shinkai, H. Honda, K. Hata, M. Ueda, T. Kobayashi: "Transglutaminase-mediated gelatin matrices incorporating cell adhesion factors as a biomaterial for tissue engineering"Journal of Bioscience and Bioengineering. 95. 196-199 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Ito, A.Mase, Y.Takizawa, M.Shinkai, H.Honda, K.Hata, M.Ueda, T.Kobayashi: "Transglutaminase-mediated gelatin matrices incorporating cell adhesion factors as a biomaterial for tissue engineering"Journal of Bioscience and Bioengineering. 95. 196-199 (2003)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi