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Development of Biosensing Method Using Electrochemical AC Impedance Measurement

Research Project

Project/Area Number 13650884
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 工業物理化学
Research InstitutionOsaka University

Principal Investigator

KUWABATA Susumu  Osaka University Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (40186565)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsbiosensor / AC impedance measurement / glucose oxidase / interference material / electron mediator / グルコースオキシターゼ / 複素インピーダンスプロット / 妨害物質
Research Abstract

Cyclic voltammetry and electrochemical AC impedance measurements were made by using a glassy carbon electrode in electrolyte solution (pH 7) containing electron mediator and glucose oxidase. The electron mediators used in this study were ferrocenecarboxylic acid and osmium bipyridine complex. In case of using the electrolyte solution containing the electron mediator alone, impedance plots gave linear relation with a slope angle of 45^○, implying that diffusion of the redox species controlled the reaction rate. When glucose oxidase and glucose were added to the solution, impedance plots with a semicircle shape were obtained and its diameter decreased with an increase in concentration of glucose. This behavior indicated that the electron transfer step determined the reaction rate. Since the decrease in diameter of the semicircle of plots means an increase in the reaction rate, then this principle allowed it to establish a method to determine glucose concentration from the impedance plots.
It was, furthermore, found that the AC impedance measurement was useful sensing method with avoiding infuence of interference material in the solution. The biosensor with use of ferrocenecarboxylic acid could avoid influence of ascorbic acid. If osmium bipyridine complex was used as an electron mediator, influence of uric acid as well as ascorbic acid was erased in the obtained data. Those results strongly suggested that new biosensing way would be developed with use of electrochemical AC impedance measurements.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Kuwabata, Susumu: "Novel Utilization of Impedance Measurement for Electrochemical Biosensing Aiming at Elimination of Influence by Interference Materials"Chemistry Letters. 32. 52-53 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kuwabata, Susumu: "Novel Utilization of Impedance Measurement for Electrochemical Biosensing Aiming at Elimination of Influence by Interference Materials"Chemistry Letter. 32(1). 52-53 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kuwabata, Susumu: "Novel Utilization of Impedance Measurement for Electrochemical Biosensing Aiming at Elimination of Influence by Interference Materials"Chemistry Letters. 32・1. 52-53 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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