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Biodesulfurization using thermophilic bacteria and identification of bds genes

Research Project

Project/Area Number 13650859
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionWaseda University

Principal Investigator

KIRIMURA Kohtaro  KIRIMURA,Kohtaro, 理工学部, 教授 (90195412)

Co-Investigator(Kenkyū-buntansha) USAMI Shoji  School of Science and Engineering, Professor, 理工学部, 教授 (50063508)
KINO Kuniki  School of Science and Engineering, Professor, 理工学部, 教授 (60318764)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBiotechnology / Bioengineering / Bioprocess / Microbial function / Biodesulfurization / Thermophilic bacteria / Dibenzothiophene / Flavin reductase / 環境微生物 / Mycobacterium / 有機硫黄化合物 / biodesulfurization / Bacillus subtilis / bioconversion / dibenzothiophene / diesel oil / Mycobacterium phlei / naphthothiophene / petroleum
Research Abstract

Recalcitrant organosulfur compounds such as dibenzothiophene (DBT) derivatives in light gas oil (LGO) cannot be removed by hydrodesulfurization using metallic catalysts. The thermophilic bacteria, Bacillus subtilis WU-S2B and Mycobacterium phlei WU-F1, were isolated for its ability to grow on DBT as the sole source of sulfur at 50℃. In addition to DBT, WU-S2B and WU-F1 also could desulfurize alkylated DBTs such as 4, 6-dimethyl DBT through a sulfur-specific degradation pathway with the selective cleavage of carbon-sulfur bonds. Moreover, when resting cells of WU-F1 were incubated at 45℃ with two types of hydrodesulfurized LGOs in the reaction mixtures containing 50%(v/v)oils, the biodesulfurization reduced the sulfur contents from 120 to 50 ppm S (F-LGO) and from 34 to 15 ppm S (X-LGO), respectively. Gas chromatography analysis with an atomic emission detector revealed that the peaks of alkylated DBTs including 4-methyl DBT, 4, 6-dimethyl DBT, and 3, 4, 6-trimethyl DBT significantly decreased after the biodesulfurization. The DBT-desulfurization genes, bdsABC, were cloned from these two bacteria, and the flavin reductase genes, frb and frm, were also cloned from WU-S2B and Wu-F1, respectively. The recombinant WU-F1 carrying one more set of bdsABC and frm was constructed, and the desulfurizing activity of the recombinant strain was 2. 2-fold higher than that of the wild type strain WU-F1.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Kohtaro Kirimura et al.: "Identification and functional analysis of the genes encoding dibenzothiophene-desulfurizing enzymes from thermophilic bacteria"Appl.Microbiol.Biotechnol.. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura et al.: "Cloning of a gene encoding flavin reductase coupling with dibenzothiophene monooxygenase through coexpression screening using indigo production as selective indication"Biochem. Biophys. Res. Commun.. 313. 570-575 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura et al.: "Thermophilic biodesulfurization of hydrodesulfurized light gas oils by Mycobacterium phlei WU-F1"FEMS Microbiol. Lett.. 221. 137-142 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura et al.: "Biodesulfurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by rhodococcus sp. strain WU-K2R"Appl. Environ. Microbiol.. 68. 3867-3872 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura et al.: "Thermophilic biodesulfurization of naphthothiophene and 2-ethylnaphthothiophene by a dibenzothiophene-desulfurizing bacterium, Mycobacterium phlei WU-F1"Appl.Microbiol.Biotechnol.. 58. 237-240 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura et al.: "Thermophilic biodesulfurization of dibenzothiophene and its derivatives by Mycobacterium phlei WU-F1"FEMS Microbiol. Lett.. 204. 129-133 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura, et al.: "Identification and functional analysis of the genes encoding dibenzothiophene-desulfurizing enzymes from thermophilic bacteria"Appl.Microbiol.Biotechnol.. (In Press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura, et al.: "Cloning of a encoding flavin reductase coupling with dibenzothiophene monooxygenase through coexpression screening indigo production selective indication"Biochem.Biophys.Res.Commun.. 313. 570-575 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura, et al.: "Thermophilic biodesulfurization of hydrodesulfurized light gas oils by Mycobacterium Phlei WU-F1"FEMS Microbiol.Lett.. 221. 137-142 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura, et al.: "Biodesulfurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by Rhodococcus s strain WU-K2R"Appl.Envron.Microbiol.. 68. 3867-3872 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura, et al.: "Thermophilic biosedulfurization of naphthothiophene and 2-ethylnaphthothiophene by a dibenzothiophene-desulfurizing bacterium, Mycobacterium phlei WU-F1"Appl.Microbiol.Biotechnol.. 58. 237-240 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kohtaro Kirimura, et al.: "Thermophilic biodesulfurization of dibenzothiophene and its derivatives by Mycobacterium phlei WU-F1"FEMS Microbiol.Lett.. 204. 129-133 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Furuya, S.Takahashi, Y.IShii, K.Kino, K.Kirimura: "Cloning of a Gene Encoding Flavin Reductase Coupling with Dibenzothiophene Monooxygenase through Coexpression Screening Using Indigo Production --"Biochem.Biophys.Res.Commun.. 313(2). 570-575 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Furuya, K.Kirimura, K.Kino, S.Usami: "Thermophilic Biodesulfurization of Naphthothiophene and 2-Ethylnaphthothiophene by -Mycobacterium phlei WU-F1"Appl. Microbiol. Biotechnol.. 58(2). 237-240 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Kirimura, T.Furuya, R.Sato, Y.Ishii, K.Kino, S.Usami: "Biodesulfurization of Naphthothiophene and Benzothiophene through Selective Cleavage -by Rhodococcus sp. Strain WU-K2R"Appl. Environ. Microbiol.. 68(6). 3867-3872 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Furuya, K.Kirimura, et al.: "Thermophilic biodesulfurization of dibenzothiophene and its derivatives by Mycobacterium phlei WU-Fl"FEMS Microbiol. Lett.. 204(1). 129-133 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Furuya, K.Kirimura, et al.: "Thermophilic biodesulfurization of naphthothiophene and 2-ethylnaphthothiophene by a dibenzothiophene-desulfurizing bacterium, Mycobacterium phlei WU-F1"Appi. Microbiol. Biotechnol. 58(2). 237-240 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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